| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 732,870 | G→A | 6.0% | V106I (GTT→ATT) | pabC → | 4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 732,870 | 0 | G | A | 6.0% | 205.6 / 2.5 | 66 | V106I (GTT→ATT) | pabC | 4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex |
| Reads supporting (aligned to +/- strand): ref base G (32/30); new base A (2/2); total (34/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
CAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCG > minE/732803‑732940 | cAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTa > 1:3983927/1‑71 (MQ=255)cAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTa > 1:1564840/1‑71 (MQ=255) ggTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGt < 1:4532924/62‑1 (MQ=255) tAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATc < 1:3408912/71‑1 (MQ=255) aGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATcc > 1:943274/1‑71 (MQ=255) aGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATcc > 1:937910/1‑71 (MQ=255) gTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCt > 1:822379/1‑71 (MQ=255) tgtcgggcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCt < 1:1478663/61‑1 (MQ=255) gcgggcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGca > 1:2440565/1‑71 (MQ=255) gcgggcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGca > 1:4479960/1‑71 (MQ=255) gcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACAt > 1:718915/1‑70 (MQ=255) gggTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATc > 1:1707451/1‑58 (MQ=255) ggTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGcaca < 1:351581/64‑1 (MQ=255) gTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGt < 1:1485609/45‑1 (MQ=255) aCAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGca < 1:4186542/59‑1 (MQ=255) cAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGtt > 1:987324/1‑71 (MQ=255) cAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGt < 1:2625868/70‑1 (MQ=255) aGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACAt < 1:2271220/60‑1 (MQ=255) gCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTg > 1:4397173/1‑71 (MQ=255) gCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTg > 1:61513/1‑71 (MQ=255) cacaTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGc > 1:3849099/1‑71 (MQ=255) acaTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGt < 1:1638911/38‑1 (MQ=255) caTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCg > 1:2701056/1‑70 (MQ=255) caTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGt < 1:1197894/71‑1 (MQ=255) caTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGt < 1:1206281/71‑1 (MQ=255) caTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGt < 1:2009230/71‑1 (MQ=255) ttGAACAGCGGACCGGAAACGCGGATTCTCTCCGTTACgg < 1:2138506/40‑1 (MQ=255) tGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTa > 1:3447369/1‑36 (MQ=255) gAACAGCGGACCGGCAACGCGGATTCTCTCCGTTa > 1:4111270/1‑35 (MQ=255) gAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAAc < 1:2229673/70‑1 (MQ=255) gAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAAc < 1:4193023/70‑1 (MQ=255) gAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAAc < 1:3954714/70‑1 (MQ=255) gAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAAc > 1:2515187/1‑70 (MQ=255) aaCAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACg < 1:3250187/70‑1 (MQ=255) gCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAggg > 1:3972908/1‑70 (MQ=255) cGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGa > 1:3546997/1‑51 (MQ=255) cGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGa > 1:77027/1‑71 (MQ=255) ggACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGAt < 1:2929509/71‑1 (MQ=255) ggACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGAt < 1:248580/71‑1 (MQ=255) gACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAAc < 1:2493041/62‑1 (MQ=255) ccGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGa > 1:1076154/1‑62 (MQ=255) ccGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGa > 1:440661/1‑62 (MQ=255) ccGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTAc > 1:820459/1‑71 (MQ=255) gCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTaa > 1:566333/1‑56 (MQ=255) gCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGtt < 1:2031406/71‑1 (MQ=255) gCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGtt > 1:1842396/1‑71 (MQ=255) gCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGtt < 1:2545117/71‑1 (MQ=255) gCAACGCGGATTCTCTCCATTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGa < 1:4429095/59‑1 (MQ=255) gCAACGCGGATTCTCTCCATTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGa < 1:671115/59‑1 (MQ=255) cAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGt > 1:2330597/1‑69 (MQ=255) aCGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTaa > 1:436420/1‑53 (MQ=255) gATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCt > 1:3286761/1‑69 (MQ=255) gATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTaa < 1:439920/71‑1 (MQ=255) aTTCTCTCCGTTACGGCTTATCCTGCACATTACGAcc < 1:1860275/37‑1 (MQ=255) aTTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAg < 1:3622666/71‑1 (MQ=255) tctctcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGATGGGATTACGTTGGCGCTAAGcc > 1:325106/1‑71 (MQ=255) tctctcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGcc > 1:3607785/1‑71 (MQ=255) tctctcCATTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAAGGGATGATGTTGGCGCTAAGcc > 1:2787581/1‑71 (MQ=255) tctctcCATTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAAGGGATGATGTTGGCGCTAAGcc > 1:1421941/1‑71 (MQ=255) tcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGt < 1:1315391/39‑1 (MQ=255) tcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAg > 1:1611487/1‑45 (MQ=255) tcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGccc < 1:3926819/68‑1 (MQ=255) tcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCgg > 1:3075498/1‑70 (MQ=255) cGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTg < 1:3184306/70‑1 (MQ=255) cGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGc < 1:4496159/71‑1 (MQ=255) cGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGc < 1:1152506/71‑1 (MQ=255) gTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCg < 1:3857659/71‑1 (MQ=255) gTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCg < 1:1861470/71‑1 (MQ=255) | CAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCG > minE/732803‑732940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |