| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 130,099 | G→A | 6.9% | L93L (TTG→TTA) | yadE → | predicted polysaccharide deacetylase lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 130,099 | 0 | G | A | 6.9% | 176.2 / 2.7 | 58 | L93L (TTG→TTA) | yadE | predicted polysaccharide deacetylase lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (30/24); new base A (2/2); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
TTGGCAAAGGTTTTATCGATAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATACGCCGGTCTATAACGC > minE/130029‑130167 | ttggcAAAGGTTTTATCGATAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTg > 1:9277/1‑71 (MQ=255) tggcAAAGGTTTTATCGATAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTgg > 1:3061023/1‑71 (MQ=255) gtaaaGGGTTTATCGATAAAGGCCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGATGGTTTaggt < 1:52198/69‑2 (MQ=255) gcAAAGGTTTTATCTATAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTg < 1:4323570/68‑1 (MQ=255) aaGGTTTTATCGATAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGAc > 1:1589090/1‑71 (MQ=255) aGGTTTTATCGATAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGAcc > 1:82188/1‑71 (MQ=255) gTTTTATCGATAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTc < 1:1014767/71‑1 (MQ=255) ttATCGATAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTcaa > 1:2349897/1‑70 (MQ=255) gATAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGcc < 1:4513521/71‑1 (MQ=255) gATAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGc > 1:3747990/1‑70 (MQ=255) tAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGc > 1:3421374/1‑71 (MQ=255) gTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCt < 1:2409300/67‑1 (MQ=255) gTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAg > 1:1386807/1‑70 (MQ=255) aTCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGATGGTTTaggt < 1:1952741/47‑2 (MQ=255) aTCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTaa < 1:1423164/70‑1 (MQ=255) aTCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTaa < 1:981645/70‑1 (MQ=255) aTCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAAt < 1:4014585/71‑1 (MQ=255) aTCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAAt < 1:4447047/71‑1 (MQ=255) aTCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAAt < 1:557386/71‑1 (MQ=255) tctcGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTcaac < 1:3828747/55‑1 (MQ=255) ctcGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGATTGTTTAGGTGACCTca < 1:2398037/52‑1 (MQ=255) ctcGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGATGGTTTAGGTGACCTca < 1:1269985/52‑1 (MQ=255) ctcGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAAt > 1:4361102/1‑69 (MQ=255) ctcGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAAt < 1:4348823/69‑1 (MQ=255) tcGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGATGGTTTAGGTGACCTCAACAAGCCGCTGAGt > 1:889589/1‑65 (MQ=255) tcGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAAt > 1:3317072/1‑68 (MQ=255) tcGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAAt > 1:582571/1‑68 (MQ=255) gAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTcaaca > 1:2644679/1‑52 (MQ=255) gAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGaa > 1:1489154/1‑71 (MQ=255) ccGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACtt < 1:2953094/71‑1 (MQ=255) ttCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTa < 1:3000970/57‑1 (MQ=255) ttCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGtt < 1:4281021/71‑1 (MQ=255) ttCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGtt < 1:4112166/71‑1 (MQ=255) tCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTa > 1:3364461/1‑71 (MQ=255) aGGGGCGACATAAAGTTGAAGACGGTTTGGGCGACCTcaa < 1:3111856/40‑1 (MQ=255) ggggCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCt < 1:4101462/71‑1 (MQ=255) gggCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTg < 1:113533/71‑1 (MQ=255) gACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGaaa < 1:1201703/71‑1 (MQ=255) gACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGaaa < 1:3496690/71‑1 (MQ=255) aCAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTgg > 1:3924519/1‑67 (MQ=255) cAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGa < 1:3979571/71‑1 (MQ=255) aaaaaGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGa > 1:1792451/1‑42 (MQ=255) aaaaaGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGa > 1:4635588/1‑70 (MQ=255) aaaaaGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGa > 1:1146204/1‑70 (MQ=255) aaaaaGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGa > 1:3714219/1‑70 (MQ=255) aaaaGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATa > 1:1850052/1‑71 (MQ=255) aaGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTgg > 1:2663256/1‑62 (MQ=255) tGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATACGCCgg > 1:4307321/1‑71 (MQ=255) gAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATACGCCGGt > 1:3010345/1‑71 (MQ=255) gAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATACGCCGGt > 1:742447/1‑71 (MQ=255) aGATGGTTTAGGTGACCTCAACAAGCCGCTGAGTAATCAGAACTTAATAAcc > 1:1387247/1‑52 (MQ=255) aGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGa > 1:614320/1‑41 (MQ=255) aCGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTa < 1:3941622/44‑1 (MQ=255) aCGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATACGCCGGTCta > 1:1863368/1‑70 (MQ=255) aCGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATACGCCGGTCta > 1:2493107/1‑70 (MQ=255) ggTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATACGCCGGTCtata < 1:889037/70‑1 (MQ=255) ggTTTGCGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATACGCCGGTCTATaa > 1:4388/1‑71 (MQ=255) gTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAg < 1:433865/54‑1 (MQ=255) gTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATACGCCGGTCTATaa > 1:950932/1‑70 (MQ=255) tttGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTa < 1:4230709/40‑1 (MQ=255) ttGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCt > 1:1156639/1‑46 (MQ=255) ttGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATa > 1:895202/1‑55 (MQ=255) ttGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATACGCCGGTCTATAAcgc > 1:2473090/1‑71 (MQ=255) | TTGGCAAAGGTTTTATCGATAAAGGTCATCTCGAGCCGGTTCAGGGGCGACAAAAAGTTGAAGACGGTTTGGGCGACCTCAACAAGCCGCTGAGTAATCAGAACTTAGTTACCTGGAAAGATACGCCGGTCTATAACGC > minE/130029‑130167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |