Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 732,870 G→A 6.0% V106I (GTT→ATT)  pabC → 4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE732,8700GA 6.0% 205.6 / 2.5 66V106I (GTT→ATT) pabC4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base G (32/30);  new base A (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

CAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCG  >  minE/732803‑732940
                                                                   |                                                                      
cAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTa                                                                     >  1:3983927/1‑71 (MQ=255)
cAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTa                                                                     >  1:1564840/1‑71 (MQ=255)
       ggTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGt                                                                       <  1:4532924/62‑1 (MQ=255)
         tAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATc                                                            <  1:3408912/71‑1 (MQ=255)
          aGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATcc                                                           >  1:943274/1‑71 (MQ=255)
          aGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATcc                                                           >  1:937910/1‑71 (MQ=255)
           gTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCt                                                          >  1:822379/1‑71 (MQ=255)
            tgtcgggcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCt                                                                <  1:1478663/61‑1 (MQ=255)
              gcgggcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGca                                                       >  1:2440565/1‑71 (MQ=255)
              gcgggcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGca                                                       >  1:4479960/1‑71 (MQ=255)
                  gcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACAt                                                    >  1:718915/1‑70 (MQ=255)
                      gggTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATc                                                            >  1:1707451/1‑58 (MQ=255)
                       ggTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGcaca                                                     <  1:351581/64‑1 (MQ=255)
                        gTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGt                                                                       <  1:1485609/45‑1 (MQ=255)
                          aCAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGca                                                       <  1:4186542/59‑1 (MQ=255)
                           cAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGtt                                          >  1:987324/1‑71 (MQ=255)
                           cAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGt                                           <  1:2625868/70‑1 (MQ=255)
                            aGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACAt                                                    <  1:2271220/60‑1 (MQ=255)
                             gCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTg                                        >  1:4397173/1‑71 (MQ=255)
                             gCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTg                                        >  1:61513/1‑71 (MQ=255)
                              cacaTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGc                                       >  1:3849099/1‑71 (MQ=255)
                               acaTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGt                                                                       <  1:1638911/38‑1 (MQ=255)
                                caTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCg                                      >  1:2701056/1‑70 (MQ=255)
                                caTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGt                                     <  1:1197894/71‑1 (MQ=255)
                                caTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGt                                     <  1:1206281/71‑1 (MQ=255)
                                caTTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGt                                     <  1:2009230/71‑1 (MQ=255)
                                  ttGAACAGCGGACCGGAAACGCGGATTCTCTCCGTTACgg                                                                  <  1:2138506/40‑1 (MQ=255)
                                   tGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTa                                                                     >  1:3447369/1‑36 (MQ=255)
                                    gAACAGCGGACCGGCAACGCGGATTCTCTCCGTTa                                                                     >  1:4111270/1‑35 (MQ=255)
                                    gAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAAc                                  <  1:2229673/70‑1 (MQ=255)
                                    gAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAAc                                  <  1:4193023/70‑1 (MQ=255)
                                    gAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAAc                                  <  1:3954714/70‑1 (MQ=255)
                                    gAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAAc                                  >  1:2515187/1‑70 (MQ=255)
                                     aaCAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACg                                 <  1:3250187/70‑1 (MQ=255)
                                         gCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAggg                             >  1:3972908/1‑70 (MQ=255)
                                          cGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGa                                               >  1:3546997/1‑51 (MQ=255)
                                          cGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGa                           >  1:77027/1‑71 (MQ=255)
                                           ggACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGAt                          <  1:2929509/71‑1 (MQ=255)
                                           ggACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGAt                          <  1:248580/71‑1 (MQ=255)
                                            gACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAAc                                  <  1:2493041/62‑1 (MQ=255)
                                              ccGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGa                                >  1:1076154/1‑62 (MQ=255)
                                              ccGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGa                                >  1:440661/1‑62 (MQ=255)
                                              ccGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTAc                       >  1:820459/1‑71 (MQ=255)
                                                 gCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTaa                                   >  1:566333/1‑56 (MQ=255)
                                                 gCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGtt                    <  1:2031406/71‑1 (MQ=255)
                                                 gCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGtt                    >  1:1842396/1‑71 (MQ=255)
                                                 gCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGtt                    <  1:2545117/71‑1 (MQ=255)
                                                 gCAACGCGGATTCTCTCCATTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGa                                <  1:4429095/59‑1 (MQ=255)
                                                 gCAACGCGGATTCTCTCCATTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGa                                <  1:671115/59‑1 (MQ=255)
                                                  cAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGt                     >  1:2330597/1‑69 (MQ=255)
                                                    aCGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTaa                                   >  1:436420/1‑53 (MQ=255)
                                                         gATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCt              >  1:3286761/1‑69 (MQ=255)
                                                         gATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTaa            <  1:439920/71‑1 (MQ=255)
                                                          aTTCTCTCCGTTACGGCTTATCCTGCACATTACGAcc                                             <  1:1860275/37‑1 (MQ=255)
                                                          aTTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAg           <  1:3622666/71‑1 (MQ=255)
                                                            tctctcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGATGGGATTACGTTGGCGCTAAGcc         >  1:325106/1‑71 (MQ=255)
                                                            tctctcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGcc         >  1:3607785/1‑71 (MQ=255)
                                                            tctctcCATTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAAGGGATGATGTTGGCGCTAAGcc         >  1:2787581/1‑71 (MQ=255)
                                                            tctctcCATTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAAGGGATGATGTTGGCGCTAAGcc         >  1:1421941/1‑71 (MQ=255)
                                                                tcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGt                                     <  1:1315391/39‑1 (MQ=255)
                                                                tcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAg                               >  1:1611487/1‑45 (MQ=255)
                                                                tcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGccc        <  1:3926819/68‑1 (MQ=255)
                                                                tcCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCgg      >  1:3075498/1‑70 (MQ=255)
                                                                  cGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTg    <  1:3184306/70‑1 (MQ=255)
                                                                  cGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGc   <  1:4496159/71‑1 (MQ=255)
                                                                  cGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGc   <  1:1152506/71‑1 (MQ=255)
                                                                   gTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCg  <  1:3857659/71‑1 (MQ=255)
                                                                   gTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCg  <  1:1861470/71‑1 (MQ=255)
                                                                   |                                                                      
CAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCTCCGTTACGGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCG  >  minE/732803‑732940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: