| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,568,329 | A→G | 5.5% | L333L (TTG→CTG) | mntH ← | manganese/divalent cation transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,568,329 | 0 | A | G | 5.5% | 312.4 / 7.1 | 114 | L333L (TTG→CTG) | mntH | manganese/divalent cation transporter |
| Reads supporting (aligned to +/- strand): ref base A (47/60); new base G (3/3); total (51/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
AACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACC > minE/1568259‑1568393 | aaCAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCaa > 1:1949485/1‑71 (MQ=255)aaCAGTACCTGACTCATAACCAGAATCCGTGTCCGATCTAATCCCATCAGAATGACAATAAATGACGGCaa > 1:2185113/1‑71 (MQ=255) aCAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCaa < 1:1405311/70‑1 (MQ=255) aCAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAAc < 1:2669298/71‑1 (MQ=255) gcaCCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACGATAAATGACGGCAGCATg > 1:3467979/3‑71 (MQ=255) gTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATg < 1:68188/71‑1 (MQ=255) gTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATg < 1:3608310/71‑1 (MQ=255) tGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGAc > 1:2295000/1‑71 (MQ=255) gACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAAGATGGTGACt > 1:4266855/1‑71 (MQ=255) gACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACt > 1:4631640/1‑71 (MQ=255) aCTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACt < 1:78638/70‑1 (MQ=255) aCTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAAAATGGTGACTg < 1:4068730/71‑1 (MQ=255) cTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTg < 1:1072022/70‑1 (MQ=255) cTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTg > 1:1735957/1‑70 (MQ=255) tCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTg < 1:4237062/69‑1 (MQ=255) aTAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACGATAAATGACGGCAGCATGGTGACTGTacg < 1:4145472/71‑1 (MQ=255) aTAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacg < 1:1972489/71‑1 (MQ=255) aTAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTac < 1:102029/70‑1 (MQ=255) tAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCaa < 1:1000414/55‑1 (MQ=255) tAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCaa < 1:1425607/55‑1 (MQ=255) tAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacga > 1:3142008/1‑71 (MQ=255) aaCCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacgac > 1:1268643/1‑71 (MQ=255) aaCCAGAATACGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacga > 1:1817418/1‑70 (MQ=255) aCCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacgacg < 1:488269/71‑1 (MQ=255) cAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACAt > 1:1691415/1‑54 (MQ=255) aGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCATCATGGTGACTGTACGACGCAc > 1:3198360/1‑71 (MQ=255) gAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTg < 1:4608138/60‑1 (MQ=255) aaTCCGTGTCGGATCTAATCCCATCAGAATGACGATAAATGACGGCAGCATGGTGACTGTacg < 1:4179255/63‑1 (MQ=255) aaTCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACa < 1:276329/50‑1 (MQ=255) aaTCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTac < 1:1954519/62‑1 (MQ=255) aaTCCGTGTCGGATCGAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTac < 1:2938959/62‑1 (MQ=255) ccGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGa > 1:3159372/1‑53 (MQ=255) cGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAg > 1:1297703/1‑71 (MQ=255) gtgtCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGt < 1:1952108/49‑1 (MQ=255) gtgtCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGc < 1:186277/71‑1 (MQ=255) tgtCGTATCTAATCCAATCAGAATGACAATAAATGACGGCAACATg < 1:3478555/46‑1 (MQ=37) tgtCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCcac < 1:617441/67‑1 (MQ=255) tgtCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCg < 1:2392013/71‑1 (MQ=255) tgtCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCg < 1:2448889/71‑1 (MQ=255) tCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGt > 1:1588870/1‑46 (MQ=255) tCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacga < 1:1945141/56‑1 (MQ=255) tCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCggg < 1:2901957/71‑1 (MQ=255) cGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGa > 1:2268712/1‑71 (MQ=255) ggATCTAATCCCATCAGAATGACAATAAATGACGTCAACAt > 1:3112294/1‑41 (MQ=255) ggATCTAATCCCATCAGAATGACAATAAATGACGGCAAc > 1:1687538/1‑39 (MQ=255) ggATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGa < 1:875551/70‑1 (MQ=255) ggATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGa < 1:2652847/70‑1 (MQ=255) ggATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGa > 1:2303363/1‑70 (MQ=255) aTCTAATCCCATCAGAATGACAATAAATGACGGCAAc < 1:872701/37‑1 (MQ=255) cTAATCCCATCAGAATGACGATAAATGACGGCAGCATGGTGACTGTACGACGCAccc > 1:3900890/1‑57 (MQ=38) cTAATCCCATCAGAATGACGATAAATGACGGCAGCATGGTGACTGTACGACGCAccc > 1:2884876/1‑57 (MQ=38) cTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTa > 1:1826545/1‑47 (MQ=255) cTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCa > 1:635352/1‑54 (MQ=255) tAATCCCATCAGAATGACAATAAATGACGGCAACa < 1:2170702/35‑1 (MQ=255) tAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACt < 1:633236/43‑1 (MQ=255) tAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACt < 1:56063/43‑1 (MQ=255) tAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACt < 1:4195118/43‑1 (MQ=255) tCCCATCATAATGACAATAAATGACGGCAACATGGt < 1:333423/36‑1 (MQ=255) tCCCATCAGAATGACAATAAATGACGGCAACATGGt < 1:1267697/36‑1 (MQ=255) tCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTacga > 1:436541/1‑46 (MQ=255) cccATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCcac > 1:4335068/1‑54 (MQ=255) cccATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGc > 1:2181775/1‑70 (MQ=255) cccATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGc > 1:3358239/1‑70 (MQ=255) ccATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCg < 1:3555313/70‑1 (MQ=255) ccATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCg < 1:2043110/70‑1 (MQ=255) ccATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGa < 1:1738546/71‑1 (MQ=255) ccATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGa < 1:1083618/71‑1 (MQ=255) cATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGaa < 1:1290262/71‑1 (MQ=255) aTCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCgg < 1:1771259/56‑1 (MQ=255) aTCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAAt < 1:3743097/71‑1 (MQ=255) tCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGa < 1:2677028/62‑1 (MQ=255) tCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGaa < 1:1203026/69‑1 (MQ=255) tCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAAt > 1:1611417/1‑70 (MQ=255) tCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATg < 1:3774612/71‑1 (MQ=255) aGAATGACAATAAATGACGGCAACATGGTGACTGTa > 1:1848527/1‑36 (MQ=255) aGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGaaa > 1:486820/1‑62 (MQ=255) aGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATg > 1:2835131/1‑69 (MQ=255) aGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATg > 1:2125275/1‑69 (MQ=255) aGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGaa < 1:3911814/71‑1 (MQ=255) gAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAg > 1:2334537/1‑71 (MQ=255) tGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGccc > 1:388289/1‑71 (MQ=255) aCAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTg < 1:3490651/71‑1 (MQ=255) cAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTg > 1:2884009/1‑70 (MQ=255) aaTAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCg < 1:832072/57‑1 (MQ=255) aaTAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCg < 1:2297455/57‑1 (MQ=255) aaTAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCa < 1:3278205/71‑1 (MQ=255) aaTAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCa < 1:4308934/71‑1 (MQ=255) aTAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGa < 1:2981091/61‑1 (MQ=255) tAAATGACGGCAACATGGTGACTTTACGACGCACCCACAGCGGGATATGAAAGCg < 1:1450543/55‑1 (MQ=255) tAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCAt > 1:3410902/1‑70 (MQ=255) aaaTGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGaaa > 1:1375191/1‑51 (MQ=255) aaTGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGaaa > 1:2184314/1‑50 (MQ=255) aTGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAAt < 1:258015/55‑1 (MQ=255) aTGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATcacc < 1:2583095/71‑1 (MQ=255) tGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGa > 1:179459/1‑41 (MQ=255) tGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATcacca < 1:2144218/71‑1 (MQ=255) aaCATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCaccacc > 1:1883033/1‑66 (MQ=255) aCATGGTGACTGTACGACGCACCCACAGCGGGATATg < 1:3593759/37‑1 (MQ=255) aCATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATcacca < 1:1279169/63‑1 (MQ=255) aCATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATc > 1:3726994/1‑59 (MQ=255) | AACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACC > minE/1568259‑1568393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |