| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,266,955 | T→C | 46.2% | E151G (GAA→GGA) | yecA ← | conserved metal‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,266,955 | 0 | T | C | 46.2% | 11.0 / 103.9 | 78 | E151G (GAA→GGA) | yecA | conserved metal‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (20/22); new base C (14/22); total (34/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
CCAGTCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTGACCAGT > minE/1266887‑1267021 | ccAGTCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTcc > 1:213119/1‑71 (MQ=255) aGTCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTc < 1:1833983/71‑1 (MQ=255) tCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCTACATCTTTTCTACCCGCTCGAAGTTTCCCTc < 1:1692001/69‑1 (MQ=255) gAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTa > 1:1681569/1‑71 (MQ=255) gAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCAGTa > 1:2507898/1‑71 (MQ=255) gAATGGCATCTACGCTCTCTTCAAACGCTTATGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCAGTa > 1:3577103/1‑71 (MQ=255) aaTGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTAACCGCTCGAAGTTTTCCTCAGTa < 1:624349/70‑1 (MQ=255) tGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCg < 1:2722254/71‑1 (MQ=255) tGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCAGTACCg < 1:3016698/71‑1 (MQ=255) gCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTg < 1:4270572/71‑1 (MQ=255) gCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTg > 1:1534242/1‑71 (MQ=255) tACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCa > 1:4132345/1‑58 (MQ=255) cGCTCTCTTCAAACTCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAg < 1:2815013/67‑1 (MQ=255) gCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCaa < 1:4507166/71‑1 (MQ=255) tctTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATc < 1:2783428/69‑1 (MQ=255) tctTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATc < 1:2515622/69‑1 (MQ=255) tctTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATc < 1:1166101/69‑1 (MQ=255) ctTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCa > 1:2321098/1‑60 (MQ=255) ttCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCt > 1:3672578/1‑71 (MQ=255) ttCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCACTCAGTACCGTGCAGCGCaa > 1:1624467/1‑65 (MQ=255) cAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTc < 1:4052398/70‑1 (MQ=255) cAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCAg > 1:2700578/1‑48 (MQ=255) cAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTc < 1:4332699/70‑1 (MQ=255) aaaCGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTcc < 1:960689/70‑1 (MQ=255) aaaCGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTcc < 1:3386258/70‑1 (MQ=255) aaaCGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTc < 1:2320577/45‑1 (MQ=255) aaCGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTccc > 1:1744969/1‑42 (MQ=255) aCGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCa < 1:1793680/59‑1 (MQ=255) cGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAgcg > 1:3734278/1‑71 (MQ=255) cGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCa > 1:2888662/1‑43 (MQ=255) cGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAgcg < 1:3728171/71‑1 (MQ=255) gCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAgcgc > 1:1032961/1‑71 (MQ=255) cTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCt > 1:1506488/1‑71 (MQ=255) ttCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTg > 1:3929480/1‑71 (MQ=255) cTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTg < 1:3312356/69‑1 (MQ=255) tGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGt < 1:3031944/44‑1 (MQ=255) ggCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCAGTACCGTGc < 1:2433521/45‑1 (MQ=255) ggCGACATCTTTTCTACCCGCTCGAAGTTTCCCTCAGTACCGTGc < 1:4392014/45‑1 (MQ=255) gCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGtt < 1:4396188/69‑1 (MQ=255) gCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGt < 1:994662/68‑1 (MQ=255) cGACATCTTTTCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGtttt > 1:4631213/1‑70 (MQ=255) gACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTaa < 1:2665451/71‑1 (MQ=255) cATCTTTTCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAAt > 1:4626276/1‑48 (MQ=255) aTCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCaa > 1:348555/1‑46 (MQ=255) aTCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGa < 1:2456398/71‑1 (MQ=255) tCTTTTCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCa < 1:1323083/44‑1 (MQ=255) cTTTTCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAgcgc < 1:2184033/42‑1 (MQ=255) ttttCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTaa > 1:4224901/1‑65 (MQ=255) ttttCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGa < 1:1529109/68‑1 (MQ=255) ttttCTACCCGCTCGAAGTTTCCCTCAGAACCGTGc > 1:252705/1‑36 (MQ=38) tttCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAgc < 1:573801/53‑1 (MQ=255) ttCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCgg > 1:3919484/1‑71 (MQ=255) ttCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCgg > 1:3013056/1‑71 (MQ=255) ttCTACCCGCTCGAAGTTTCCCTCAGTTCCGTGCAGCGCAATCGCCTCCAGCGCTGGtttt < 1:2103441/61‑1 (MQ=255) ttCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGaa < 1:2267602/67‑1 (MQ=255) ttCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGaa < 1:3355284/67‑1 (MQ=255) ttCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGaa < 1:2857452/67‑1 (MQ=255) ttCTACCCGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCgg > 1:2682462/1‑71 (MQ=255) cccGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAAc < 1:2263027/71‑1 (MQ=255) ccGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACg < 1:1198140/71‑1 (MQ=255) ccGCTCGAAGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACg < 1:124726/71‑1 (MQ=255) gCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGtt > 1:1356164/1‑71 (MQ=255) gCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGtt < 1:2784948/71‑1 (MQ=255) cTCGAAGTTTCCCTCACTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTg < 1:4292016/71‑1 (MQ=255) tCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAg > 1:1983153/1‑41 (MQ=255) tCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAg > 1:3085914/1‑41 (MQ=255) cGAAGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGaa > 1:663661/1‑56 (MQ=255) gAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTTGTTTTAACGAATCGGGCAACGTTGAcc < 1:1409400/71‑1 (MQ=255) gAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTGAc > 1:1945148/1‑70 (MQ=255) gAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTGAc > 1:3565639/1‑70 (MQ=255) aaGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTGAc < 1:3686034/69‑1 (MQ=255) aaGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTGAc < 1:425726/69‑1 (MQ=255) aGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGaa > 1:4505891/1‑53 (MQ=255) aGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAAt > 1:3549108/1‑54 (MQ=255) aGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAAt > 1:754144/1‑54 (MQ=255) aGTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTGACCAg < 1:4318554/71‑1 (MQ=255) gTTTTCCTCAGTACCGTGCAGAGCAATCGCCTCCAGCGCTGGTTTTAACGAAt > 1:175817/1‑53 (MQ=255) gTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTGACCAGt > 1:1477632/1‑71 (MQ=255) gTTTCCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTGACCAGt < 1:3565911/71‑1 (MQ=255) ttttCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAGTCGGGCAACGTTGa < 1:4125537/65‑1 (MQ=255) ttttCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAAc < 1:1261730/60‑1 (MQ=255) ttCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGtt > 1:1978829/1‑41 (MQ=255) | CCAGTCGAATGGCATCTACGCTCTCTTCAAACGCTTCTGGCGACATCTTTTCTACCCGCTCGAAGTTTTCCTCAGTACCGTGCAGCGCAATCGCCTCCAGCGCTGGTTTTAACGAATCGGGCAACGTTGACCAGT > minE/1266887‑1267021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |