| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 194,465 | C→T | 5.2% | G124G (GGC→GGT) | nlpE → | lipoprotein involved with copper homeostasis and adhesion |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 194,465 | 0 | C | T | 5.2% | 302.0 / 4.2 | 96 | G124G (GGC→GGT) | nlpE | lipoprotein involved with copper homeostasis and adhesion |
| Reads supporting (aligned to +/- strand): ref base C (40/51); new base T (2/3); total (42/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GACAGCAAAGGTGAAAAGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGA > minE/194397‑194535 | gACAGCAAAGGTGAAAAGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCaa > 1:4456785/1‑71 (MQ=255) aGCAAAGGTGAAAAGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATcc > 1:2782839/1‑71 (MQ=255) aGGTGAAAAGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGAtt > 1:462101/1‑70 (MQ=255) aGGTGAAAAGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTg < 1:1244676/71‑1 (MQ=255) aGGTGAAAAGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTg < 1:3297494/71‑1 (MQ=255) aGGTGAAAAGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTg < 1:4545705/71‑1 (MQ=255) aGGTGAAAAGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTg < 1:4288525/71‑1 (MQ=255) gAAAAGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGa > 1:3897572/1‑64 (MQ=255) gAAAAGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATc > 1:3793905/1‑71 (MQ=255) aaaaGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCg > 1:3499623/1‑71 (MQ=255) aaaaGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCg < 1:4175524/71‑1 (MQ=255) aaaGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCg > 1:932418/1‑70 (MQ=255) aaaGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCg > 1:3678459/1‑70 (MQ=255) aaaGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCg < 1:3576062/70‑1 (MQ=255) aGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCCATCGTGAAGGCAATCCGATTGAATCGCAg > 1:4284955/1‑71 (MQ=255) tCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGTAATCCg < 1:604811/57‑1 (MQ=255) tCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGa < 1:1777231/62‑1 (MQ=255) tCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGtt > 1:3228437/1‑71 (MQ=255) tCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGtt < 1:4648619/71‑1 (MQ=255) aggattatCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTc < 1:2244822/68‑1 (MQ=255) atatTATCGGGCGAAATGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTc < 1:4695594/70‑1 (MQ=255) atatTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGTCAATCCGATTGAATCGCAGTTCa < 1:3642481/71‑1 (MQ=255) atatTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAAt > 1:2527190/1‑62 (MQ=255) atatTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTc < 1:1011317/70‑1 (MQ=255) atatTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCa < 1:3581380/71‑1 (MQ=255) atatTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCa < 1:2975481/71‑1 (MQ=255) tattatCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCa < 1:2719977/70‑1 (MQ=255) attatCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGa > 1:221517/1‑54 (MQ=255) ttatCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAAc > 1:3368762/1‑70 (MQ=255) ttatCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAAc > 1:1577817/1‑70 (MQ=255) atCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGAt > 1:2404855/1‑52 (MQ=255) atCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACta > 1:4584889/1‑70 (MQ=255) atCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACta > 1:1469736/1‑70 (MQ=255) atCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGCCAATCCGATTGAATCGCAGTTCAACta < 1:704614/70‑1 (MQ=255) tCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCa < 1:4573381/65‑1 (MQ=255) tCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCa < 1:1278495/65‑1 (MQ=255) tCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACtat < 1:4179999/70‑1 (MQ=255) gggCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATAc < 1:738025/70‑1 (MQ=255) gAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTgg < 1:2908823/71‑1 (MQ=255) aGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCg > 1:4021914/1‑49 (MQ=255) aGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAg < 1:4565119/71‑1 (MQ=255) gCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCa > 1:1654710/1‑49 (MQ=255) gCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCa > 1:649391/1‑49 (MQ=255) gCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAg > 1:66758/1‑69 (MQ=255) tGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTc < 1:398715/49‑1 (MQ=255) tGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGca > 1:451964/1‑70 (MQ=255) gcgcTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCa > 1:1111353/1‑44 (MQ=255) gcgcTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGcac > 1:3954650/1‑70 (MQ=255) cgcTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACaa > 1:669168/1‑71 (MQ=255) cgcTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACaa < 1:2364849/71‑1 (MQ=255) gcTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAAt < 1:1008027/71‑1 (MQ=255) cTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCg < 1:3426459/39‑1 (MQ=255) cTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGaa < 1:478022/60‑1 (MQ=255) tGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAAc < 1:3953316/47‑1 (MQ=255) tGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATAc < 1:3525800/52‑1 (MQ=255) ggAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATc > 1:1207774/1‑69 (MQ=255) gagaTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAg < 1:3656789/71‑1 (MQ=255) gaTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAg > 1:2308885/1‑37 (MQ=255) gaTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAg > 1:39843/1‑37 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCa < 1:99361/40‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCa < 1:2322318/40‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCa < 1:2843740/40‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGca < 1:106694/60‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGca < 1:176665/60‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGttt < 1:3965547/71‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGttt < 1:2139750/71‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGtt > 1:1655820/1‑70 (MQ=255) tGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTc < 1:1063174/38‑1 (MQ=255) gCTCGATCGTGAAGGCAATCCGATTGAATCGCAGt > 1:2993355/1‑35 (MQ=255) gCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCa < 1:3553370/38‑1 (MQ=255) gCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGa > 1:3694785/1‑51 (MQ=255) gCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGa > 1:592710/1‑51 (MQ=255) gCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTAc > 1:1505101/1‑71 (MQ=255) cTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACt < 1:821580/40‑1 (MQ=255) cTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTAcc < 1:3808556/71‑1 (MQ=255) cTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTAcc > 1:3386455/1‑71 (MQ=255) cGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAg < 1:16879/63‑1 (MQ=255) gATCGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGaacc > 1:2304392/1‑48 (MQ=38) gATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCt < 1:2899788/69‑1 (MQ=255) gATCGTCAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGaacc > 1:7713/1‑48 (MQ=255) ttCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTa < 1:1510806/68‑1 (MQ=255) aTCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTAt < 1:3578378/70‑1 (MQ=255) tCGTGAAGGCAATCCGATTGAATCGCAGTTCAACtata > 1:4332511/1‑38 (MQ=255) tCGTGAAGGCAATCCGATTGAATCGCAGTTCAACtata > 1:1148640/1‑38 (MQ=255) tCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGa > 1:3613669/1‑71 (MQ=255) cGTGAAGGCACTCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACaa > 1:2655993/1‑54 (MQ=255) gTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTgg > 1:2137827/1‑42 (MQ=255) gTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTgg > 1:4400753/1‑42 (MQ=255) gTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGcac < 1:2963225/51‑1 (MQ=255) tGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGc < 1:1632245/45‑1 (MQ=255) tGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGttt < 1:3956167/60‑1 (MQ=255) gAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCt < 1:2622143/38‑1 (MQ=38) aaGGTAATCCGATTGAATCGCAGTTCAACTACACGCt < 1:4123140/37‑1 (MQ=255) aaGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGcc < 1:4034735/70‑1 (MQ=255) aaGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGcc < 1:615370/70‑1 (MQ=255) gCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATg < 1:2746143/71‑1 (MQ=255) cAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGa < 1:1973698/71‑1 (MQ=255) | GACAGCAAAGGTGAAAAGTCATATTATCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGA > minE/194397‑194535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |