| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 718,738 | A→T | 10.9% | R24R (CGA→CGT) | mviN → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 718,738 | 0 | A | T | 10.9% | 136.4 / 10.3 | 55 | R24R (CGA→CGT) | mviN | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (30/19); new base T (3/3); total (33/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
AATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCTTTTTCGTCGCTTTTAAACTTC > minE/718670‑718808 | aaTTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACgagc > 1:1203267/1‑70 (MQ=255) tttATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACga < 1:2954195/67‑1 (MQ=255) tattaAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGc < 1:4233043/70‑1 (MQ=255) aaaTCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACgag < 1:1051987/61‑1 (MQ=255) aaaTCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACgag < 1:645559/61‑1 (MQ=255) aaaTCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGt > 1:459139/1‑71 (MQ=255) aaaTCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGt > 1:236087/1‑71 (MQ=255) tCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGc > 1:997994/1‑71 (MQ=255) tCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGc > 1:2733440/1‑71 (MQ=255) cgccgTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCt < 1:1200496/71‑1 (MQ=255) ccgTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCttt < 1:353019/71‑1 (MQ=255) gTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTc > 1:4139826/1‑57 (MQ=255) gTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTc > 1:1870173/1‑57 (MQ=255) tCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCt < 1:2162698/66‑1 (MQ=255) tCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTgg > 1:3766036/1‑70 (MQ=255) aGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCaa > 1:1226013/1‑49 (MQ=255) aGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCaa > 1:3717756/1‑49 (MQ=255) aGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTg > 1:3427953/1‑67 (MQ=255) aGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGgcg > 1:1313037/1‑70 (MQ=255) aGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGgcg > 1:148424/1‑70 (MQ=255) aGCTCGATGACCATGTTTTAGCGTGTGCTTGTCTTCGCACGAGAc > 1:3841133/1‑45 (MQ=38) gCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGgcg > 1:1862426/1‑69 (MQ=255) cTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCa < 1:866652/70‑1 (MQ=255) cTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAg > 1:120332/1‑71 (MQ=255) cGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAgg > 1:3483235/1‑70 (MQ=255) gATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCg < 1:1704225/50‑1 (MQ=255) gATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAgg < 1:1633076/69‑1 (MQ=255) aCCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGc < 1:895433/71‑1 (MQ=255) aTGTTTTCGCGTGTGCTTGGCTTCGCACGTGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCaa > 1:4568749/1‑70 (MQ=255) aTGTTTTCGCGTGTGCTTGGCTTCGCACGTGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAAc > 1:3461244/1‑71 (MQ=255) aTGTTTTCGCGTGTGCTTGGCTTCGCACGTGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAAc > 1:2137433/1‑71 (MQ=255) tGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATg < 1:2915531/65‑1 (MQ=255) tGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAAcc > 1:3625251/1‑71 (MQ=255) gTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGc < 1:3777060/66‑1 (MQ=255) gTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGc < 1:2142591/66‑1 (MQ=255) ttttCGCGTGTGCTTGGCTTCGCACGTGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCg < 1:3521133/70‑1 (MQ=255) ttttCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGa > 1:3466931/1‑71 (MQ=255) ttCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGa > 1:1527913/1‑59 (MQ=255) ttCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACg > 1:4002783/1‑71 (MQ=255) gtgtGCTTGGCTTCGCACGAGACGCAATTGTCGCCATAATCTTTGGCGCAGGGATgg > 1:3267241/1‑57 (MQ=255) gtgtGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCttt < 1:1756945/71‑1 (MQ=255) gtgtGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCttt < 1:627943/71‑1 (MQ=255) tgtgCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCtttt < 1:124691/71‑1 (MQ=255) tgtgCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCtttt > 1:267846/1‑71 (MQ=255) tgCTTGGCTTCGCACGTGACGCAATTGTCGCCAGAATCTTTg < 1:1816262/42‑1 (MQ=255) tgCTTGGCTTCGCACGTGACGCAATTGTCGCCAGAATCTTTg < 1:4322394/42‑1 (MQ=255) tgCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGAc > 1:3775546/1‑62 (MQ=255) tgCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCtttt < 1:3790095/69‑1 (MQ=255) tgCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCTTTTtc > 1:1759186/1‑71 (MQ=255) cTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAAcc > 1:2761402/1‑57 (MQ=255) ttGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCTTTTtcgtc > 1:3309052/1‑71 (MQ=255) ctgcGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCTTTTtcgtc < 1:2085510/64‑1 (MQ=255) tCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCTTTTTCGTCGCttt > 1:1253651/1‑70 (MQ=255) cGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGc > 1:916466/1‑45 (MQ=255) cGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCttt > 1:2862445/1‑58 (MQ=255) cGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCTTTTTCGTCGCttt < 1:2394096/69‑1 (MQ=255) gCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCTTTTTCGTCGCTTTTa > 1:1555948/1‑70 (MQ=255) gCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCTTTTTCGTCGCTTTTa < 1:3008751/70‑1 (MQ=255) gCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCTTTTTCGTCGCTTTTa < 1:2855956/70‑1 (MQ=255) gagaCGCAATTGTCGCCAGAATCTTTGGCTCAGGGATGGCAACCGACGCCTTTTTCGTCGCTTTTAAACtt < 1:2008971/71‑1 (MQ=255) agaCGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCTTTTTCGTCGCTTTTAAACTTc > 1:4156299/1‑71 (MQ=255) | AATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGATGGCAACCGACGCCTTTTTCGTCGCTTTTAAACTTC > minE/718670‑718808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |