| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,026,282 | C→A | 6.5% | R197L (CGT→CTT) ‡ | malI ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,026,282 | 0 | C | A | 6.5% | 288.2 / 3.0 | 91 | R197L (CGT→CTT) ‡ | malI | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (37/48); new base A (4/2); total (41/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
CAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGC > minE/1026216‑1026347 | cAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGt > 1:1864089/1‑71 (MQ=255)cAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGt > 1:487951/1‑71 (MQ=255) cTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAAt < 1:1355247/69‑1 (MQ=255) aTGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAg > 1:1974277/1‑70 (MQ=255) tGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAgg > 1:407766/1‑70 (MQ=255) aaaCGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGt > 1:3535286/1‑60 (MQ=255) aaaCGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAAt > 1:1074950/1‑64 (MQ=255) aaaCGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGaa < 1:731033/70‑1 (MQ=255) aaaCGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAAc < 1:664021/71‑1 (MQ=255) aaaCGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAAc < 1:3346185/71‑1 (MQ=255) aaaCGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAAc > 1:3959190/1‑71 (MQ=255) actgCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACtt > 1:3860929/4‑71 (MQ=255) cGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACggg > 1:800629/1‑56 (MQ=255) cGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACttt > 1:1507395/1‑71 (MQ=255) cGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACttt > 1:2320379/1‑71 (MQ=255) cGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCAAACCCGTTCTGCACGGGTTAATGAGGAACttt > 1:545630/1‑71 (MQ=255) ggCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTg < 1:3133852/71‑1 (MQ=255) ggCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTg < 1:1143929/71‑1 (MQ=255) ggCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTg < 1:4351545/71‑1 (MQ=255) gCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGc < 1:4486738/71‑1 (MQ=255) cAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGGTAATGAGGAACTTTGcc > 1:3935471/1‑71 (MQ=255) gCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTc < 1:3675724/71‑1 (MQ=255) gCCAAATTTTAGTAGAGTCGCACAATAGCCCCCCACCCGTTCTGCCAGGGTTAAt < 1:2486913/55‑1 (MQ=255) gCCAAATTTTAGTAGAGTCGCACAATAGCCCCCCACCCGTTCTGCCAGGGTTAAt < 1:2481311/55‑1 (MQ=255) ccAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTcc > 1:2620407/1‑71 (MQ=255) cAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTccc < 1:4147251/71‑1 (MQ=255) cAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTcc > 1:404403/1‑70 (MQ=255) aaTTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAgg < 1:320617/55‑1 (MQ=255) aaTTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAgg < 1:1467789/55‑1 (MQ=255) aTTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGATGAACTTTGCCCTCccagc < 1:2925210/71‑1 (MQ=255) aTTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccagc < 1:3876115/71‑1 (MQ=255) aTTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccagc < 1:3498971/71‑1 (MQ=255) tagAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGa < 1:1224232/71‑1 (MQ=255) tagAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGa < 1:3192031/71‑1 (MQ=255) gagTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATc > 1:3726274/1‑71 (MQ=255) tGCACAATAGCCCCCAAGCCGTTCTGCACGGGTTAATGAGGaa < 1:2491385/43‑1 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt > 1:3836022/1‑70 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt > 1:1746715/1‑70 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt > 1:1547410/1‑70 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:3480297/70‑1 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:3031291/70‑1 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:4476218/70‑1 (MQ=255) gCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:2430290/70‑1 (MQ=255) cacaATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:4178244/69‑1 (MQ=255) acaATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGAt < 1:2698070/71‑1 (MQ=255) caATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTc < 1:3155229/49‑1 (MQ=255) caATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATg > 1:4210805/1‑71 (MQ=255) aaTAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:2956926/66‑1 (MQ=255) aTAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGcc < 1:3759363/71‑1 (MQ=255) tAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGa > 1:4620860/1‑66 (MQ=255) aGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCa > 1:2711161/1‑71 (MQ=255) gCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCAt > 1:1743139/1‑71 (MQ=255) gCCCCCAACCCGTTCTGCACGGGTAAATGAGGAACTTTGccc > 1:2004074/1‑42 (MQ=255) cccAACCCGTTCTGCACGGGTTAATGAGGAACttt < 1:757539/35‑1 (MQ=255) cccAACCCGTTCTGCACGGGTTAATGAGGAACttt < 1:1413542/35‑1 (MQ=255) cccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:2309540/59‑1 (MQ=255) cccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:4602983/59‑1 (MQ=255) cccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTg < 1:4460691/60‑1 (MQ=255) cccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCAtttc < 1:2293929/71‑3 (MQ=255) ccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:529317/58‑1 (MQ=255) ccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCg < 1:2748201/71‑1 (MQ=255) ccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCg < 1:3430805/71‑1 (MQ=255) ccAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCg < 1:3883145/71‑1 (MQ=255) ccaCCCGTTCTGCCAGGGTTAATGAGGAACTTTGTCCTCCCAGCCAGGCGATACGCTGATGCCCATTGCg > 1:3033247/3‑70 (MQ=255) cAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATc < 1:1416749/53‑1 (MQ=255) cAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCg > 1:2843065/1‑70 (MQ=255) aaCCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:6938/56‑1 (MQ=255) aaCCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:2210166/56‑1 (MQ=255) aaCCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCa > 1:4238012/1‑64 (MQ=255) aaCCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCg > 1:1451187/1‑69 (MQ=255) aaCCCGTTCTGCACGGGTTAATGAGGAACTTTGCACTCCCAGCCAGGCGAt > 1:3673000/1‑51 (MQ=255) aCCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccagc < 1:4091481/42‑1 (MQ=255) cccGTTCTGCCAGGGTTAATGAGGAACTTTGTCCTCCCAGCCAGGCGATACGCTGATGCCCATTGCGAAt > 1:892211/1‑70 (MQ=255) cccGTTCTGCCAGGGTTAATGAGGAACTTTGTCCTCCCAGCCAGGCGATACGCTGATGCCCATTGCGAAt > 1:2814333/1‑70 (MQ=255) cccGTTCTGCCAGGGTTAATGAGGAACTTTGTCCTCCCAGCCAGGCGATACGCTGATGCCCATTGCGAAt > 1:2800439/1‑70 (MQ=255) cccGTTCTGCACGGGTTAATGAGGAACTTTGGCCTCccagccag < 1:292527/44‑1 (MQ=255) cccGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCccagccat < 1:1157882/44‑2 (MQ=255) cccGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGAt > 1:455471/1‑49 (MQ=255) ccGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAAt > 1:1819795/1‑69 (MQ=255) ccGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAAt > 1:3622447/1‑69 (MQ=255) ccGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATga < 1:2103489/71‑1 (MQ=255) cGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGc < 1:1242914/57‑1 (MQ=255) cGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAAt > 1:4363192/1‑68 (MQ=255) ttCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCt < 1:2134950/50‑1 (MQ=255) ttCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGcc < 1:1232607/56‑1 (MQ=255) ttCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCAt > 1:3504996/1‑59 (MQ=255) ttCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGACCAt > 1:2591618/1‑59 (MQ=255) ttCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCAAGGCGATCCGCt < 1:3488346/50‑1 (MQ=255) cTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATc > 1:3011095/1‑44 (MQ=255) cTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATgag > 1:3230069/1‑67 (MQ=255) cTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGc > 1:1800747/1‑71 (MQ=255) | CAATCGCTATGAAACGGCAGGCCAAATTTTAGTAGAGTTGCACAATAGCCCCCAACCCGTTCTGCACGGGTTAATGAGGAACTTTGCCCTCCCAGCCAGGCGATCCGCTGATGCCCATTGCGAATGAGATGC > minE/1026216‑1026347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |