| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,563,260 | A→G | 5.1% | E1089E (GAA→GAG) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,563,260 | 0 | A | G | 5.1% | 322.2 / 7.0 | 118 | E1089E (GAA→GAG) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base A (49/63); new base G (3/3); total (52/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
AAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAA > minE/1563192‑1563330 | aaaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGaa < 1:528121/69‑1 (MQ=255)aaaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGc < 1:1329106/71‑1 (MQ=255)aaaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGc > 1:2614347/1‑71 (MQ=255) aaaCACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGc < 1:362184/70‑1 (MQ=255) aTTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaa < 1:4507434/71‑1 (MQ=255) aTTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaa < 1:2969082/70‑1 (MQ=255) tttAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGcc < 1:905197/64‑1 (MQ=255) tAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCt > 1:2351586/1‑64 (MQ=255) tAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaaaa > 1:1469804/1‑70 (MQ=255) tAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaaaa > 1:2520576/1‑70 (MQ=255) aaGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaaa < 1:1569802/68‑1 (MQ=255) aaGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaat < 1:1895909/70‑1 (MQ=255) aaGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaat < 1:3738237/70‑1 (MQ=255) aGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataa < 1:3120305/71‑1 (MQ=255) aGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataa < 1:1016261/71‑1 (MQ=255) gTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataa > 1:2301890/1‑70 (MQ=255) cAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATAc > 1:2278491/1‑71 (MQ=255) cAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATAc > 1:2011654/1‑71 (MQ=255) aGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATAc > 1:1424044/1‑70 (MQ=255) aGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACg > 1:4658243/1‑71 (MQ=255) aGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACg > 1:1566820/1‑71 (MQ=255) tcacacCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaata < 1:3268673/62‑1 (MQ=255) tcacacCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaata < 1:3274225/62‑1 (MQ=255) tACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGc > 1:960767/1‑70 (MQ=255) acacCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCg > 1:2308176/1‑70 (MQ=255) acacCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCg > 1:292016/1‑70 (MQ=255) cacCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataat < 1:3915617/63‑1 (MQ=255) cacCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataat < 1:3098292/63‑1 (MQ=255) cacCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGa < 1:1417010/70‑1 (MQ=255) acCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGaa > 1:4285374/1‑70 (MQ=255) ccAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGaa > 1:4331337/1‑46 (MQ=255) ccAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAAc > 1:764116/1‑70 (MQ=255) ccAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACg < 1:1009963/71‑1 (MQ=255) aaaGATGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCg > 1:1875941/2‑66 (MQ=255) gTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaaaa < 1:1443936/53‑1 (MQ=255) ttGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGaa > 1:566026/1‑64 (MQ=255) ttGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTa < 1:3983995/71‑1 (MQ=255) gcgcATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACgg > 1:1458734/1‑58 (MQ=255) gcgcATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTAc > 1:3595551/1‑70 (MQ=255) gcgcATATTGCACCTCAGTATCGCCACCTTGATATAGAAGCCCTGAAAAATAATACGGCGAACGATCTAc > 1:2309807/1‑70 (MQ=255) gcATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataa < 1:3341420/51‑1 (MQ=255) cATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACaa > 1:2438373/1‑69 (MQ=255) atatTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataat > 1:3904239/1‑50 (MQ=255) atatTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACt > 1:1803222/1‑70 (MQ=255) tatTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGaaaaa > 1:1694276/1‑45 (MQ=255) tatTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAataata < 1:251593/50‑1 (MQ=255) tatTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACt < 1:940166/69‑1 (MQ=255) atTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGAt > 1:1067788/1‑71 (MQ=255) ttGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAataata > 1:2159575/1‑48 (MQ=255) ttGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATg > 1:3493686/1‑71 (MQ=255) tGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaat < 1:3721352/43‑1 (MQ=255) tGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATg < 1:198602/70‑1 (MQ=255) tGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATg > 1:2038211/1‑70 (MQ=255) gCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGa > 1:8738/1‑67 (MQ=255) ccTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAataata < 1:3874205/43‑1 (MQ=255) cTCAGTATCGCCACCTTGATATCGAGGCCCTGAAGAATAATACGGCGAATGATCt > 1:3599040/1‑55 (MQ=255) cTCAGTATCGCCACCTTGATATCGAGGCCCTGAAGAATAATACGGCGAATGATCt > 1:1007184/1‑55 (MQ=255) cTCAGTATCGCCACCTTGATATCGAGGCCCTGAAGAATAATACGGCGAATGATCt > 1:3782046/1‑55 (MQ=255) cTCAGTATCGCCACCTTGATATCGAAGCCCTGaaaaa < 1:2482688/37‑1 (MQ=255) cTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCg < 1:2715308/47‑1 (MQ=255) cTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCg < 1:4020078/47‑1 (MQ=255) cTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACg < 1:5038/51‑1 (MQ=255) tCAGTATCGCCACCTTGATATCGAAGCCCTGAAAaataa > 1:2756484/1‑39 (MQ=255) tCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACg > 1:2547287/1‑43 (MQ=255) tCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGAt > 1:4582072/1‑52 (MQ=255) cAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGAt > 1:2996509/1‑51 (MQ=255) tATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATtct < 1:1113211/71‑1 (MQ=255) aTCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATtct > 1:4282495/1‑70 (MQ=255) tCGCCACCTTGATATCGAGGCCCTGAAGAATAATACGGCGAATGATCTACAACTGATGCAGGAGATtctc < 1:4146222/70‑1 (MQ=255) tCGCCACCTTGATATCGAGGCCCTGAAGAATAATACGGCGAATGATCTACAACTGATGCAGGAGATtctc < 1:2217315/70‑1 (MQ=255) tCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATtctc < 1:534061/70‑1 (MQ=255) tCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCa > 1:1159447/1‑71 (MQ=255) tCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCa < 1:3017580/71‑1 (MQ=255) tCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCa > 1:2638426/1‑71 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt > 1:3180094/1‑70 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt > 1:388739/1‑70 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt < 1:3904116/70‑1 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt > 1:2936955/1‑70 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATg < 1:4544322/71‑1 (MQ=255) ccACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATg > 1:1110677/1‑70 (MQ=255) ccACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGa < 1:1273185/71‑1 (MQ=255) cACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGAc > 1:337009/1‑71 (MQ=255) aCCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGAt > 1:3129396/1‑41 (MQ=255) aCCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACt < 1:4297112/71‑1 (MQ=255) ccTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTAc < 1:1503776/44‑1 (MQ=255) ccTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTAc < 1:118119/44‑1 (MQ=255) cTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGga < 1:319476/56‑1 (MQ=255) cTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACttt < 1:2033620/71‑1 (MQ=255) cTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACttt < 1:1248196/71‑1 (MQ=255) cTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACttt < 1:1054867/71‑1 (MQ=255) ttGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt < 1:1206723/64‑1 (MQ=255) gATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTc < 1:2403/69‑1 (MQ=255) atatCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTGTCATGACTTTcc < 1:1846891/69‑1 (MQ=255) tatCGAAGCCCTGAAAAATAATACGGCGATCGATCTACAACTGATGCAGga > 1:4438435/1‑51 (MQ=255) tCGAAGCCCTGAAAAATAATACGGCGAACGATCTACa < 1:1166197/37‑1 (MQ=255) tCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATg < 1:3584050/44‑1 (MQ=255) tCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATg < 1:1703425/44‑1 (MQ=255) gAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATg > 1:4370306/1‑70 (MQ=255) gAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGa < 1:1239637/71‑1 (MQ=255) gAAGCCCTGAAAAATAATAC‑‑CGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGaaa > 1:3373841/1‑71 (MQ=255) | AAAACACATTTAAGTCAGTTACACCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAA > minE/1563192‑1563330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |