BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,456,473	T→C	6.8%	T293A (ACT→GCT) ‡	yfaQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,456,473	0	T	C	6.8%	320.0 / 11.3	118	T293A (ACT→GCT) ‡	yfaQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (57/53); new base C (5/3); total (62/56)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATT  >  minE/1456405‑1456543

cAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTa                                                                       <  1:3231928/70‑1 (MQ=255)

cAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTa                                                                       <  1:4932/70‑1 (MQ=255)

 aGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaaa                                                                     <  1:197072/71‑1 (MQ=255)

 aGTTTGCCCTTTACCGTCAGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCaaa                                                                     >  1:2315169/1‑71 (MQ=255)

 aGTTTGCCCTTTACCGTCAGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCaaa                                                                     >  1:1680379/1‑71 (MQ=255)

 aGTTTGCCCTTTACCGTCAGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCaaa                                                                     >  1:1674873/1‑71 (MQ=255)

   tttGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACg                                                                   <  1:13248/71‑1 (MQ=255)

    ttGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                                  >  1:3505648/1‑71 (MQ=255)

     tGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                                  <  1:2134998/70‑1 (MQ=255)

     tGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                                  >  1:2447968/1‑70 (MQ=255)

     tGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                                  >  1:2697533/1‑70 (MQ=255)

     tGCCCTTTACCGTCAGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCAAACGt                                                                  >  1:1692362/1‑70 (MQ=255)

     tGCCCTTTACCGTCAGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCAAACGt                                                                  >  1:2971241/1‑70 (MQ=255)

         cTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCacac                                                             <  1:1152635/71‑1 (MQ=255)

         cTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCacac                                                             >  1:3888163/1‑71 (MQ=255)

          ttttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTa                                                                       >  1:391643/1‑60 (MQ=255)

          ttttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCacac                                                             >  1:1601895/1‑70 (MQ=255)

          ttttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCacac                                                             >  1:3697555/1‑70 (MQ=255)

           tttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGa                                                           >  1:3264045/1‑71 (MQ=255)

            ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaaa                                                                     >  1:321178/1‑60 (MQ=255)

            ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                                  <  1:3959193/63‑1 (MQ=255)

            ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTca                                                                >  1:1224652/1‑65 (MQ=255)

            ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCacac                                                             >  1:18325/1‑68 (MQ=255)

            ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACg                                                            <  1:3956888/69‑1 (MQ=255)

            ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGa                                                           >  1:137050/1‑70 (MQ=255)

            ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGa                                                           >  1:4334142/1‑70 (MQ=255)

            ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGa                                                           <  1:3672799/70‑1 (MQ=255)

            ttCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGa                                                           <  1:375351/70‑1 (MQ=255)

             tCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                          <  1:1271663/70‑1 (MQ=255)

              cGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAg                                                        >  1:4270240/1‑71 (MQ=255)

               gCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAgc                                                       <  1:3394554/71‑1 (MQ=255)

                   aGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaaa                                                                     >  1:1270617/1‑53 (MQ=255)

                   aGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaaa                                                                     >  1:4490122/1‑53 (MQ=255)

                   aGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGcc                                                    <  1:3348072/70‑1 (MQ=255)

                   aGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCAAACGTCACACGATAGCGccc                                                   <  1:1940605/71‑1 (MQ=255)

                   aGTAACGTTGTCTCACCGTGGCTGACAAATGTCATGTGGTTACCATTGGCAAACGTCACACGATAGCGccc                                                   <  1:1834628/71‑1 (MQ=255)

                    gTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTaa                                                                      <  1:2869499/51‑1 (MQ=255)

                    gTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGt                                                                  <  1:2397824/55‑1 (MQ=255)

                     tAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAAc                                                                    <  1:2532098/52‑1 (MQ=255)

                      aaGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATa                                                         >  1:2395052/1‑62 (MQ=255)

                       aGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCAtt                                                <  1:3998669/70‑1 (MQ=255)

                       aGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCAtt                                                <  1:1787811/70‑1 (MQ=255)

                       aGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCAtt                                                <  1:677331/70‑1 (MQ=255)

                         gtcgtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAAc                                                                    >  1:285598/1‑48 (MQ=255)

                         gtcgtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAg                                             >  1:4048335/1‑71 (MQ=255)

                         gtcgtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAg                                             <  1:2879858/71‑1 (MQ=255)

                         gtcgtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAg                                             >  1:1385361/1‑71 (MQ=255)

                          tcgtcTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAAc                                                                    <  1:1136731/47‑1 (MQ=255)

                             tctcACCGTGTCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCa                                                  <  1:750713/62‑1 (MQ=255)

                             tctcACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTa                                               <  1:4150697/65‑1 (MQ=255)

                             tctcACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTAc                                          <  1:4438271/70‑1 (MQ=255)

                               tcACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAAc                                                                    <  1:1480045/42‑1 (MQ=255)

                               tcACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTca                                                                <  1:698543/46‑1 (MQ=255)

                               tcACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGcc                                       >  1:964757/1‑71 (MQ=255)

                                cACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCAtt                                                <  1:1066110/61‑1 (MQ=255)

                                cACCGTGCCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                          >  1:4695925/1‑51 (MQ=255)

                                 aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTca                                                                >  1:614383/1‑44 (MQ=255)

                                 aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                          >  1:483005/1‑50 (MQ=255)

                                 aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                          >  1:153586/1‑50 (MQ=255)

                                 aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGt                                            <  1:925089/64‑1 (MQ=255)

                                 aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCgg                                     >  1:2673482/1‑71 (MQ=255)

                                 aCCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGT‑‑CACGATAGCGCCCATTAAGTACGCCGGGa                                   >  1:1786018/1‑71 (MQ=255)

                                  ccGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCgg                                     >  1:3444388/1‑70 (MQ=255)

                                    gTGGCTGACAAACGTAATGTGGTTACCATTAGTAAAc                                                                    >  1:21186/1‑37 (MQ=255)

                                     tGGCTGACAAACGTAATGTGGTTACCATTAGTaaa                                                                     >  1:2758341/1‑35 (MQ=255)

                                       gCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                          >  1:4643617/1‑44 (MQ=255)

                                       gCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGAttt                                <  1:2443786/70‑1 (MQ=255)

                                       gCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTa                               >  1:963760/1‑71 (MQ=255)

                                        cTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCgg                                     <  1:3922471/64‑1 (MQ=255)

                                         tGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGTGATTTa                               >  1:1232356/1‑69 (MQ=255)

                                           aCAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACt                             <  1:2684853/69‑1 (MQ=255)

                                           aCAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACt                             <  1:250390/69‑1 (MQ=255)

                                             aaaCGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                          >  1:2058863/1‑38 (MQ=255)

                                              aaCGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGa                                   >  1:952922/1‑60 (MQ=255)

                                                cGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAg                                             <  1:1326156/48‑1 (MQ=255)

                                                 gTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTa                               >  1:557736/1‑61 (MQ=255)

                                                 gTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTa                               >  1:3165504/1‑61 (MQ=255)

                                                 gTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCcgtcgt                     <  1:2058921/71‑1 (MQ=255)

                                                  tAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTa                    <  1:259839/71‑1 (MQ=255)

                                                   aaTGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTAc                   >  1:4201247/1‑71 (MQ=255)

                                                   aaTGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTAc                   >  1:1947000/1‑71 (MQ=255)

                                                    aTGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTAct                  >  1:3837791/1‑71 (MQ=255)

                                                     tgtgGTTACCATTAGTAAACGTCACACGATAGCGCCCAtt                                                <  1:2148208/40‑1 (MQ=255)

                                                       tgGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGAtt                                 <  1:1457102/53‑1 (MQ=255)

                                                       tgGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGAt                                  >  1:3238012/1‑52 (MQ=255)

                                                       tgGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTa                               >  1:101241/1‑55 (MQ=255)

                                                       tgGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTa                               >  1:2309393/1‑55 (MQ=255)

                                                       tgGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCtt               <  1:4343233/71‑1 (MQ=255)

                                                       tgGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCtt               <  1:1346865/71‑1 (MQ=255)

                                                         gTTACCATTAATAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCttt              <  1:3346716/70‑1 (MQ=255)

                                                          ttACCATTGGCAAACGTCACACGATAGCGCCCATTGAGCACGCCTGGTTTGAATGCcg                         <  1:961742/58‑1 (MQ=255)

                                                           tACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCttta             >  1:4301983/1‑68 (MQ=255)

                                                            aCCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCg          >  1:3413590/1‑71 (MQ=255)

                                                             ccATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCg          <  1:3023854/70‑1 (MQ=255)

                                                               aTTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCcgtcg                      <  1:3063899/56‑1 (MQ=255)

                                                                ttAGTAAACGTCCCACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCtgt      >  1:2414762/1‑71 (MQ=255)

                                                                   gTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGa    <  1:680402/70‑1 (MQ=255)

                                                                   gTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGa    <  1:2799625/70‑1 (MQ=255)

                                                                   gTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGAt   >  1:4075220/1‑71 (MQ=255)

                                                                    tAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGAtt  <  1:2587895/71‑1 (MQ=255)

CAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATT  >  minE/1456405‑1456543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`