| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 747,704 | T→C | 5.7% | P128P (CCA→CCG) | ycfQ ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 747,704 | 0 | T | C | 5.7% | 291.9 / 7.0 | 105 | P128P (CCA→CCG) | ycfQ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (45/54); new base C (4/2); total (49/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 108 total aligned reads displayed.
AAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGTTGG > minE/747634‑747774 | aaaaCTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCt < 1:2206851/71‑1 (MQ=255)aaaaCTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCt > 1:3325427/1‑71 (MQ=255) aaaCTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTg < 1:557803/71‑1 (MQ=255) aaaCTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTg < 1:3729514/71‑1 (MQ=255) aaCTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTgg > 1:4632430/1‑71 (MQ=255) aaCTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTg < 1:1152525/70‑1 (MQ=255) aaCTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTg < 1:2349170/70‑1 (MQ=255) ctgcAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGgaga < 1:565963/71‑1 (MQ=255) cAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCGACGTATTGGCGATATCTCCTGAATCTGGGGagag < 1:2535697/69‑1 (MQ=255) cAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGagag < 1:920181/69‑1 (MQ=255) cAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGagag > 1:2922484/1‑69 (MQ=255) cAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAggg < 1:339319/71‑1 (MQ=255) cAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAggg < 1:4686644/71‑1 (MQ=255) cAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAgg > 1:2938955/1‑70 (MQ=255) aaaGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTg < 1:1228676/61‑1 (MQ=255) aaGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGga < 1:3266996/64‑1 (MQ=255) aGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGggtgg < 1:443288/71‑1 (MQ=255) gTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGggtggt > 1:1843683/1‑71 (MQ=255) tGCGCTCTTGCATCGCATGGCGTGATTTCAGCGTATTGGCGATATCTCCTGAATCCTGGGAGAGGgtggtg < 1:2164710/71‑1 (MQ=255) gcgcTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg > 1:3910871/1‑70 (MQ=255) cgcTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg > 1:2530359/1‑69 (MQ=255) cgcTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg > 1:4699196/1‑69 (MQ=255) cgcTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCa < 1:2780581/71‑1 (MQ=255) cgcTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCa < 1:199255/71‑1 (MQ=255) cgcTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCATGAATCTGGGGAGAGGgtggtg > 1:3212016/1‑69 (MQ=255) ctctTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgt < 1:2803631/71‑1 (MQ=255) tctTGCATCGCATGGCGTGATTTCAGCGTATTGGCGATATCTCCTGAATCCGGGTAGAGggtgg > 1:4556252/1‑64 (MQ=255) tctTGCATCGCATGGCGTGATTTCAGCGTATTGGCGATATCTCCTGAATCCGGGGAGAGGGTGGTGCAgtt > 1:848126/1‑71 (MQ=255) tctTGCATCGCATGGCGTGATTTCAGCGTATTGGCGATATCTCCTGAATCCGGGGAGAGGGTGGTGCAgtt > 1:673948/1‑71 (MQ=255) tctTGCATCGCATGGCGTGATTTCAGCGTATTGGCGATATCTCCTGAATCCGGGGAGAGGGTGGTGCAgtt > 1:2882440/1‑71 (MQ=255) ttGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAggg > 1:353880/1‑59 (MQ=255) ttGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgtt < 1:1953277/69‑1 (MQ=255) ttGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgtt < 1:2988010/69‑1 (MQ=255) tGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTg > 1:1194687/1‑49 (MQ=255) tGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgttgtt > 1:2110245/1‑71 (MQ=255) gCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgttg < 1:3326688/68‑1 (MQ=255) cATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGttgttg < 1:2578958/70‑1 (MQ=255) tCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGgag < 1:2816656/50‑1 (MQ=255) cGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGga > 1:2352452/1‑48 (MQ=255) gCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgt > 1:4136880/1‑61 (MQ=255) gCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTTATCAt < 1:4127677/71‑1 (MQ=255) gCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCa > 1:2308839/1‑70 (MQ=255) gCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCAt > 1:462449/1‑71 (MQ=255) gCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCAt < 1:4306033/71‑1 (MQ=255) gCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCAt < 1:401755/71‑1 (MQ=255) tGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgt < 1:3609292/58‑1 (MQ=255) tGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGaa < 1:1865447/71‑1 (MQ=255) gCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAag < 1:2490396/70‑1 (MQ=255) cGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGgaga > 1:4607959/1‑43 (MQ=255) gATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGa > 1:641394/1‑58 (MQ=255) gATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGcc > 1:1137720/1‑71 (MQ=255) gATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGcc > 1:4238883/1‑71 (MQ=255) gATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGcc > 1:1190453/1‑71 (MQ=255) aTTTCAACGTATTGGCGATATCTCCTGAATCTGGGga > 1:4442934/1‑37 (MQ=255) tttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCGTg > 1:2836211/1‑71 (MQ=255) ttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCa > 1:851417/1‑47 (MQ=255) ttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGa > 1:4520544/1‑55 (MQ=255) ttCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAagc > 1:2026348/1‑64 (MQ=255) tCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCa > 1:4678184/1‑57 (MQ=255) tCAACGTATTGGCGATATCTCCTGAATCTCGGGAGAGGGTGGTGCAgttgt < 1:184012/51‑1 (MQ=255) cAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGa > 1:1569194/1‑53 (MQ=255) cAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGa > 1:3036876/1‑53 (MQ=255) cAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGcc < 1:3059828/70‑1 (MQ=255) aaCGTATTGGCGATATCTCCTGAATCTGGGGAGGGGGTGGTGCAGTTGTTGATCATGAagcagc > 1:1609525/1‑64 (MQ=255) tATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCa < 1:4128922/40‑1 (MQ=255) tATTGGCGATATCTCCTGAATCCGGGGAGAGGgtggtg < 1:3608227/38‑1 (MQ=255) aTTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCa < 1:1167420/39‑1 (MQ=255) aTTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCa > 1:2621694/1‑50 (MQ=255) aTTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCa > 1:272698/1‑50 (MQ=255) aTTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCa > 1:1564355/1‑50 (MQ=255) ttGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTc > 1:4456672/1‑71 (MQ=255) tGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCa > 1:2509393/1‑37 (MQ=255) cGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAagcagc > 1:860253/1‑54 (MQ=255) cGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGTGTGTCTTTg < 1:714137/71‑1 (MQ=255) cGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTg < 1:3807936/71‑1 (MQ=255) gATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGc < 1:4259459/71‑1 (MQ=255) gATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGc < 1:890567/71‑1 (MQ=255) atatCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGaa < 1:1163461/47‑1 (MQ=255) atatCTCATGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAag < 1:4494445/48‑1 (MQ=255) atCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGa < 1:2738148/38‑1 (MQ=255) tctcCTGAATCTGGGGAGAGGTTGGTGCAGTTGTTGATCATGa < 1:364094/43‑1 (MQ=255) tctcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGa < 1:1757333/43‑1 (MQ=255) tctcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTc < 1:2892868/62‑1 (MQ=255) tcCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTa > 1:128805/1‑70 (MQ=255) tcCTGAATCTGGGGAGAGCGTGGTGCAGTTGTTGATCATGaa > 1:1144939/1‑42 (MQ=255) ccTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCgg < 1:4650079/53‑1 (MQ=255) ccTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCgg < 1:1757100/53‑1 (MQ=255) cTGAATCTGGGGAGAGGGTTGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAg < 1:4697743/71‑1 (MQ=255) cTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAagcagc > 1:703953/1‑45 (MQ=255) cTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCt < 1:121393/47‑1 (MQ=255) cTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCt < 1:1337967/47‑1 (MQ=255) tGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAg < 1:1365209/70‑1 (MQ=255) gAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGa < 1:719482/37‑1 (MQ=255) gAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAagcag < 1:3432121/42‑1 (MQ=255) gAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGcc > 1:4393225/1‑48 (MQ=255) aaTCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAagcagc > 1:667473/1‑42 (MQ=255) aaTCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAagcagc > 1:2843896/1‑42 (MQ=255) aTCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGt < 1:4212377/71‑1 (MQ=255) aTCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGt < 1:4016690/71‑1 (MQ=255) cTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGTTg > 1:1339445/1‑71 (MQ=255) | AAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGTTGG > minE/747634‑747774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |