| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 667,493 | C→T | 6.3% | R277R (CGC→CGT) | uup → | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 667,493 | 0 | C | T | 6.3% | 191.5 / 2.1 | 63 | R277R (CGC→CGT) | uup | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (31/28); new base T (2/2); total (33/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGC > minE/667424‑667559 | aCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGcc > 1:3303629/1‑71 (MQ=255)aCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGc < 1:3271787/70‑1 (MQ=255)aCAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGc < 1:2480309/70‑1 (MQ=255) aaaaTGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGt < 1:1431948/71‑1 (MQ=255) aaaTGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGt < 1:670062/70‑1 (MQ=255) aaaTGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGt < 1:148845/70‑1 (MQ=255) aaaTGCCCAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTa < 1:4261069/71‑1 (MQ=255) aTGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTAcc > 1:1635070/1‑71 (MQ=255) tGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTAccc > 1:804629/1‑71 (MQ=255) ccGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGcc < 1:2123047/63‑1 (MQ=255) aGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGt < 1:4048442/62‑1 (MQ=255) ttCTATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGa < 1:4415173/71‑1 (MQ=255) cGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAAt > 1:727020/1‑67 (MQ=255) cGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATg > 1:3374667/1‑68 (MQ=255) cGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGaa > 1:4445636/1‑66 (MQ=255) cGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcg > 1:3666757/1‑71 (MQ=255) gCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGTGGATCAAAGCACGCCGTACCCGTAATg < 1:1161460/63‑1 (MQ=255) cAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcgcg < 1:571235/71‑1 (MQ=255) aaCTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGTCGTACCCGTAATGAAGGCCGCGTa > 1:4101046/1‑71 (MQ=255) aCTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTAc < 1:2995456/71‑1 (MQ=255) tGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTAcg > 1:73043/1‑70 (MQ=255) ggCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTAcgcg > 1:790856/1‑71 (MQ=255) gcgcAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTAcgcg < 1:1236595/70‑1 (MQ=255) gAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGaa < 1:2763790/71‑1 (MQ=255) gAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGa > 1:4512229/1‑70 (MQ=255) gAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGa > 1:4419320/1‑70 (MQ=255) aaGAGGTGTGGATCCGCCAGGGTATCAAAGCACGCCTTACCCGTAATGAAGGCCGCGTACGCGCCCTg < 1:1572550/68‑1 (MQ=255) agagGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGaaa > 1:2482821/1‑70 (MQ=255) gagGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGc < 1:4429760/71‑1 (MQ=255) gagGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGc < 1:2233722/71‑1 (MQ=255) agGTGTGGATCCGCCAGGGTATCAAAGCACGCCGTACCCGTAATGAAGGcc < 1:1556726/51‑1 (MQ=255) gtgGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGaa > 1:1938652/1‑43 (MQ=255) gtgGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGc > 1:4687397/1‑71 (MQ=255) tgGATCCGCCAGGGTATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCg > 1:612012/1‑71 (MQ=255) tgGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATg < 1:4597708/40‑1 (MQ=255) tgGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCg > 1:4537728/1‑71 (MQ=255) tgGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCg < 1:1363592/71‑1 (MQ=255) ggATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGc < 1:1018047/64‑1 (MQ=255) gATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGt > 1:4432097/1‑70 (MQ=255) gATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTc > 1:2961117/1‑71 (MQ=255) gATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTc > 1:235584/1‑71 (MQ=255) ccGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTcgc > 1:1219184/1‑70 (MQ=255) ccGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTcgc > 1:422231/1‑70 (MQ=255) cGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTcgcg > 1:3524091/1‑70 (MQ=255) cGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTcgcg < 1:1827186/70‑1 (MQ=255) cAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACg < 1:4220268/71‑1 (MQ=255) cAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACg < 1:620776/71‑1 (MQ=255) aGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACgt > 1:670460/1‑71 (MQ=255) aGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACgt > 1:285942/1‑71 (MQ=255) ggggATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACgtg > 1:3237399/1‑71 (MQ=255) ggggATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACgtg > 1:3067255/1‑71 (MQ=255) gggATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGc < 1:420531/53‑1 (MQ=255) gggATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGc < 1:1241219/53‑1 (MQ=255) gggATCAAAGCACGCCGTACACGTAATGAAGGCCGCGTACGCGCCCTGAAAGc < 1:1708075/53‑1 (MQ=255) ggATCAAAGCACGTCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCAATg > 1:3508352/1‑56 (MQ=255) aTCAAAGCACGTCGTACCCGTAATGAAGGCCGCGTACGCTCCCTg < 1:2787302/45‑1 (MQ=255) aTCAAAGCACGTCGTACCCGTAATGAAGGCCGCGTACGCGCCCTg < 1:3073796/45‑1 (MQ=255) aTCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTg < 1:3393454/45‑1 (MQ=255) aTCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa < 1:1285663/71‑1 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGaaa > 1:4069045/1‑46 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAAc > 1:540355/1‑71 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAAc > 1:4055534/1‑71 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAAc > 1:3731924/1‑71 (MQ=255) aaaGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGAAGCGTCGCGAACGTGGTGAAc > 1:991389/1‑70 (MQ=255) aaGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTTGTGAACGt < 1:2128385/71‑1 (MQ=255) aaGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGt < 1:3826024/71‑1 (MQ=255) cACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTcgc > 1:3470851/1‑71 (MQ=255) | ACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGC > minE/667424‑667559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |