BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,456,471	A→G	6.8%	T293T (ACT→ACC) ‡	yfaQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,456,471	0	A	G	6.8%	314.1 / 11.9	117	T293T (ACT→ACC) ‡	yfaQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (57/52); new base G (5/3); total (62/55)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01

Only 100 of 119 total aligned reads displayed.

TTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGT  >  minE/1456402‑1456539

tttCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAg                                                                     >  1:1116040/1‑71 (MQ=255)

tttCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAg                                                                     >  1:3221115/1‑71 (MQ=255)

   cAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTa                                                                   <  1:3231928/70‑1 (MQ=255)

   cAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTa                                                                   <  1:4932/70‑1 (MQ=255)

    aGTTTGCCCTTTACCGTCAGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCaaa                                                                 >  1:2315169/1‑71 (MQ=255)

    aGTTTGCCCTTTACCGTCAGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCaaa                                                                 >  1:1680379/1‑71 (MQ=255)

    aGTTTGCCCTTTACCGTCAGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCaaa                                                                 >  1:1674873/1‑71 (MQ=255)

      tttGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACg                                                               <  1:13248/71‑1 (MQ=255)

       ttGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                              >  1:3505648/1‑71 (MQ=255)

        tGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                              >  1:2697533/1‑70 (MQ=255)

        tGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                              >  1:2447968/1‑70 (MQ=255)

        tGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                              <  1:2134998/70‑1 (MQ=255)

        tGCCCTTTACCGTCAGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCAAACGt                                                              >  1:2971241/1‑70 (MQ=255)

        tGCCCTTTACCGTCAGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCAAACGt                                                              >  1:1692362/1‑70 (MQ=255)

           ccTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAg                                                                     <  1:3021676/60‑1 (MQ=255)

            cTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCacac                                                         >  1:3888163/1‑71 (MQ=255)

            cTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCacac                                                         <  1:1152635/71‑1 (MQ=255)

             ttttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTa                                                                   >  1:391643/1‑60 (MQ=255)

             ttttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCacac                                                         >  1:3697555/1‑70 (MQ=255)

             ttttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCacac                                                         >  1:1601895/1‑70 (MQ=255)

              tttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGa                                                       >  1:3264045/1‑71 (MQ=255)

               ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaaa                                                                 >  1:321178/1‑60 (MQ=255)

               ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                              <  1:3959193/63‑1 (MQ=255)

               ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTca                                                            >  1:1224652/1‑65 (MQ=255)

               ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCacac                                                         >  1:18325/1‑68 (MQ=255)

               ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGa                                                       <  1:3672799/70‑1 (MQ=255)

               ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGa                                                       >  1:4334142/1‑70 (MQ=255)

               ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGa                                                       >  1:137050/1‑70 (MQ=255)

                tCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGa                                                       >  1:3259148/1‑69 (MQ=255)

                tCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                      <  1:1271663/70‑1 (MQ=255)

                 cGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAg                                                    >  1:4270240/1‑71 (MQ=255)

                  gCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAgc                                                   <  1:3394554/71‑1 (MQ=255)

                      aGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaaa                                                                 >  1:227404/1‑53 (MQ=255)

                      aGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaaa                                                                 >  1:1270617/1‑53 (MQ=255)

                      aGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGcc                                                <  1:3348072/70‑1 (MQ=255)

                      aGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCAAACGTCACACGATAGCGccc                                               <  1:1940605/71‑1 (MQ=255)

                      aGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCAAACGTCACACGATAGCGccc                                               <  1:1834628/71‑1 (MQ=255)

                       gTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaa                                                                  <  1:2869499/51‑1 (MQ=255)

                       gTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                              <  1:2397824/55‑1 (MQ=255)

                        tAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAAc                                                                <  1:2532098/52‑1 (MQ=255)

                         aaGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATa                                                     >  1:2395052/1‑62 (MQ=255)

                          aGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTa                                                                      >  1:3256163/1‑44 (MQ=255)

                          aGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaaa                                                                 >  1:1235974/1‑49 (MQ=255)

                          aGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCAtt                                            <  1:3998669/70‑1 (MQ=255)

                          aGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCAtt                                            <  1:677331/70‑1 (MQ=255)

                            gtcgtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAg                                         >  1:4048335/1‑71 (MQ=255)

                            gtcgtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAg                                         >  1:1385361/1‑71 (MQ=255)

                            gtcgtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAg                                         <  1:2879858/71‑1 (MQ=255)

                             tcgtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAAc                                                                <  1:1136731/47‑1 (MQ=255)

                              cgtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTa                                       >  1:1801367/1‑71 (MQ=255)

                               gtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTAc                                      <  1:569516/71‑1 (MQ=255)

                                tctcACCGTGTCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCa                                              <  1:750713/62‑1 (MQ=255)

                                tctcACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTa                                           <  1:4150697/65‑1 (MQ=255)

                                tctcACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTAc                                      <  1:4438271/70‑1 (MQ=255)

                                  tcACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAAc                                                                <  1:1480045/42‑1 (MQ=255)

                                  tcACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTca                                                            <  1:698543/46‑1 (MQ=255)

                                  tcACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAg                                         <  1:135956/65‑1 (MQ=255)

                                  tcACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGcc                                   >  1:964757/1‑71 (MQ=255)

                                   cACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCAtt                                            <  1:1066110/61‑1 (MQ=255)

                                   cACCGTGCCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                      >  1:4695925/1‑51 (MQ=255)

                                    aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTca                                                            >  1:614383/1‑44 (MQ=255)

                                    aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                      >  1:483005/1‑50 (MQ=255)

                                    aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGt                                        <  1:925089/64‑1 (MQ=255)

                                    aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCgg                                 >  1:2673482/1‑71 (MQ=255)

                                    aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGT‑‑CACGATAGCGCCCATTAAGTACGCCGGGa                               >  1:1786018/1‑71 (MQ=255)

                                     ccGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCgg                                 >  1:3444388/1‑70 (MQ=255)

                                       gTGGCTGACAAACGTAATGTGGTTACCATTAGTAAAc                                                                >  1:21186/1‑37 (MQ=255)

                                        tGGCTGACAAACGTAATGTGGTTACCATTAGTaaa                                                                 >  1:2758341/1‑35 (MQ=255)

                                          gCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                      >  1:4643617/1‑44 (MQ=255)

                                          gCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGAttt                            <  1:2443786/70‑1 (MQ=255)

                                          gCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTa                           >  1:963760/1‑71 (MQ=255)

                                           cTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCgg                                 <  1:3922471/64‑1 (MQ=255)

                                            tGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGAttt                            <  1:3843245/68‑1 (MQ=255)

                                              aCAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACt                         <  1:250390/69‑1 (MQ=255)

                                              aCAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACt                         <  1:2684853/69‑1 (MQ=255)

                                                aaaCGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                      >  1:2058863/1‑38 (MQ=255)

                                                 aaCGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGa                               >  1:952922/1‑60 (MQ=255)

                                                   cGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAg                                         <  1:3980611/48‑1 (MQ=255)

                                                   cGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAg                                         <  1:1326156/48‑1 (MQ=255)

                                                    gTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTa                           >  1:3165504/1‑61 (MQ=255)

                                                    gTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTa                           >  1:3481204/1‑61 (MQ=255)

                                                    gTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCcgtcgt                 <  1:2058921/71‑1 (MQ=255)

                                                     tAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTa                <  1:259839/71‑1 (MQ=255)

                                                      aaTGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCgg                                 >  1:1588562/1‑53 (MQ=255)

                                                      aaTGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTAc               >  1:1947000/1‑71 (MQ=255)

                                                       aTGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTAct              >  1:3837791/1‑71 (MQ=255)

                                                          tgGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTAGGCCGGGATTTACTGCCGTCGTACTCtt           >  1:4260870/1‑71 (MQ=255)

                                                          tgGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGAtt                             <  1:1457102/53‑1 (MQ=255)

                                                          tgGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTa                           >  1:101241/1‑55 (MQ=255)

                                                          tgGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTa                           >  1:2309393/1‑55 (MQ=255)

                                                          tgGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCtt           <  1:4343233/71‑1 (MQ=255)

                                                            gTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCttt          <  1:4514980/70‑1 (MQ=255)

                                                            gTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCttt          <  1:2013161/70‑1 (MQ=255)

                                                            gTTACCATTAATAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCttt          <  1:3346716/70‑1 (MQ=255)

                                                             ttACCATTGGCAAACGTCACACGATAGCGCCCATTGAGCACGCCTGGTTTGAATGCcg                     <  1:961742/58‑1 (MQ=255)

                                                              tACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCttta         >  1:4301983/1‑68 (MQ=255)

                                                                ccATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCg      <  1:3023854/70‑1 (MQ=255)

                                                                  aTTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCcgtcg                  <  1:3063899/56‑1 (MQ=255)

                                                                   ttAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGAtt                             <  1:241550/44‑1 (MQ=255)

                                                                     aGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTa                >  1:2846963/1‑55 (MQ=255)

TTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGT  >  minE/1456402‑1456539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`