| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,118,451 | G→A | 6.0% | A162V (GCG→GTG) | ydiU ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,118,451 | 0 | G | A | 6.0% | 203.1 / 2.5 | 66 | A162V (GCG→GTG) | ydiU | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (32/30); new base A (2/2); total (34/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 | |||||||||||
AAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGAT > minE/1118383‑1118520 | aaaaaTGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccgcc > 1:2341774/1‑71 (MQ=255) aaaaTGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccgccg > 1:934468/1‑71 (MQ=255) aaaaTGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccgccg > 1:2702688/1‑71 (MQ=255) aaaaTGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccgccg > 1:849264/1‑71 (MQ=255) aaaTGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGt < 1:4438858/71‑1 (MQ=255) aaTGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGtt > 1:4217380/1‑71 (MQ=255) tGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTc > 1:2794939/1‑71 (MQ=255) ttCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTccc < 1:2121411/71‑1 (MQ=255) cGAAATTACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCg < 1:2178618/70‑1 (MQ=255) cGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCg < 1:1764701/70‑1 (MQ=255) cGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGa > 1:1689918/1‑71 (MQ=255) cGAAATGACCAAAGCGCAGATGACTTGGTGCAACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGa > 1:3379215/1‑71 (MQ=255) aaaTGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATa > 1:2361756/1‑71 (MQ=255) aCCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTg < 1:3155759/71‑1 (MQ=255) aaaGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTccc < 1:4083480/59‑1 (MQ=255) cgcAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCTTTTCCCGATACACTGGCGAATc < 1:2357817/71‑1 (MQ=255) cAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCg < 1:2794847/70‑1 (MQ=255) aGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTcc > 1:1650615/1‑51 (MQ=255) tGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATaca > 1:2616093/1‑55 (MQ=255) gACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTcc > 1:3202578/1‑47 (MQ=255) gACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGa > 1:4550111/1‑50 (MQ=255) gACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGt > 1:3649497/1‑70 (MQ=255) gACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGt > 1:562364/1‑70 (MQ=255) gACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGt > 1:2817458/1‑70 (MQ=255) gACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGt > 1:1691475/1‑70 (MQ=255) gACTTGGTGCCACACGCATCAGCATCGCGCCTGACTCCACCGTTTCCCGATACACTGGCGAATCGCTGGt < 1:714957/70‑1 (MQ=255) aCTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTg < 1:4536341/70‑1 (MQ=255) aCTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTg < 1:1694466/70‑1 (MQ=255) aCTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGa < 1:280709/71‑1 (MQ=255) aCTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGa < 1:4274794/71‑1 (MQ=255) cTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTg < 1:4316446/69‑1 (MQ=255) cTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGAc < 1:139018/71‑1 (MQ=255) tGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGa > 1:3008774/1‑71 (MQ=255) ggTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGa > 1:348860/1‑70 (MQ=255) gTGCCACACGCATCAGCATCGCGCCTGACTCCACCGTTTCCCGATACACTGGCGAATCGCTGGTGACGAt < 1:3725507/70‑1 (MQ=255) ccACACGCATCAGCATCTCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACtt < 1:3090433/71‑1 (MQ=255) acacGCATCAGCATCGCGCCTGACTCCACCGTTTCCCGATACACTGGCGAATCGc > 1:2334425/1‑55 (MQ=255) acacGCATCAGCATCGCGCCTGACTCCACCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTa > 1:753958/1‑70 (MQ=255) acGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcg > 1:3109144/1‑71 (MQ=255) cGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGc > 1:2168606/1‑45 (MQ=255) cGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATAc > 1:1435511/1‑64 (MQ=255) cGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgc < 1:2171750/71‑1 (MQ=255) cGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgc < 1:1846979/71‑1 (MQ=255) cGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgc > 1:4545402/1‑71 (MQ=255) cGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgc < 1:1617045/71‑1 (MQ=255) gCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgcg > 1:709789/1‑71 (MQ=255) gCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgcg < 1:4379989/71‑1 (MQ=255) gCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgcg > 1:1716141/1‑71 (MQ=255) cATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgcgc < 1:4565444/71‑1 (MQ=255) cATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAcgcg < 1:3078624/70‑1 (MQ=255) cAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGc < 1:3693050/47‑1 (MQ=255) cAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAAc > 1:3690947/1‑64 (MQ=255) aGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGTTGGTGACGATACTTAACGCGCggg > 1:4446537/1‑70 (MQ=255) aGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTaa < 1:306003/62‑1 (MQ=255) aGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGgt > 1:2724991/1‑71 (MQ=255) tCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGgtcgt > 1:4296942/1‑70 (MQ=255) gcgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTTACGATACTTAACGCGCGGGTCGTCgg < 1:1426267/71‑1 (MQ=255) gcgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCgg < 1:933094/71‑1 (MQ=255) gcgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCgg < 1:3197870/71‑1 (MQ=255) gcgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTCGTGACGATACTTAACGCGCGGGTCGTCgg < 1:4412169/71‑1 (MQ=255) cgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGa < 1:4305943/71‑1 (MQ=255) cgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGa < 1:1705868/71‑1 (MQ=255) cgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGa < 1:2947960/71‑1 (MQ=255) ggCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGa > 1:3225689/1‑43 (MQ=255) ggCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGcc > 1:3325683/1‑71 (MQ=255) gCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAAt > 1:153602/1‑67 (MQ=255) cTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGcc > 1:452392/1‑69 (MQ=255) cTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGcc > 1:961029/1‑69 (MQ=255) cTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGcc > 1:2859559/1‑69 (MQ=255) tCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGcc > 1:4384031/1‑68 (MQ=255) ccgccgTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAg < 1:3281071/70‑1 (MQ=255) cgccgTTTCCCGATACACTGGCGAATCGCTGGTGAc < 1:4251742/36‑1 (MQ=255) cgccgTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCgg < 1:65595/60‑1 (MQ=255) cgccgTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGAt < 1:575348/71‑1 (MQ=255) | AAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGAT > minE/1118383‑1118520 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |