| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,136,538 | T→C | 39.7% | K129K (AAA→AAG) | yniB ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,136,538 | 0 | T | C | 39.7% | 40.6 / 75.6 | 68 | K129K (AAA→AAG) | yniB | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (17/24); new base C (13/14); total (30/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
GAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGG > minE/1136472‑1136608 | gAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTgg > 1:4333386/1‑71 (MQ=255)gAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTgg < 1:830604/71‑1 (MQ=255) aaaTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTGACGCGTTAGCCCCTCTTCCCCTTTGGCt < 1:2514627/71‑1 (MQ=255) aTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCtct < 1:1839950/71‑3 (MQ=255) aTAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCATCTTCCCCCTTGGCttt < 1:3430574/71‑1 (MQ=255) gTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCttttt < 1:1808952/70‑1 (MQ=255) tATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCttttt < 1:3788931/69‑1 (MQ=255) tGGTGCGGAACAACAATACGGGACTCAATTTGTTCCCGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTc > 1:2274411/1‑68 (MQ=255) tGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAg < 1:331186/71‑1 (MQ=255) ggTGCGGAACAACAATACGGGACTCAATTTGTTCACGGGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAg < 1:1303004/70‑1 (MQ=255) ggTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAg < 1:1880778/70‑1 (MQ=255) cGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCa > 1:2128084/1‑65 (MQ=255) ggAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCTCCCTTGGCTTTTTCCAGAATGa < 1:68959/70‑1 (MQ=255) aacaacAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAg > 1:3182386/1‑69 (MQ=255) acaacaATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTg < 1:442104/71‑1 (MQ=255) caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCtttt > 1:4681897/1‑53 (MQ=255) caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCt > 1:2045915/1‑71 (MQ=255) caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCt > 1:4212213/1‑71 (MQ=255) aaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGaa > 1:4642585/1‑59 (MQ=255) aTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc < 1:1004719/71‑1 (MQ=255) aTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc < 1:3517158/71‑1 (MQ=255) aTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTc < 1:2106881/71‑1 (MQ=255) tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc < 1:3407409/70‑1 (MQ=255) tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGaa > 1:363458/1‑57 (MQ=255) tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGaa > 1:2176401/1‑57 (MQ=255) tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGaa > 1:383360/1‑57 (MQ=255) tACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCa < 1:2036884/71‑1 (MQ=255) gggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCaa < 1:4247959/69‑1 (MQ=255) gggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATCAGTTGATCTTCaa < 1:2479995/69‑1 (MQ=255) ggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGAt < 1:4637335/62‑1 (MQ=255) aCTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCt < 1:2921203/71‑1 (MQ=255) tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGc < 1:1080943/38‑1 (MQ=255) tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGc < 1:330124/38‑1 (MQ=255) tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAg < 1:2762621/47‑1 (MQ=255) tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTg < 1:3483676/56‑1 (MQ=255) tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTc > 1:4565706/1‑71 (MQ=255) cAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTcc > 1:3145928/1‑71 (MQ=255) cAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTc > 1:1039764/1‑70 (MQ=255) aaTTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTg < 1:1954897/54‑1 (MQ=255) aaTTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCaa > 1:1238011/1‑62 (MQ=255) aaTTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTcc > 1:2720687/1‑70 (MQ=255) aaTTTGTTCACGCGTTAGCCCCTCTTCCCCCTTgg > 1:4567393/1‑35 (MQ=255) tttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAg < 1:2491188/43‑1 (MQ=255) ttGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGc > 1:3355728/1‑70 (MQ=255) ttGTTCACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGc > 1:2154554/1‑70 (MQ=255) ttCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaag < 1:360923/70‑1 (MQ=255) tctcgcgTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCagga < 1:95016/67‑1 (MQ=255) cACGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGaa > 1:1596760/1‑39 (MQ=255) cACGCGTTAGCCACTCTTCCCCTTTGGCTTTTTCCAGaa > 1:3966245/1‑39 (MQ=255) aCGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAAt < 1:3509533/39‑1 (MQ=255) aCGCGTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAAt < 1:2438034/39‑1 (MQ=255) gcgTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATg > 1:3661442/1‑38 (MQ=255) gcgTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATc > 1:2178903/1‑46 (MQ=255) gTTAGCCCCTCTTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcgc > 1:824442/1‑71 (MQ=255) cccTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCTCAAGAAGCGCGCCTGGCg < 1:2551347/71‑1 (MQ=255) cccTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCg < 1:17194/71‑1 (MQ=255) ctctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGAc > 1:9170/1‑71 (MQ=255) ctctTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCt < 1:1621011/46‑1 (MQ=255) ctctTCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGAc > 1:3963412/1‑71 (MQ=255) tctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCt < 1:1032232/36‑1 (MQ=255) tctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTc < 1:847534/38‑1 (MQ=255) tctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGAc > 1:2257440/1‑70 (MQ=255) ctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcgc > 1:566958/1‑61 (MQ=255) ctTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcgc > 1:871690/1‑61 (MQ=255) ttCCCCCTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCt > 1:2327503/1‑43 (MQ=255) tCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaag < 1:2201357/51‑1 (MQ=255) ccccTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCAt < 1:144220/70‑1 (MQ=255) ccccTTTGGCTTTTTCCAGAATGAGTTAATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCAt < 1:3725476/70‑1 (MQ=255) tttGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCggg > 1:1043158/1‑71 (MQ=255) | GAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGG > minE/1136472‑1136608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |