| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 194,492 | T→C | 5.6% | Y133Y (TAT→TAC) | nlpE → | lipoprotein involved with copper homeostasis and adhesion |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 194,492 | 0 | T | C | 5.6% | 192.6 / 2.9 | 71 | Y133Y (TAT→TAC) | nlpE | lipoprotein involved with copper homeostasis and adhesion |
| Reads supporting (aligned to +/- strand): ref base T (28/39); new base C (2/2); total (30/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 | |||||||||||
TCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATG > minE/194423‑194561 | tCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACtat < 1:4179999/70‑1 (MQ=255) gggCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATAc < 1:738025/70‑1 (MQ=255) gAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTgg < 1:2908823/71‑1 (MQ=255) aGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAg < 1:4565119/71‑1 (MQ=255) gCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAg > 1:66758/1‑69 (MQ=255) tGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGca > 1:451964/1‑70 (MQ=255) gcgcTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGcac > 1:3954650/1‑70 (MQ=255) cgcTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACaa < 1:2364849/71‑1 (MQ=255) cgcTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACaa > 1:669168/1‑71 (MQ=255) gcTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAAt < 1:1008027/71‑1 (MQ=255) cTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGaa < 1:478022/60‑1 (MQ=255) tGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATAc < 1:3525800/52‑1 (MQ=255) ggAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATc > 1:1207774/1‑69 (MQ=255) gagaTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAg < 1:3656789/71‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGca < 1:176665/60‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGca < 1:106694/60‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGttt < 1:3965547/71‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGttt < 1:2139750/71‑1 (MQ=255) aTGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGtt > 1:1655820/1‑70 (MQ=255) gCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGa > 1:592710/1‑51 (MQ=255) gCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGa > 1:3694785/1‑51 (MQ=255) gCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTAc > 1:1505101/1‑71 (MQ=255) cTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTAcc < 1:3808556/71‑1 (MQ=255) cTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTAcc > 1:3386455/1‑71 (MQ=255) cGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAg < 1:16879/63‑1 (MQ=255) gATCGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGaacc > 1:2304392/1‑48 (MQ=38) gATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCt < 1:2899788/69‑1 (MQ=255) gATCGTCAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGaacc > 1:7713/1‑48 (MQ=255) ttCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTa < 1:1510806/68‑1 (MQ=255) aTCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTAt < 1:3578378/70‑1 (MQ=255) tCGTGAAGGCAATCCGATTGAATCGCAGTTCAACtata > 1:4332511/1‑38 (MQ=255) tCGTGAAGGCAATCCGATTGAATCGCAGTTCAACtata > 1:1148640/1‑38 (MQ=255) tCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGa > 1:3613669/1‑71 (MQ=255) cGTGAAGGCACTCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACaa > 1:2655993/1‑54 (MQ=255) gTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTgg > 1:4400753/1‑42 (MQ=255) gTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTgg > 1:2137827/1‑42 (MQ=255) gTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGcac < 1:2963225/51‑1 (MQ=255) tGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGc < 1:1632245/45‑1 (MQ=255) tGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGttt < 1:3956167/60‑1 (MQ=255) gAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCt < 1:2622143/38‑1 (MQ=38) aaGGTAATCCGATTGAATCGCAGTTCAACTACACGCt < 1:4123140/37‑1 (MQ=255) aaGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGcc < 1:615370/70‑1 (MQ=255) aaGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGcc < 1:4034735/70‑1 (MQ=255) gCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATg < 1:2746143/71‑1 (MQ=255) cAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGa < 1:1973698/71‑1 (MQ=255) aaTCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGAc > 1:3638310/1‑71 (MQ=255) aTCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGttt < 1:3479266/52‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCGAGTTTAcc < 1:1805559/54‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTAcc < 1:1392310/54‑1 (MQ=255) ccGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTAc > 1:2799435/1‑52 (MQ=255) cGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGtt < 1:355442/48‑1 (MQ=255) ttGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGa > 1:343470/1‑54 (MQ=255) tGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTAcc > 1:2705404/1‑48 (MQ=255) tGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCt > 1:443049/1‑49 (MQ=255) tGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCggg > 1:2375255/1‑71 (MQ=255) tGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCggg > 1:665469/1‑71 (MQ=255) gAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTAc > 1:149636/1‑46 (MQ=255) tCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCa > 1:566808/1‑37 (MQ=255) tCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCa > 1:2442344/1‑70 (MQ=255) tCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCAt > 1:1303052/1‑71 (MQ=255) aGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTa < 1:538674/70‑1 (MQ=255) aGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTAt < 1:508135/71‑1 (MQ=255) ttCAACTATACGCTGGAAGCGGCACAATCCAGttt < 1:1898144/35‑1 (MQ=255) ttCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGAc < 1:866489/53‑1 (MQ=255) cAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTAtttt < 1:429904/70‑1 (MQ=255) cAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTAtttt > 1:680114/1‑70 (MQ=255) aaCTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGAccc > 1:3513228/1‑52 (MQ=255) aaCTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGAccc > 1:1442219/1‑52 (MQ=255) aaCTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTAttttt < 1:93189/70‑1 (MQ=255) aaCTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTAttttt < 1:183093/70‑1 (MQ=255) aaCTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTta < 1:1875210/71‑1 (MQ=255) aaCTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTta < 1:1999672/71‑1 (MQ=255) aaCTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTta < 1:876974/71‑1 (MQ=255) cTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGAt < 1:2498821/45‑1 (MQ=255) ataCGCTGGAAGCGGCACAATCCAGTTTACCTATGa > 1:2128857/1‑36 (MQ=255) ataCGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCg > 1:278598/1‑52 (MQ=255) ataCGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATg > 1:3003810/1‑71 (MQ=255) ataCGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATg > 1:77678/1‑71 (MQ=255) taCGCTGGAAGCGGCACAATCCAGTTTACCTATGCCGCCGATGACCCTGCGGGGCATGTATTTTTATATg > 1:3021666/1‑70 (MQ=255) taCGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGc < 1:2954030/50‑1 (MQ=255) | TCGGGCGAAAGGCGATGCGCTGGAGATGCTCGATCGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATTTTTATATG > minE/194423‑194561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |