| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 735,840 | C→T | 6.3% | A275V (GCC→GTC) | holB → | DNA polymerase III, delta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 735,840 | 0 | C | T | 6.3% | 246.3 / 4.6 | 79 | A275V (GCC→GTC) | holB | DNA polymerase III, delta prime subunit |
| Reads supporting (aligned to +/- strand): ref base C (41/33); new base T (2/3); total (43/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
ATGGATGCGCTAAAACGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTGCCACATTCGTG > minE/735773‑735908 | atggatgCGCTAAAACGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCg > 1:2983743/1‑70 (MQ=255)atggatgCGCTAAAACGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCg < 1:1582842/70‑1 (MQ=255)atggatgCGCTAAAACGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGa < 1:4656001/71‑1 (MQ=255)atggatgCGCTAAAACGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGa < 1:1736570/71‑1 (MQ=255) gatgCGCTAAAACGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAAc < 1:1268392/70‑1 (MQ=255) gatgCGCTAAAACGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACt > 1:3619217/1‑71 (MQ=255) gcTAAAACGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCaa < 1:2152927/71‑1 (MQ=255) tAAAACGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAAc > 1:4656103/1‑71 (MQ=255) aaaaCGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGa > 1:159144/1‑60 (MQ=255) aaaaCGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAAc > 1:1052261/1‑70 (MQ=255) aaaaCGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAAc > 1:4457117/1‑70 (MQ=255) aaaCGCCATTATGGCGCTGCGCACGTGACCAATGTTGATGTGCCGGGGCTGGTCGTCGAACTGGCAAAcc < 1:3175774/70‑1 (MQ=255) aaaCGCCATTATGGCGCTGCGCACGTGACCAATGTTGATGTGCCGGGGCTGGTCGTCGAACTGGCAAAcc < 1:606893/70‑1 (MQ=255) aaaCGCCATTATGGCGCTGCGCACGTGACCAATGTTGACGTGCCGGGGCTGGTCGTCGAACTGGCAAAcc < 1:633901/70‑1 (MQ=255) aaaCGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAAcc < 1:2744894/70‑1 (MQ=255) aaCGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCAt < 1:1939982/71‑1 (MQ=255) cGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCt > 1:4343890/1‑71 (MQ=255) cGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCt > 1:3151713/1‑71 (MQ=255) gCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAAc > 1:2899927/1‑57 (MQ=255) catcatGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTtc < 1:296016/71‑1 (MQ=255) catcatGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTtc < 1:3542979/71‑1 (MQ=255) catcatGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTtc < 1:2809933/71‑1 (MQ=255) atGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTccc > 1:346623/1‑71 (MQ=255) atGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTccc > 1:1975284/1‑71 (MQ=255) atGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTccc > 1:4293849/1‑71 (MQ=255) atGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTccc > 1:3039797/1‑71 (MQ=255) gTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGTACTGGCAAACCATCTTTCTCCCTcg > 1:497023/1‑71 (MQ=255) tgctgcGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTcgc < 1:3449387/71‑1 (MQ=255) gTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAAc > 1:3017146/1‑37 (MQ=255) gTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAAc > 1:2143419/1‑37 (MQ=255) gTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGc < 1:3903185/71‑1 (MQ=255) gTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGc < 1:304103/71‑1 (MQ=255) tGACCAATGTTGATGTGCCGGGGCTGGTCGTCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAgg > 1:2084325/1‑69 (MQ=255) tGACCAATGTTGATGTGCCGGGGCTGGTCGTCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAgg > 1:647897/1‑69 (MQ=255) aCCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCaaa > 1:3495557/1‑41 (MQ=255) aCCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCta > 1:2158308/1‑70 (MQ=255) ccAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCtata < 1:4153765/71‑1 (MQ=255) aaTGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACt < 1:334595/71‑1 (MQ=255) aaTGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACt < 1:970830/71‑1 (MQ=255) aTGTTGATGTGCCGTGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTcgcg < 1:1024932/55‑1 (MQ=255) aTGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCAt > 1:1015217/1‑41 (MQ=255) aTGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCAt > 1:2028352/1‑41 (MQ=255) aTGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTg < 1:2389435/71‑1 (MQ=255) aTGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTg > 1:594930/1‑71 (MQ=255) tGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCaaa < 1:263656/36‑1 (MQ=255) tGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCtat < 1:2016382/66‑1 (MQ=255) gTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGcc > 1:641820/1‑55 (MQ=255) ttGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCttt > 1:1464179/1‑42 (MQ=255) ttGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCta > 1:2203385/1‑48 (MQ=255) ttGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTc > 1:4223320/1‑49 (MQ=255) ttGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCgcgc > 1:4030272/1‑53 (MQ=255) ttGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTgggg > 1:1675258/1‑71 (MQ=255) ttGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTgggg < 1:1889239/71‑1 (MQ=255) ttGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTgggg > 1:4198250/1‑71 (MQ=255) ttGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTgggg > 1:2036966/1‑71 (MQ=255) ttGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTgggg > 1:36322/1‑71 (MQ=255) ttGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTgggg > 1:1069479/1‑71 (MQ=255) gATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATAc < 1:826098/64‑1 (MQ=255) gATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGa > 1:2578303/1‑71 (MQ=255) aTGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGAt > 1:3043978/1‑71 (MQ=255) tgtgCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATg < 1:3382121/71‑1 (MQ=255) tgtgCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATg < 1:743936/71‑1 (MQ=255) gtgCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCtat < 1:4332164/59‑1 (MQ=255) gtgCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTAGCGCCTGCAGGCTATACTGGGGGATGt > 1:1850522/1‑71 (MQ=255) tgCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGtt < 1:1802101/71‑1 (MQ=255) tgCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGtt < 1:779975/71‑1 (MQ=255) ccGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTg < 1:1269066/71‑1 (MQ=255) cGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTGc < 1:303606/71‑1 (MQ=255) ggCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGt > 1:549724/1‑65 (MQ=255) ggCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGt > 1:555092/1‑65 (MQ=255) ggCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTGCca < 1:489606/71‑1 (MQ=255) gTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTGCCACATTc < 1:3194654/70‑1 (MQ=255) gTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTGCCACATTc < 1:3020322/70‑1 (MQ=255) gTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTGCCACATTCg > 1:2843136/1‑71 (MQ=255) gTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTGCCACATTCg > 1:4633219/1‑71 (MQ=255) tCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCt > 1:4054341/1‑37 (MQ=255) tCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTGCCACATTCGt > 1:1867224/1‑71 (MQ=255) gCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTGCCACATTCGTg < 1:3707524/70‑1 (MQ=255) gCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTGCCACATTCGTg > 1:611599/1‑70 (MQ=255) ccGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATAc < 1:2781748/45‑1 (MQ=255) | ATGGATGCGCTAAAACGCCATCATGGTGCTGCGCAGGTGACCAATGTTGATGTGCCGGGCCTGGTCGCCGAACTGGCAAACCATCTTTCTCCCTCGCGCCTGCAGGCTATACTGGGGGATGTTTGCCACATTCGTG > minE/735773‑735908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |