| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 734,169 | C→T | 57.1% | P268L (CCG→CTG) | yceG → | predicted aminodeoxychorismate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 734,169 | 0 | C | T | 57.1% | 20.2 / 65.7 | 56 | P268L (CCG→CTG) | yceG | predicted aminodeoxychorismate lyase |
| Reads supporting (aligned to +/- strand): ref base C (10/14); new base T (19/13); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGAT > minE/734099‑734236 | gacccgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc < 1:690693/71‑1 (MQ=255) acccgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCg < 1:4587881/71‑1 (MQ=255) cgaccGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGa > 1:2829949/1‑69 (MQ=255) tGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGtat < 1:1273130/71‑1 (MQ=255) tGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGtat < 1:4698550/71‑1 (MQ=255) aTTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATaa > 1:2664537/1‑71 (MQ=255) tACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAacac > 1:3505327/1‑71 (MQ=255) tACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAacac > 1:1554344/1‑71 (MQ=255) tACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAaca < 1:3444783/70‑1 (MQ=255) aCGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAacac < 1:3356899/70‑1 (MQ=255) cGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACAcc < 1:4143050/70‑1 (MQ=255) cGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACAcc < 1:4501887/70‑1 (MQ=255) gATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCtata > 1:585861/1‑71 (MQ=255) tGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATAcc < 1:1040205/71‑1 (MQ=255) gCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCg > 1:908610/1‑71 (MQ=255) cGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGa < 1:4104734/43‑1 (MQ=255) cGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCgg < 1:4267289/71‑1 (MQ=255) gTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGt < 1:3383676/71‑1 (MQ=255) gTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCgg < 1:2715431/70‑1 (MQ=255) ttATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCg < 1:2514866/46‑1 (MQ=255) ttATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCta < 1:4022107/57‑1 (MQ=255) tataATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCAtt < 1:1285307/63‑1 (MQ=255) ataATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTg > 1:1547959/1‑71 (MQ=255) taATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCg < 1:3368786/43‑1 (MQ=255) taATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATaa > 1:2373207/1‑48 (MQ=255) taATGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTgc > 1:445463/1‑71 (MQ=255) aaTGGCAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATaa > 1:2268715/1‑47 (MQ=255) cAAACTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAaca < 1:706478/44‑1 (MQ=255) aaaCTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAgg > 1:3315368/1‑71 (MQ=255) aaaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGtat < 1:4249573/39‑1 (MQ=255) aaaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATaa > 1:4035512/1‑41 (MQ=255) aaaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAacac < 1:2498731/44‑1 (MQ=255) aaaCTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAgg > 1:1176816/1‑71 (MQ=255) aaCTTTCTCGTGCAGACCTGGAAACGCTGACAGCg < 1:2437303/35‑1 (MQ=255) aaCTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTc < 1:470655/60‑1 (MQ=255) aaCTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGt > 1:1531795/1‑71 (MQ=255) aCTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCta > 1:434898/1‑45 (MQ=255) aCTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGt > 1:966555/1‑70 (MQ=255) cTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCa > 1:758004/1‑66 (MQ=255) cTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGc < 1:3748249/71‑1 (MQ=255) cTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGc > 1:3632077/1‑71 (MQ=255) cTTTCTCGTGCAGACCTGGAAACGCTGACAGCGTATAACACATATACCATTACCGGTCTg > 1:2555848/1‑60 (MQ=255) cGTGCAGACCTGGAAACGCCGACAGCGTATAACACCta < 1:2020773/38‑1 (MQ=255) gTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAg < 1:1614360/69‑1 (MQ=255) gACCTGGAAACGCCGACAGCGTATAACACCTATAcc < 1:3471750/36‑1 (MQ=255) gACCTGGAAACGCCGACAGCGTATAACACCTATACCATTAGCGGTCTGCCGCCAGGTGCGATAGCGACGcc < 1:4379464/71‑1 (MQ=255) cTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAg > 1:545697/1‑52 (MQ=255) cTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAg > 1:4094906/1‑52 (MQ=255) cTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAg > 1:148166/1‑52 (MQ=255) cTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCggg > 1:1921048/1‑71 (MQ=255) cTGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCggg > 1:4021059/1‑71 (MQ=255) cTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCgc > 1:236956/1‑69 (MQ=255) tGGAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCg < 1:491585/56‑1 (MQ=255) ggAAACGCTGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCggggg > 1:2494966/1‑71 (MQ=255) aaaCGCCGACAGCGTATAACACCTATACCATTACCTGTCTGCCGCCAg > 1:2170813/1‑48 (MQ=255) cGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGAt > 1:4561714/1‑71 (MQ=255) gCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCgggg > 1:1422867/1‑64 (MQ=255) | GACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACACCTATACCATTACCGGTCTGCCGCCAGGTGCGATAGCGACGCCGGGGGCGGAT > minE/734099‑734236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |