| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,590,902 | A→G | 45.9% | V32V (GTA→GTG) | yfeS → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,590,902 | 0 | A | G | 45.9% | 9.9 / 95.5 | 73 | V32V (GTA→GTG) | yfeS | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (13/26); new base G (12/21); total (25/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAA > minE/1590834‑1590971 | gAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggtggg > 1:4209920/1‑71 (MQ=255)gAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggtagg > 1:4467478/1‑71 (MQ=255) aaaaaTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggtagg < 1:3579489/70‑1 (MQ=255) aaaaTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggtggg > 1:3271782/1‑69 (MQ=255) aaaaTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggtggg > 1:4502171/1‑69 (MQ=255) aaaaTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggtaggt < 1:3449448/70‑1 (MQ=255) aaaTCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGgtgggtag < 1:4227057/71‑1 (MQ=255) tCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTAt < 1:3950855/71‑1 (MQ=255) cGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTAtt > 1:4176437/1‑71 (MQ=255) aTAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTgg > 1:4084111/1‑70 (MQ=255) aaTTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTaaa > 1:1646546/1‑71 (MQ=255) aTTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAAt > 1:2321281/1‑71 (MQ=255) ttGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTc > 1:4015058/1‑70 (MQ=255) tggtggATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTc < 1:3634587/69‑1 (MQ=255) ggtggATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCa < 1:924084/70‑1 (MQ=255) ggtggATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCa < 1:2622406/70‑1 (MQ=255) gtggATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAg < 1:1643976/70‑1 (MQ=255) tggATCGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGgtagt < 1:3577136/54‑1 (MQ=255) tggATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCa < 1:590634/68‑1 (MQ=255) tggATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGgtagt < 1:501285/54‑1 (MQ=255) gATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACaa < 1:2621989/71‑1 (MQ=255) gATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACaa < 1:2526369/71‑1 (MQ=255) gATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACaa < 1:2352675/71‑1 (MQ=255) gATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACaa < 1:1624782/71‑1 (MQ=255) aTAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACaaa < 1:1521097/71‑1 (MQ=255) aTAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACaa > 1:3326123/1‑70 (MQ=255) tAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACaaaa < 1:69325/71‑1 (MQ=255) tAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACaaaa < 1:3702172/71‑1 (MQ=255) tAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACaaaa < 1:1376950/71‑1 (MQ=255) tAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACaaaa < 1:4457593/71‑1 (MQ=255) aGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACaa > 1:2116697/1‑68 (MQ=255) gATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAag < 1:3403504/70‑1 (MQ=255) aTTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACaaaa < 1:1154887/68‑1 (MQ=255) aTTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAagag < 1:3758853/71‑1 (MQ=255) aTTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAagag < 1:3275689/71‑1 (MQ=255) aTTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAagag > 1:1427849/1‑71 (MQ=255) ttACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggtggg < 1:974542/42‑1 (MQ=255) ttACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACaaaa < 1:741056/67‑1 (MQ=255) tACGAAGGGGATAGTTTAGCTGTCAACTATGGCAaggtagg > 1:1282880/1‑41 (MQ=255) aCGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCa > 1:2474176/1‑58 (MQ=255) aCGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACaa > 1:371800/1‑63 (MQ=255) aCGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACa > 1:91967/1‑62 (MQ=255) cGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTa < 1:1556951/50‑1 (MQ=255) cGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTc < 1:4646176/70‑1 (MQ=255) gAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTa < 1:3581872/49‑1 (MQ=255) gAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGa > 1:3649960/1‑71 (MQ=255) gAAGGGGATAGTTTAGCTCTCAACTATGGCAAGGTGGGTAGTATTGGTa < 1:2058844/49‑1 (MQ=255) aaGGGGATAGTTTAGCTGTCAAATATGGCAAGGTTTGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGAt < 1:2612532/71‑1 (MQ=255) ggggATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACaaa > 1:3784249/1‑59 (MQ=255) gATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGt < 1:126258/42‑1 (MQ=255) gATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATg > 1:2763074/1‑70 (MQ=255) aTAGTTTAGCTGTCAATTATGGCTTGGTAGGTAGTATTTGTa < 1:3364096/42‑1 (MQ=255) tAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaat < 1:454959/71‑2 (MQ=255) tAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaag < 1:1494811/71‑1 (MQ=255) tAGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaag < 1:4477492/71‑1 (MQ=255) tAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCa < 1:4674221/48‑1 (MQ=255) tAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCa < 1:3920091/48‑1 (MQ=255) tAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaag < 1:115241/71‑1 (MQ=255) aGTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaag > 1:3252030/1‑70 (MQ=255) gTTTAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGAt < 1:361394/62‑1 (MQ=255) ttAGCTGTCAACTATGGCAAGGTGGGTAGTATTgg > 1:4545354/1‑35 (MQ=255) ttAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaagaa > 1:3460046/1‑69 (MQ=255) tAGCTGTCAACTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATaa > 1:240854/1‑61 (MQ=255) tAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATa > 1:1900422/1‑60 (MQ=255) gCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAgtg < 1:1709845/71‑1 (MQ=255) tCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAAc < 1:4672833/63‑1 (MQ=255) aaCTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAAc < 1:3245803/61‑1 (MQ=255) aaCTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGa < 1:459473/70‑1 (MQ=255) aaCTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGa < 1:3233615/70‑1 (MQ=255) cTATGGCAAGGTGGGTAGTATTGGTAAATTCCAGACaaaa < 1:2049410/40‑1 (MQ=255) cTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaa > 1:1261745/1‑55 (MQ=255) cTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaag < 1:2686660/71‑1 (MQ=255) tATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAaga > 1:1978274/1‑41 (MQ=255) tATGGCAAGGTAGGTAGTATAGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTCTCTGAaag > 1:3562681/1‑70 (MQ=255) aTGGCAAGGTGGGTAGTATTGGTAAATTCCAGACaaaa > 1:1308384/1‑38 (MQ=255) aTGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAAc > 1:1419550/1‑57 (MQ=255) tGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaa < 1:236505/52‑1 (MQ=255) gCAAGGTGGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGc < 1:3905329/70‑1 (MQ=255) gCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGcc < 1:2478986/71‑1 (MQ=255) gCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGc < 1:1621966/70‑1 (MQ=255) cAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaagaag > 1:4136706/1‑68 (MQ=255) cAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGcc < 1:2712810/70‑1 (MQ=255) aggtgggtAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAg > 1:2162952/1‑70 (MQ=255) aggtgggtAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAg > 1:3219947/1‑70 (MQ=255) ggtgggtaGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGt > 1:1589186/1‑70 (MQ=255) ggtgggtaGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTa < 1:2748029/71‑1 (MQ=255) gtaggtagTATTGGTAAATTCCAGACAAAAGAGTTCGATAATga < 1:4257264/44‑1 (MQ=255) gtaggtagTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaag < 1:3113942/61‑1 (MQ=255) gtaggtagTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaag < 1:1012731/61‑1 (MQ=255) gtaggtagTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTa > 1:3647574/1‑70 (MQ=255) taggtagTATTGGTAAATTCCAGACAAAAGAGTTCGATAATgaagaa > 1:680207/1‑47 (MQ=255) taggtagTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAaag < 1:4036019/60‑1 (MQ=255) taggtagTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTaaa > 1:2171084/1‑71 (MQ=255) | GAAAAATCGAATAAATTTTGGTGGATAGATTACGAAGGGGATAGTTTAGCTGTCAACTATGGCAAGGTAGGTAGTATTGGTAAATTCCAGACAAAAGAGTTCGATAATGAAGAACAGTGTCTGAAAGAAGCCAGTAAA > minE/1590834‑1590971 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |