| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,260,264 | G→C | 6.3% | T220T (ACC→ACG) | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,260,264 | 0 | G | C | 6.3% | 225.9 / 3.8 | 79 | T220T (ACC→ACG) | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base G (45/29); new base C (2/3); total (47/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
TGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCT > minE/1260194‑1260334 | tGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAg > 1:3825630/1‑71 (MQ=255)tGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAg > 1:2003855/1‑71 (MQ=255) gCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAg < 1:972653/70‑1 (MQ=255) cAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTa < 1:2561546/71‑1 (MQ=255) aGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTAt > 1:349523/1‑71 (MQ=255) aGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTAt > 1:656751/1‑71 (MQ=255) gATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTTCTCCAGGCAATTTTTAAGGTAt > 1:3996536/1‑70 (MQ=255) ttttCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAg < 1:3159600/70‑1 (MQ=255) tttCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCg > 1:2553243/1‑71 (MQ=255) tttCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCg > 1:1779133/1‑71 (MQ=255) aGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGtt < 1:1694908/71‑1 (MQ=255) aGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGtt < 1:2379281/71‑1 (MQ=255) aGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGt < 1:3855275/70‑1 (MQ=255) aTGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCAtt > 1:1681416/1‑70 (MQ=255) tGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTcc > 1:4041160/1‑71 (MQ=255) ggAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTGAGCGTGTTCAGCGGGTTCAtcc > 1:1953086/1‑67 (MQ=255) gAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTccc < 1:3975682/70‑1 (MQ=255) aaGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCca < 1:1761453/71‑1 (MQ=255) aGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCca > 1:517849/1‑70 (MQ=255) aTTCCAGCCCTTGCTCATAGGTACTGCTCCAGGCAATTTTTAAg > 1:1856159/1‑44 (MQ=255) ttCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTa < 1:794059/46‑1 (MQ=255) ttCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGc > 1:2844393/1‑52 (MQ=255) ttCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGc > 1:1242440/1‑52 (MQ=255) ttCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGc > 1:668058/1‑52 (MQ=255) ttCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCAt < 1:3309137/60‑1 (MQ=255) ttCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTcc > 1:2033046/1‑63 (MQ=255) ttCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGca > 1:2859108/1‑71 (MQ=255) ccAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCgg > 1:196136/1‑52 (MQ=255) ccAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCa > 1:3009869/1‑57 (MQ=255) ccAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGcac > 1:869309/1‑70 (MQ=255) ccAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGcac > 1:993297/1‑70 (MQ=255) ccAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGcac > 1:1332250/1‑70 (MQ=255) ccAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGca > 1:726931/1‑69 (MQ=255) cAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTccc < 1:2669945/61‑1 (MQ=255) cAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGcacc < 1:1289954/70‑1 (MQ=255) cAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGcacc > 1:2149879/1‑70 (MQ=255) ccTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAAc > 1:3968348/1‑71 (MQ=255) cTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGtt < 1:3531775/49‑1 (MQ=255) cTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCAt > 1:2605433/1‑52 (MQ=255) ttGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGt < 1:285115/36‑1 (MQ=255) tGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTg > 1:1314854/1‑71 (MQ=255) tGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTg > 1:2656355/1‑71 (MQ=255) gCTCATTGGTACTGCTACAGGCAATTTTGAGCGTGTTCAGCGGGTTCATCCCCCACAGCACCACCACCTgg > 1:2089805/1‑71 (MQ=255) gCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCa > 1:81478/1‑39 (MQ=255) gCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTgg > 1:302596/1‑71 (MQ=255) tCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGtt < 1:686505/44‑1 (MQ=255) aTCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCCTTCCCca > 1:3453387/1‑51 (MQ=255) aTCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCca > 1:3431545/1‑51 (MQ=255) cGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCgg < 1:4459859/37‑1 (MQ=255) cGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGttt > 1:926221/1‑71 (MQ=255) tACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGtt < 1:564853/37‑1 (MQ=255) aCTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCcaca > 1:979210/1‑47 (MQ=255) tGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTccc < 1:423902/41‑1 (MQ=255) gCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAg > 1:2495951/1‑69 (MQ=255) gCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGaa > 1:4697953/1‑71 (MQ=255) gCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGaa > 1:4658312/1‑71 (MQ=255) gCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGa > 1:652890/1‑70 (MQ=255) attCCACGCAATTTTCAGCGTATTGAGTGGGTTAGCACTCCACAGCACCACGACATCGCTATGTTCCAg < 1:2550735/67‑1 (MQ=255) attCCACGCAATTTTCAGCGTATTGAGTGGGTTAGCACTCCACAGCACCACGACATCGCTATGTTCCAg < 1:1832019/67‑1 (MQ=255) ttCCACGCAATTTTCAGCGTATTGAGTGGGTTAGCACTCCACAGCACCACGACATCGCTATGTTCCAg < 1:3821885/67‑1 (MQ=255) cTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAg < 1:2248890/44‑1 (MQ=255) cTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAg < 1:1053278/44‑1 (MQ=255) cTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGcaccac > 1:2320586/1‑50 (MQ=255) cTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGa < 1:2045695/69‑1 (MQ=255) cTCCAGGCAATTTTTAAGGTATTCAGCGGGTACATTCCCCACAGCACCACAAc > 1:2576371/1‑53 (MQ=255) cTCCAGGAAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACaa > 1:4329194/1‑52 (MQ=255) tCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAAt > 1:917040/1‑70 (MQ=255) cAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATc < 1:911493/69‑1 (MQ=255) cAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAg > 1:2762794/1‑71 (MQ=255) gCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTg < 1:3645029/54‑1 (MQ=255) cAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGc > 1:3199586/1‑71 (MQ=255) aaTTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGcc < 1:1271933/71‑1 (MQ=255) tttttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGtt < 1:4597774/52‑1 (MQ=255) tttttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGtt < 1:2998249/52‑1 (MQ=255) tttttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCag > 1:2586623/1‑71 (MQ=255) tttttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCag < 1:1732167/71‑1 (MQ=255) ttttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGaa > 1:1924374/1‑59 (MQ=255) tttAAGGTATTCAGCGGGTTCATTCCCCACAGcacca > 1:1992032/1‑37 (MQ=255) tttAAGGTATTCAGCGGGTTCATTCCCCACAGcacca > 1:2004359/1‑37 (MQ=255) tttAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCg > 1:2334487/1‑64 (MQ=255) tAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag < 1:4109742/70‑1 (MQ=255) tAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCaggag < 1:91989/70‑1 (MQ=255) aGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGt < 1:2645756/70‑1 (MQ=255) ggTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTct > 1:264036/1‑71 (MQ=255) | TGCCAGATTTTTTCAGCTGATGGAAGTATTCCAGCCCTTGCTCATCGGTACTGCTCCAGGCAATTTTTAAGGTATTCAGCGGGTTCATTCCCCACAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCT > minE/1260194‑1260334 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |