| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,563,284 | C→T | 5.2% | N1097N (AAC→AAT) | evgS → | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,563,284 | 0 | C | T | 5.2% | 367.1 / 6.1 | 115 | N1097N (AAC→AAT) | evgS | hybrid sensory histidine kinase in two‑component regulatory system with EvgA |
| Reads supporting (aligned to +/- strand): ref base C (49/60); new base T (3/3); total (52/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
CCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGT > minE/1563215‑1563354 | ccAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAAc > 1:764116/1‑70 (MQ=255)ccAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACg < 1:1009963/71‑1 (MQ=255) cAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGa < 1:4346280/71‑1 (MQ=255) gcgcATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTAc > 1:3595551/1‑70 (MQ=255) gcgcATATTGCACCTCAGTATCGCCACCTTGATATAGAAGCCCTGAAAAATAATACGGCGAACGATCTAc > 1:2309807/1‑70 (MQ=255) cATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACaa > 1:2438373/1‑69 (MQ=255) tatTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACt < 1:940166/69‑1 (MQ=255) atTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGAt > 1:1067788/1‑71 (MQ=255) tGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATg < 1:2241519/70‑1 (MQ=255) tGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATg > 1:2038211/1‑70 (MQ=255) tGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATg < 1:198602/70‑1 (MQ=255) cTCAGTATCGCCACCTTGATATCGAGGCCCTGAAGAATAATACGGCGAATGATCt > 1:1007184/1‑55 (MQ=255) cTCAGTATCGCCACCTTGATATCGAGGCCCTGAAGAATAATACGGCGAATGATCt > 1:3599040/1‑55 (MQ=255) cTCAGTATCGCCACCTTGATATCGAGGCCCTGAAGAATAATACGGCGAATGATCt > 1:3782046/1‑55 (MQ=255) cTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACg < 1:5038/51‑1 (MQ=255) tCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGAt > 1:4582072/1‑52 (MQ=255) cAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGAt > 1:2996509/1‑51 (MQ=255) tATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATtct < 1:1113211/71‑1 (MQ=255) aTCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATtct > 1:4282495/1‑70 (MQ=255) tCGCCACCTTGATATCGAGGCCCTGAAGAATAATACGGCGAATGATCTACAACTGATGCAGGAGATtctc < 1:4146222/70‑1 (MQ=255) tCGCCACCTTGATATCGAGGCCCTGAAGAATAATACGGCGAATGATCTACAACTGATGCAGGAGATtctc < 1:1181613/70‑1 (MQ=255) tCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATc < 1:347960/47‑1 (MQ=255) tCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCa > 1:1159447/1‑71 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATc < 1:4117603/45‑1 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt > 1:388739/1‑70 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt < 1:3904116/70‑1 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt < 1:1754840/70‑1 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt > 1:3180094/1‑70 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt > 1:2936955/1‑70 (MQ=255) gCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATg < 1:4544322/71‑1 (MQ=255) ccACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGa < 1:1273185/71‑1 (MQ=255) ccACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGa > 1:1105290/1‑71 (MQ=255) cACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGAc > 1:337009/1‑71 (MQ=255) aCCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGAt > 1:3129396/1‑41 (MQ=255) aCCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACt < 1:4297112/71‑1 (MQ=255) ccTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTAc < 1:118119/44‑1 (MQ=255) cTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGga < 1:319476/56‑1 (MQ=255) cTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACttt < 1:1054867/71‑1 (MQ=255) cTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACttt < 1:3704783/71‑1 (MQ=255) cTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACttt < 1:1248196/71‑1 (MQ=255) cTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACttt < 1:2033620/71‑1 (MQ=255) ttGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt < 1:3078251/64‑1 (MQ=255) atatCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTGTCATGACTTTcc < 1:1846891/69‑1 (MQ=255) tCGAAGCCCTGAAAAATAATACGGCGAACGATCTACa < 1:1166197/37‑1 (MQ=255) tCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATg < 1:1703425/44‑1 (MQ=255) tCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATg < 1:3584050/44‑1 (MQ=255) tCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCAt < 1:253691/58‑1 (MQ=255) gAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATg > 1:4370306/1‑70 (MQ=255) gAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGa < 1:1239637/71‑1 (MQ=255) gAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGa < 1:2380899/71‑1 (MQ=255) gAAGCCCTGAAAAATAATAC‑‑CGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGaaa > 1:3373841/1‑71 (MQ=255) gCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGa < 1:4106690/68‑1 (MQ=255) gCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAAc < 1:261851/71‑1 (MQ=255) ccTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTACAGCATGAAACg > 1:4366224/1‑70 (MQ=255) cTGAAAAATAATACGGCGAACGATCTACAACTGAt > 1:4024437/1‑35 (MQ=255) cTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGgaga < 1:160989/43‑1 (MQ=255) cTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAg < 1:3310004/60‑1 (MQ=255) cTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCa > 1:4687141/1‑71 (MQ=255) cTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCa < 1:1646211/71‑1 (MQ=255) cTGAAAAATAATACGGCGAACGATCTACAACTGATGAAGgaga < 1:4553551/43‑1 (MQ=255) tGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATg < 1:4089043/50‑1 (MQ=255) tGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGa < 1:4330890/64‑1 (MQ=255) tGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCa > 1:805668/1‑70 (MQ=255) tGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCa > 1:615942/1‑70 (MQ=255) gAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGAc > 1:887260/1‑51 (MQ=255) gAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGAc > 1:881789/1‑51 (MQ=255) gAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCAt > 1:3427518/1‑70 (MQ=255) gAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCAt > 1:3405432/1‑70 (MQ=255) gAAAAATAATACGGCGAACGATCTACAACTGATGAAGGAGATTCTCATGACTTTCCAGCATGAAACGCAt > 1:2574657/1‑70 (MQ=255) gaaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATa > 1:224607/2‑70 (MQ=255) aaaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGa > 1:2840818/1‑49 (MQ=255) aaaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCAt < 1:505128/69‑1 (MQ=255) aaaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATaa < 1:3094346/71‑1 (MQ=255) aaaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATa > 1:2438355/1‑70 (MQ=255) aaaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATa > 1:1689216/1‑70 (MQ=255) aaaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATa > 1:3997809/1‑70 (MQ=255) aaaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATa > 1:1093970/1‑70 (MQ=255) aaaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATa < 1:3645823/70‑1 (MQ=255) aaaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATa > 1:740332/1‑70 (MQ=255) aaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGa > 1:1965395/1‑48 (MQ=255) aaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGa < 1:1135703/61‑1 (MQ=255) aaaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATa > 1:3544503/1‑69 (MQ=255) aaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTGTCATGACTTTCCAGCATGAAACGCATa > 1:1303381/1‑68 (MQ=255) aaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATa > 1:754473/1‑68 (MQ=255) aaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAg < 1:3011829/71‑1 (MQ=255) aaaTAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAg < 1:1345086/71‑1 (MQ=255) aataatACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGc < 1:1646795/55‑1 (MQ=255) taataCGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATc > 1:2380134/1‑71 (MQ=255) ataCGGCGAACGATCTACAACTGATGCAGGAGATtct > 1:944790/1‑37 (MQ=255) ataCGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATa < 1:3631391/63‑1 (MQ=255) taCGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCAAGCATGAAACGCAT‑AAGATCTAc < 1:1280343/70‑1 (MQ=255) aCGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGc < 1:2525560/49‑1 (MQ=255) gCGAACGATCTACAACTGATGCAGGAGATTCTCATGAc > 1:1981980/1‑38 (MQ=255) cGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAg < 1:910427/60‑1 (MQ=255) gAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAg < 1:2856817/43‑1 (MQ=255) gAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTg < 1:656199/71‑1 (MQ=255) aaCGATCTACAACTGATTCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGc < 1:2226457/71‑1 (MQ=255) aaCGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGa < 1:3731549/47‑1 (MQ=255) aaCGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGa < 1:3783095/47‑1 (MQ=255) cGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGt > 1:2767382/1‑71 (MQ=255) | CCAAGTTGCGCATATTGCACCTCAGTATCGCCACCTTGATATCGAAGCCCTGAAAAATAATACGGCGAACGATCTACAACTGATGCAGGAGATTCTCATGACTTTCCAGCATGAAACGCATAAAGATCTACCCGCTGCGT > minE/1563215‑1563354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |