| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 894,163 | C→T | 49.0% | A88T (GCG→ACG) | sapB ← | predicted antimicrobial peptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 894,163 | 0 | C | T | 49.0% | 7.5 / 144.4 | 102 | A88T (GCG→ACG) | sapB | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (27/25); new base T (22/28); total (49/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
AAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCG > minE/894096‑894231 | aaGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTaa > 1:4110294/1‑70 (MQ=255)aaGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTaa > 1:414031/1‑70 (MQ=255)aaGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCaa < 1:2470509/70‑1 (MQ=255) gTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAAc > 1:4247872/1‑71 (MQ=255) ttATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAAcc < 1:4000247/71‑1 (MQ=255) ttATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAAcc < 1:1922759/71‑1 (MQ=255) ttATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAAcc < 1:2489794/71‑1 (MQ=255) ttATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAAcc < 1:191499/71‑1 (MQ=255) ttATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCCCatcg < 1:2785539/69‑4 (MQ=255) tATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAAcc > 1:3398239/1‑70 (MQ=255) tATCCTGCCACTTATGGCGCGTAAACCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCCCatcg < 1:3758185/68‑4 (MQ=255) aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCg < 1:3525114/70‑1 (MQ=255) aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCg < 1:3530522/70‑1 (MQ=255) aTCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCg < 1:2336689/70‑1 (MQ=255) ccTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAg > 1:422845/1‑71 (MQ=255) ccACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCg < 1:720430/64‑1 (MQ=255) ccACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGc < 1:907327/69‑1 (MQ=255) cACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAg < 1:4320638/71‑1 (MQ=255) aCTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAg < 1:1040865/70‑1 (MQ=255) cTTATGGCGCGTAATCCCTGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGa < 1:2479451/71‑1 (MQ=255) cTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAg < 1:3874820/69‑1 (MQ=255) cTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGa < 1:1816766/71‑1 (MQ=255) ttATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGTGAGGAt > 1:4171861/1‑71 (MQ=255) tATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg > 1:4260959/1‑71 (MQ=255) aTGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg > 1:3442116/1‑70 (MQ=255) gcgcGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGcaca < 1:1372980/71‑1 (MQ=255) gcgcGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGcaca < 1:11934/71‑1 (MQ=255) cgcgTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAg < 1:820275/71‑1 (MQ=255) cgTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGtt < 1:1830411/71‑1 (MQ=255) tAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTcc < 1:3647064/71‑1 (MQ=255) tAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTcc < 1:948388/71‑1 (MQ=255) aTCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:4205774/1‑71 (MQ=255) aTCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:3860693/1‑71 (MQ=255) aTCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:172556/1‑71 (MQ=255) aTCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCg < 1:4634210/48‑1 (MQ=255) aTCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAg > 1:1199477/1‑56 (MQ=255) tCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCAtc < 1:4284635/71‑1 (MQ=255) ccGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCAtcgt > 1:2559257/1‑71 (MQ=255) ccGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCAtcg < 1:2687230/70‑1 (MQ=255) cGGCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGaa < 1:3229486/46‑1 (MQ=255) ggCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCAtcgtcg > 1:1754645/1‑71 (MQ=255) gCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAAcc < 1:3865571/41‑1 (MQ=255) gCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAg < 1:3854539/50‑1 (MQ=255) gCAATCATCCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAg < 1:114437/50‑1 (MQ=255) aaTCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg < 1:4175406/71‑1 (MQ=255) aaTCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:3329252/1‑71 (MQ=255) atcatcCCCACGGGGATGCCAACAATCAGCGTAAAACCg < 1:1091519/39‑1 (MQ=255) atcatcCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAg < 1:1900479/47‑1 (MQ=255) atcatcCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGca < 1:3891798/53‑1 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCggg > 1:3735932/1‑70 (MQ=255) catcCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCg > 1:4416975/1‑68 (MQ=255) atcCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCggg > 1:1346054/1‑69 (MQ=255) tcCCCACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGcc < 1:4236049/65‑1 (MQ=255) ccccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGa > 1:3268836/1‑71 (MQ=255) ccccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGa > 1:1057593/1‑71 (MQ=255) cccACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGaa > 1:3887829/1‑68 (MQ=255) cccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATg > 1:2873538/1‑45 (MQ=255) cccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCAtc > 1:594414/1‑57 (MQ=255) cccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGa > 1:1045520/1‑70 (MQ=255) cccACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGAc > 1:1230709/1‑71 (MQ=255) cACGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGaa < 1:2455753/66‑1 (MQ=255) aCGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAg > 1:1539669/1‑47 (MQ=255) aCGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACctc > 1:4437066/1‑71 (MQ=255) aCGGGGATGCCAACAATCAGAGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACctc > 1:2858348/1‑71 (MQ=255) cGGGGATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACctct > 1:4554262/1‑71 (MQ=255) cGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGc > 1:1293607/1‑58 (MQ=255) ggggATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAg < 1:2137241/64‑1 (MQ=255) ggggATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCtt > 1:2237324/1‑71 (MQ=255) gggATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCAtcgt > 1:4600159/1‑53 (MQ=255) gggATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCAtcgt > 1:2679478/1‑53 (MQ=255) gggATGCCAACAATCAGCGCAAAACCGAAGGCGAg < 1:2934539/35‑1 (MQ=255) gggATGCCAACAATCAGCGCAAAACCGAAGGCGAg < 1:4028823/35‑1 (MQ=255) gggATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCtt > 1:2992384/1‑70 (MQ=255) ggATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCtttt < 1:2710929/71‑1 (MQ=255) ggATGCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCtttt < 1:2308541/71‑1 (MQ=255) ggATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCtttt < 1:2082085/71‑1 (MQ=255) aTGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTaa < 1:2550779/71‑1 (MQ=255) tGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAAc < 1:2456857/71‑1 (MQ=255) gCCAACAATCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACt > 1:2472086/1‑71 (MQ=255) gCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACt > 1:3606880/1‑71 (MQ=255) aacaaTCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCggg < 1:4142661/52‑1 (MQ=255) aacaaTCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAAc > 1:3373568/1‑67 (MQ=255) acaaTCAGCGTAAAACCGAAGGCGAGGATGCACAGTTcc < 1:1791623/39‑1 (MQ=255) acaaTCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACt > 1:1155931/1‑67 (MQ=255) caaTCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCg > 1:1888281/1‑71 (MQ=255) caaTCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCg > 1:308267/1‑71 (MQ=255) aaTCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGaa > 1:4193730/1‑51 (MQ=255) aaTCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCgg > 1:445381/1‑71 (MQ=255) aaTCAGCGCAAAACCGAAGGCGAAGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTaa > 1:3636717/1‑63 (MQ=255) aTCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGc < 1:1641259/71‑1 (MQ=255) tCAGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCtttt < 1:2935650/59‑1 (MQ=255) tCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAg < 1:3050396/50‑1 (MQ=255) cAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGaa > 1:2479246/1‑48 (MQ=255) cAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGaa > 1:2762161/1‑48 (MQ=255) aGCGTAAAACCGAAGGCGAGGATGCACAGTTCCAt > 1:3153213/1‑35 (MQ=255) aGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCggg < 1:1543485/45‑1 (MQ=255) aGCGTAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAAt > 1:4130247/1‑71 (MQ=255) gcgcAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATc < 1:4300900/71‑1 (MQ=255) gcgaAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACctct < 1:3955644/53‑1 (MQ=255) cgcAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCg < 1:3048898/71‑1 (MQ=255) | AAGTTATCCTGCCACTTATGGCGCGTAATCCCGGCAATCATCCCCACGGGGATGCCAACAATCAGCGCAAAACCGAAGGCGAGGATGCACAGTTCCATCGTCGCCGGGAAGACCTCTTTTAACTGTTCGGCAATCG > minE/894096‑894231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |