| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 931,499 | C→T | 5.4% | A239A (GCG→GCA) | yddG ← | predicted methyl viologen efflux pump |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 931,499 | 0 | C | T | 5.4% | 291.1 / 4.2 | 93 | A239A (GCG→GCA) | yddG | predicted methyl viologen efflux pump |
| Reads supporting (aligned to +/- strand): ref base C (42/45); new base T (3/2); total (46/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
GCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACC > minE/931430‑931567 | gccgcAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAgcg > 1:4548283/1‑71 (MQ=255) ccgcAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAgcgc > 1:3709472/1‑71 (MQ=255) cgcAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGTGGCGCTGAGCAGCACGGCTGCAAGCGCt < 1:4088489/71‑1 (MQ=255) cgcAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCt < 1:3046442/71‑1 (MQ=255) cgcAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCt < 1:3331430/71‑1 (MQ=255) gcAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCt > 1:2023815/1‑70 (MQ=255) cAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCt > 1:1457810/1‑69 (MQ=255) ccATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGa < 1:929439/70‑1 (MQ=255) tCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAg < 1:4154415/70‑1 (MQ=255) cAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGt > 1:1652314/1‑70 (MQ=255) aGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTa > 1:1093756/1‑70 (MQ=255) aGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTa > 1:4579910/1‑70 (MQ=255) aGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTa < 1:466854/70‑1 (MQ=255) aGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTa > 1:3185868/1‑70 (MQ=255) aGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTa < 1:911771/70‑1 (MQ=255) gcgcCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTa > 1:381432/1‑67 (MQ=255) gcgcCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAgg > 1:2855293/1‑71 (MQ=255) gcgcCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAgg > 1:3609003/1‑71 (MQ=255) gcCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCg > 1:187950/1‑71 (MQ=255) ccTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCg < 1:4199814/70‑1 (MQ=255) cTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCGCGGCTGCAAGCGCTGAGGAAAGTACAGGCGt > 1:707070/1‑70 (MQ=255) cTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGt < 1:2949006/62‑1 (MQ=255) cTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGt < 1:3839302/62‑1 (MQ=255) cTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTa < 1:3738026/71‑1 (MQ=255) ccAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTaaaa > 1:3878668/1‑70 (MQ=255) ccAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTaaaa < 1:2548261/70‑1 (MQ=255) ccAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTaaaa < 1:3316984/70‑1 (MQ=255) ccAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAAt < 1:3594256/71‑1 (MQ=255) cAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATa > 1:1803735/1‑71 (MQ=255) cAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATa < 1:728425/71‑1 (MQ=255) cAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATa < 1:308792/71‑1 (MQ=255) agaacgagaacgACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGa > 1:2484692/1‑49 (MQ=255) agaacgagaacgACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATAc > 1:2294263/1‑71 (MQ=255) agaacgagaacgACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATAc > 1:195681/1‑71 (MQ=255) agaacgagaacgACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATAc > 1:3429517/1‑71 (MQ=255) aacgagaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACg > 1:4494013/1‑70 (MQ=255) aacgagaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACg > 1:2420407/1‑70 (MQ=255) aacgagaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACg > 1:1308068/1‑70 (MQ=255) aaagagaacgaCAGCGGGGCGCTGAGCAGCAGGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGa < 1:3502674/68‑1 (MQ=255) acgagaacgaCAGCGGGTCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGa < 1:2310336/70‑1 (MQ=255) acgagaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAg > 1:296966/1‑47 (MQ=255) acgagaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTa < 1:3623272/54‑1 (MQ=255) acgagaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGaa > 1:2541770/1‑71 (MQ=255) acgagaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGa < 1:2315796/70‑1 (MQ=255) gagaacgaCAGCGGGGCGCTGAGTAACACGGCTGCAAGTGCTGAGGAAAGGACGGGCGTaaaa < 1:2456821/63‑1 (MQ=255) gagaacgaCAGCGGGGCGCTGAGTAACACGGCTGCAAGTGCTGAGGAAAGGACGGGCGTaaaa < 1:2003876/63‑1 (MQ=255) gagaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAAcc < 1:2072365/71‑1 (MQ=255) agaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCt < 1:1997467/71‑1 (MQ=255) gaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGaaa > 1:2585757/1‑47 (MQ=255) gaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCt < 1:2762331/70‑1 (MQ=255) acgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGa < 1:1026630/43‑1 (MQ=255) cgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGaa > 1:1115834/1‑64 (MQ=255) gaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTaa < 1:3408388/55‑1 (MQ=255) gCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACg < 1:139828/57‑1 (MQ=255) cGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATaa < 1:299672/71‑1 (MQ=255) ggggCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAAt > 1:2723826/1‑71 (MQ=255) ggggCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAAt > 1:1854054/1‑71 (MQ=255) gcTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGa < 1:2848767/71‑1 (MQ=255) gAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTaaaa < 1:1391681/43‑1 (MQ=255) agcagcACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTaaaa > 1:4393610/1‑42 (MQ=255) cagcaCGGCTGCAAGCGCTGAGGAAAGTACAGGCGTaa < 1:2964033/38‑1 (MQ=255) agcaCGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACAt < 1:1153927/67‑1 (MQ=255) gcaCGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGa < 1:2570046/63‑1 (MQ=255) gcaCGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGa < 1:1078188/63‑1 (MQ=255) gcaCGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGa < 1:3616308/63‑1 (MQ=255) aCGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGt > 1:3383940/1‑59 (MQ=255) aCGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGt > 1:1039202/1‑59 (MQ=255) aCGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCacg > 1:3632096/1‑69 (MQ=255) aCGCCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATg < 1:938935/71‑1 (MQ=255) cGGCTGCAAGCGCTGAGGAAAGTACAGGCGTaaaa > 1:1552703/1‑35 (MQ=255) cGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGc < 1:3228335/71‑1 (MQ=255) cGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGc < 1:2423196/71‑1 (MQ=255) cGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGc < 1:4318387/71‑1 (MQ=255) ggCTGCAAGTGCTGAGGAAAGGACGGGCGTAAAATACGaa > 1:177766/1‑40 (MQ=255) ggCTGCAAGTGCTGAGGAAAGGACGGGCGTAAAATACGaa > 1:219954/1‑40 (MQ=255) ggCTGCAAGTGCTGAGGAAAGGACGGGCGTAAAATACGaa > 1:4248562/1‑40 (MQ=255) ggCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGc > 1:705990/1‑70 (MQ=255) ggCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGc > 1:279164/1‑70 (MQ=255) ggCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGc > 1:2225048/1‑70 (MQ=255) ggCTGCAAGAGCTGAGGAAAGGACGGGCGTAAAATACGaa > 1:4128742/1‑40 (MQ=255) gCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGa > 1:4405791/1‑38 (MQ=255) gCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTTACATTGCCATGCaa < 1:2762955/71‑1 (MQ=255) gCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCaa < 1:3859284/71‑1 (MQ=255) gCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCaa < 1:3796210/71‑1 (MQ=255) cAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATaa > 1:3377123/1‑48 (MQ=255) cAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCaa < 1:2984253/67‑1 (MQ=255) cAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAatat < 1:279496/70‑1 (MQ=255) aaGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCAt < 1:3045214/45‑1 (MQ=255) aGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTa > 1:640460/1‑37 (MQ=255) aGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTa > 1:1029124/1‑37 (MQ=255) aGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACAtt > 1:4270215/1‑56 (MQ=255) aGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACAtt > 1:1049475/1‑56 (MQ=255) aGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAata > 1:3148085/1‑67 (MQ=255) aGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAata > 1:1725198/1‑67 (MQ=255) aGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATAcc < 1:1945295/71‑1 (MQ=255) gcgcTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATAcc > 1:1259480/1‑70 (MQ=255) | GCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACC > minE/931430‑931567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |