| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 626,700 | A→G | 6.3% | T205A (ACA→GCA) | ycaQ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 626,700 | 0 | A | G | 6.3% | 392.0 / 13.2 | 143 | T205A (ACA→GCA) | ycaQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (62/72); new base G (3/6); total (65/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 157 total aligned reads displayed.
CCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGAACAGTGGCTGG > minE/626630‑626769 | ccAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGACGATGAGCGCGATCTCGTTTCGCaaa > 1:2732809/1‑71 (MQ=255) cAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa < 1:2431103/70‑1 (MQ=255) cAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa > 1:3051420/1‑70 (MQ=255) cAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa < 1:3449168/70‑1 (MQ=255) aGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa > 1:215403/1‑69 (MQ=255) aGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACa < 1:1973778/71‑1 (MQ=255) gcgcGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAg < 1:3484304/71‑1 (MQ=255) cgcgTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAg > 1:3616795/1‑70 (MQ=255) cgcgTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAg < 1:179120/70‑1 (MQ=255) gcgTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAg > 1:1905140/1‑69 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAg > 1:3886900/1‑70 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAg > 1:980906/1‑70 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAg > 1:1954360/1‑70 (MQ=255) tATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAg > 1:1847141/1‑70 (MQ=255) aTGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa > 1:2542816/1‑70 (MQ=255) aTGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa > 1:1135134/1‑70 (MQ=255) tttAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatca < 1:2810462/71‑1 (MQ=255) ttAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat > 1:4423463/1‑71 (MQ=255) ttAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat > 1:3163914/1‑71 (MQ=255) tAACTCACCGTGTCATGCCTGAGTGGAATGATGAGCGCGATCTCGTTTCGCAAGCAg < 1:1840279/57‑1 (MQ=255) tAACTCACCGTGTCATGCCTGAGTGGAATGATGAGCGCGATCTCGTTTCGCAAGCAGAAGCAGAAatcat < 1:2784506/70‑1 (MQ=255) tAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat > 1:2351352/1‑70 (MQ=255) aaCCCACCGTGTCATGCCTGATTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcatc < 1:4342943/70‑1 (MQ=255) aaCCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcatc < 1:3883832/70‑1 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCa > 1:3596656/1‑59 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa < 1:292902/61‑1 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa < 1:3312985/61‑1 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa < 1:1172046/61‑1 (MQ=255) cccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcat < 1:489116/67‑1 (MQ=255) ccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca > 1:612569/1‑68 (MQ=255) ccACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGc < 1:4080274/71‑1 (MQ=255) cGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTgg < 1:2159232/70‑1 (MQ=255) gtgtCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa < 1:2666423/55‑1 (MQ=255) gtCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGa < 1:1573366/53‑1 (MQ=255) gtCATGCCTGACTGGGATGATGAGAGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca < 1:565100/61‑1 (MQ=255) tCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa < 1:3147470/70‑1 (MQ=255) tCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATa < 1:1392419/69‑1 (MQ=255) tCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAAc < 1:4147106/71‑1 (MQ=255) cATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa > 1:1932846/1‑69 (MQ=255) cATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa > 1:2252527/1‑69 (MQ=255) cATGCCTGACTGCGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa < 1:3913948/69‑1 (MQ=255) aTGCCTGAGTGGAATGATGAGCGCGATCTCGTTTCGCAAGCAg < 1:845258/43‑1 (MQ=255) aTGCCTGAGTGGAATGATGAGCGCGATCTCGTTTCGCAAGCAGAAGCAGAAATCATCATGCTGGATAACAg > 1:3562418/1‑71 (MQ=38) aTGCCTGAGTGGAATGATGAGCGCGATCTCGTTTCGCAAGCAGAAGCAGAAATCATCATGCTGGATAACAg > 1:1512972/1‑71 (MQ=38) tGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca > 1:2118663/1‑57 (MQ=255) tGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa < 1:2296613/67‑1 (MQ=255) gCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa > 1:334367/1‑38 (MQ=255) gCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCaaa > 1:2730561/1‑38 (MQ=255) gACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGaaa > 1:1266206/1‑46 (MQ=255) gACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGaaa > 1:623408/1‑46 (MQ=255) gACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGaaa > 1:2841969/1‑46 (MQ=255) cTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGa < 1:4301114/36‑1 (MQ=255) cTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca > 1:4581606/1‑50 (MQ=255) cTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAAtcatca > 1:546094/1‑50 (MQ=255) cTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGt < 1:2044423/70‑1 (MQ=255) ggATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGcc < 1:3300234/71‑1 (MQ=255) ggATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGc < 1:230713/70‑1 (MQ=255) tgatgaGCGCGATCTCGTTTCGCAAGCAGAAGCAGAAATcatcat < 1:3928808/45‑1 (MQ=255) tgatgaGCGCGATCTCGTTTCGCAAACAGAAGCAGaa < 1:1808275/37‑1 (MQ=255) tgatgaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAatcatc < 1:2616816/43‑1 (MQ=255) gatgaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCtcg > 1:1230292/1‑68 (MQ=255) gatgaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTgg > 1:2055527/1‑70 (MQ=255) gatgaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTgg > 1:3707288/1‑70 (MQ=255) atgaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAat > 1:1736457/1‑37 (MQ=255) atgaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAat > 1:719798/1‑37 (MQ=255) atgaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATcatcat < 1:750185/43‑1 (MQ=255) atgaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATcatcat < 1:219156/43‑1 (MQ=255) atgaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa < 1:689913/52‑1 (MQ=255) atgaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTggg < 1:44254/70‑1 (MQ=255) tgaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGaa > 1:3508993/1‑71 (MQ=255) gaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAAc > 1:945866/1‑51 (MQ=255) gaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACa > 1:1353276/1‑52 (MQ=255) gaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACa > 1:2904671/1‑52 (MQ=255) gaGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACa > 1:3106391/1‑52 (MQ=255) aGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTg > 1:3735133/1‑44 (MQ=255) aGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAata < 1:122413/71‑1 (MQ=255) gcgcGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATa > 1:1591787/1‑47 (MQ=255) aTCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAata < 1:3826811/65‑1 (MQ=255) aTCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATAtt < 1:4654179/67‑1 (MQ=255) ctcGTTTCGCAAACAGAAGCAGAAATCATCATGCTgg < 1:4008794/37‑1 (MQ=255) ctcGTTTCGCAAACAGAAGCAGAAATCATCATGCTg > 1:912539/1‑36 (MQ=255) ctcGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa > 1:2400173/1‑41 (MQ=255) cGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATaa < 1:4526135/39‑1 (MQ=255) gTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGt > 1:2710288/1‑48 (MQ=255) gTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAata > 1:2969140/1‑60 (MQ=255) gTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCcgc > 1:412266/1‑66 (MQ=255) gTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGaa > 1:2264450/1‑69 (MQ=255) gTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGaa > 1:4256186/1‑69 (MQ=255) gTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGaa < 1:1875589/69‑1 (MQ=255) gTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGaa < 1:95280/69‑1 (MQ=255) gTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGaa > 1:1257196/1‑69 (MQ=255) tttCGCAAACAGAAGCAGAAATCATCATGCTGGATAAc < 1:1680711/38‑1 (MQ=255) tttCGCAAACAGAAGCAGAAATCATCATGCTGGATAACa > 1:3786164/1‑39 (MQ=255) tCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGAACAGtg > 1:4067363/1‑71 (MQ=255) cGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGAACAg > 1:4548433/1‑68 (MQ=255) gCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGAACAGtg < 1:3420503/69‑1 (MQ=255) gCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGAACAGtg < 1:3244441/69‑1 (MQ=255) aaCAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGAACAGt > 1:3260793/1‑65 (MQ=255) aCAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGAACAGtggctgg > 1:2096619/1‑70 (MQ=255) aCAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGAACAGtggctgg > 1:2002684/1‑70 (MQ=255) | CCAGCGCGTTTATGATTTAACCCACCGTGTCATGCCTGACTGGGATGATGAGCGCGATCTCGTTTCGCAAACAGAAGCAGAAATCATCATGCTGGATAACAGTGCGCGTAGCCTGGGAATATTCCGCGAACAGTGGCTGG > minE/626630‑626769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |