| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 139,441 | C→T | 5.6% | S242S (TCG→TCA) | htrE ← | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 139,441 | 0 | C | T | 5.6% | 331.6 / 6.3 | 106 | S242S (TCG→TCA) | htrE | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base C (56/44); new base T (3/3); total (59/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
Only 100 of 112 total aligned reads displayed.
TCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGT > minE/139372‑139511 | tCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGc > 1:1237731/1‑70 (MQ=255)tCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGc > 1:586030/1‑70 (MQ=255) cGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGc > 1:4009284/1‑69 (MQ=255) ggATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCg < 1:356709/69‑1 (MQ=255) ggATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCg < 1:1133574/69‑1 (MQ=255) ggATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAg > 1:4461418/1‑71 (MQ=255) gATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAgg > 1:4123446/1‑71 (MQ=255) tACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAgg > 1:619415/1‑69 (MQ=255) tACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGc < 1:4586373/70‑1 (MQ=255) cTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAg > 1:2498632/1‑66 (MQ=255) cTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGa < 1:3140726/70‑1 (MQ=255) cTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGa > 1:445450/1‑70 (MQ=255) gACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGa < 1:119442/68‑1 (MQ=255) gAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCa > 1:3166327/1‑69 (MQ=255) gAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg < 1:2577496/71‑1 (MQ=255) tCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGc < 1:2286835/59‑1 (MQ=255) cAAAGGTTTCGCCCGTCGTATAAGTCTAACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTg > 1:3542944/1‑70 (MQ=255) aaaGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGc > 1:1538598/1‑57 (MQ=255) aaaGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGaa < 1:1160348/71‑1 (MQ=255) aaaGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGaa > 1:3697075/1‑71 (MQ=255) aaaGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGaa < 1:2124840/71‑1 (MQ=255) aGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGTGAGGCGATATCACGCTGAACa < 1:1219375/71‑1 (MQ=255) aGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACa < 1:2988587/71‑1 (MQ=255) gTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATa > 1:3195582/1‑71 (MQ=255) tttCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATa < 1:4104631/70‑1 (MQ=255) tttCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATAc < 1:2744829/71‑1 (MQ=255) tttCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATAc < 1:4673426/71‑1 (MQ=255) ttCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGat > 1:3415044/1‑54 (MQ=255) ttCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATAcc > 1:1650237/1‑71 (MQ=255) ttCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATAcc > 1:1679904/1‑71 (MQ=255) ttCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATAcc > 1:3351192/1‑71 (MQ=255) tCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg < 1:259991/59‑1 (MQ=255) tCGCCCCTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACa > 1:604516/1‑66 (MQ=255) cgtcgtATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCt > 1:1788105/1‑71 (MQ=255) tcgtATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCt > 1:793084/1‑69 (MQ=255) gtATAAGACTCACCAAGAATGAGTTGAGAACGCAGTGAGGCGa > 1:4018680/1‑43 (MQ=255) tataAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCAc < 1:1178595/48‑1 (MQ=255) ataAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGaa < 1:181433/53‑1 (MQ=255) taAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTg < 1:2017695/50‑1 (MQ=255) taAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAAt > 1:2748206/1‑70 (MQ=255) taAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAAt > 1:160002/1‑70 (MQ=255) taAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAAt < 1:3714228/70‑1 (MQ=255) aGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGaa > 1:264332/1‑50 (MQ=255) aGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATa > 1:2957269/1‑54 (MQ=255) aGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATAcc > 1:1328563/1‑56 (MQ=255) aGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCa > 1:3360763/1‑70 (MQ=255) aGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCa > 1:4007521/1‑70 (MQ=255) aGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCa > 1:3978921/1‑70 (MQ=255) aGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCa > 1:1448117/1‑70 (MQ=255) aGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCa > 1:3573198/1‑70 (MQ=255) cTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGaa > 1:3730581/1‑47 (MQ=255) cTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATc < 1:1690020/66‑1 (MQ=255) cTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAAt < 1:1111016/71‑1 (MQ=255) tCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCt < 1:3487763/43‑1 (MQ=255) tCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCAt < 1:3879262/67‑1 (MQ=255) aCCAAGAATGAGTTGAGAACGCAGTGAGGCGATATCa > 1:437793/1‑37 (MQ=255) aCCAAGAATGAGTTGAGAACGCAGTGAGGCGATATCa > 1:2161301/1‑37 (MQ=255) aCCAAGAATGAGTTGAGAACGCAGTGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTAc < 1:2324815/71‑1 (MQ=255) aCCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATaa > 1:717911/1‑67 (MQ=255) aCCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATa < 1:3057123/66‑1 (MQ=255) ccAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACt > 1:2477554/1‑71 (MQ=255) cAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATa < 1:4515200/64‑1 (MQ=255) aaGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATaa > 1:1602160/1‑64 (MQ=255) aaGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGc > 1:4406402/1‑71 (MQ=255) aaGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGc > 1:1539883/1‑71 (MQ=255) aaGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGc > 1:87516/1‑71 (MQ=255) aGAATGAGTTGAGAACGCAGCGAGGCGATATCCCGCTGAACATACCGATTCTTaaaa > 1:2784219/1‑57 (MQ=255) aGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGaa < 1:3074589/40‑1 (MQ=255) aGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATaa > 1:4294300/1‑63 (MQ=255) aGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATaa > 1:2883953/1‑63 (MQ=255) aaTGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGc > 1:4263819/1‑68 (MQ=255) aTGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATc < 1:1777074/56‑1 (MQ=255) aTGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAg < 1:3285887/70‑1 (MQ=255) aTGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAg < 1:1968969/70‑1 (MQ=255) aTGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGa < 1:2444346/71‑1 (MQ=255) tGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCg > 1:3109231/1‑43 (MQ=255) gTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCg < 1:2080839/71‑1 (MQ=255) gTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCg < 1:2460008/71‑1 (MQ=255) ttGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATc < 1:1029496/51‑1 (MQ=255) ttGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCgg < 1:1844680/71‑1 (MQ=255) gagaACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATaa > 1:2459663/1‑53 (MQ=255) gagaACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATaa > 1:3478955/1‑53 (MQ=255) gagaACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGt > 1:2578394/1‑70 (MQ=255) gagaACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGt > 1:2738068/1‑70 (MQ=255) gagaACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGt > 1:2743490/1‑70 (MQ=255) gagaACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGt < 1:1819094/70‑1 (MQ=255) agaACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCa > 1:3748594/1‑71 (MQ=255) gaACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCAt > 1:1088009/1‑71 (MQ=255) aaCGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAg < 1:1094865/60‑1 (MQ=255) aaCGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAg < 1:1010179/60‑1 (MQ=255) cGCAGCGAGGCGATATCACGCTGAACATACCGATTCt > 1:2935916/1‑37 (MQ=255) cGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAAt > 1:2734994/1‑49 (MQ=255) cGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAAt > 1:3548156/1‑49 (MQ=255) gCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTa > 1:2885949/1‑50 (MQ=255) aGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGtt > 1:2670022/1‑71 (MQ=255) gCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAAt < 1:1856230/45‑1 (MQ=255) gCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCTGTCATCCAGt > 1:4529828/1‑69 (MQ=255) cGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGt < 1:4499533/71‑1 (MQ=255) cGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGt < 1:632906/71‑1 (MQ=255) cGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGt < 1:2270619/71‑1 (MQ=255) | TCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGT > minE/139372‑139511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |