| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,730,964 | G→A | 6.3% | R91R (CGC→CGT) | yfhB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,730,964 | 0 | G | A | 6.3% | 345.0 / 8.5 | 111 | R91R (CGC→CGT) | yfhB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (42/61); new base A (2/5); total (45/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
TATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCC > minE/1730894‑1731032 | tatCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTcg > 1:1003486/1‑71 (MQ=255) tCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTcg < 1:3366668/69‑1 (MQ=255) aGCATCGGAACTT‑ACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGaa < 1:3433946/70‑1 (MQ=255) tCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCa < 1:260466/71‑1 (MQ=255) ggAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAgc > 1:2698146/1‑71 (MQ=255) ggAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAgc > 1:4067290/1‑71 (MQ=255) aaCTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAgcg > 1:3049757/1‑70 (MQ=255) aaCTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAgcg > 1:3713922/1‑70 (MQ=255) ttAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTcgcg < 1:67263/58‑1 (MQ=255) tAACAGGTAGGTTGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAAc < 1:1662098/61‑1 (MQ=255) aaCAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGaa > 1:1633769/1‑58 (MQ=255) aaCAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGa < 1:195768/70‑1 (MQ=255) aCAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGaa < 1:396533/70‑1 (MQ=255) cAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCAACGTACGAAAt < 1:2632336/71‑1 (MQ=255) cAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCAACGTACGAAAt < 1:4559766/71‑1 (MQ=255) cAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCAACGTACGAAAt < 1:4377446/71‑1 (MQ=255) aggtaggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATc < 1:3188806/71‑1 (MQ=255) ggtaggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCg < 1:3968556/71‑1 (MQ=255) aggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCAACGTACGAAAt < 1:1983355/66‑1 (MQ=255) aggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGT‑‑CGAAACCAGCGCACGAAATCGGCCTg > 1:1793219/1‑71 (MQ=255) gtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACg < 1:1758789/60‑1 (MQ=255) gtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACg < 1:3938748/60‑1 (MQ=255) gtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGaa < 1:1393675/62‑1 (MQ=255) gtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGc < 1:4449003/68‑1 (MQ=255) tggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:3823864/71‑1 (MQ=255) tggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:265223/71‑1 (MQ=255) tggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:1640169/71‑1 (MQ=255) tggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:4404904/71‑1 (MQ=255) gtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCa < 1:608872/70‑1 (MQ=255) ttAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAAc < 1:4476514/71‑1 (MQ=255) ttAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAAc > 1:2308828/1‑71 (MQ=255) aaTCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCa > 1:1225120/1‑47 (MQ=255) aaTCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTG‑AACGTc > 1:4570563/1‑71 (MQ=255) aTCGCTCCTGACCCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGa > 1:2092910/1‑54 (MQ=255) aTCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAAt > 1:2235539/1‑57 (MQ=255) aTCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTc > 1:2230014/1‑71 (MQ=255) tCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAgcg > 1:2397865/1‑48 (MQ=255) tCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCt < 1:1487056/62‑1 (MQ=255) tCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:369365/64‑1 (MQ=255) tCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:3371368/64‑1 (MQ=255) cGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAAc > 1:579856/1‑66 (MQ=255) gCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTg > 1:2076465/1‑70 (MQ=255) ccTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCa > 1:2751103/1‑45 (MQ=255) cTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAAc < 1:2906980/37‑1 (MQ=255) cTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaa < 1:3986754/69‑1 (MQ=255) gAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGt > 1:3702402/1‑71 (MQ=255) cAGCGGATAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCtt > 1:525245/1‑71 (MQ=255) cAGCGGAAAGGTGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCtt > 1:3747770/1‑71 (MQ=255) cAGCGGAAAGGCGGTACCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCtt > 1:2356718/1‑71 (MQ=255) cAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCtt > 1:4205628/1‑71 (MQ=255) gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAAt < 1:458470/42‑1 (MQ=255) gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGc > 1:3109011/1‑67 (MQ=255) gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCg < 1:631811/71‑1 (MQ=255) gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCg < 1:1307877/71‑1 (MQ=255) gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTG‑AACGTCTGTAAACGTGCTTCGc < 1:52963/71‑1 (MQ=255) ggAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt < 1:112759/71‑1 (MQ=255) gAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCaa > 1:4394517/1‑49 (MQ=255) gAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtg < 1:457597/71‑1 (MQ=255) gAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtg < 1:2563332/71‑1 (MQ=255) gAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtg > 1:597706/1‑71 (MQ=255) aaaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGTCCTGCAACGTCTGTa < 1:2282389/56‑1 (MQ=255) aaaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTa < 1:4199133/56‑1 (MQ=255) aaaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtgt < 1:2522904/71‑1 (MQ=255) aaaGGCGGTAACATTGTCACGAAACCAACGTACGAAATCGGCCTGCAACGCCTGCAAAc > 1:1662872/1‑59 (MQ=255) aaaGGCGGTAACATTGTCACGAAACCAACGTACGAAATCGGCCTGCAACGCCTGCAAAc > 1:1365486/1‑59 (MQ=255) aaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAAc > 1:4430056/1‑58 (MQ=255) aGGCGTTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGa > 1:2169385/1‑71 (MQ=255) aGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaaa < 1:2181783/56‑1 (MQ=255) gCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGAcc < 1:3361439/71‑1 (MQ=255) gCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGAcc < 1:3355850/71‑1 (MQ=255) ggTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaa < 1:1405525/71‑1 (MQ=255) ggTAACATTGTCGCGAAACCAGCCCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaa < 1:419404/71‑1 (MQ=255) gTAACATTGTCGCGAACCCAGCGCACGAAATCGGCCTGCAACGTCTGtt < 1:1230132/49‑2 (MQ=255) gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGt < 1:3420914/44‑1 (MQ=255) gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt < 1:2316440/62‑1 (MQ=255) gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt < 1:2310990/62‑1 (MQ=255) tAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa < 1:648364/71‑1 (MQ=255) tAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa < 1:2142580/71‑1 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:643913/37‑1 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:449258/37‑1 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGt > 1:4223662/1‑42 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaaa > 1:3270307/1‑49 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaa > 1:4087585/1‑48 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAg > 1:771745/1‑71 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAg > 1:2895023/1‑71 (MQ=255) aCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAg < 1:1433584/70‑1 (MQ=255) aCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGt > 1:2285043/1‑71 (MQ=255) cATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtgt < 1:4094285/60‑1 (MQ=255) aTTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaa > 1:4403098/1‑65 (MQ=255) ttGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTa < 1:3870381/43‑1 (MQ=255) ttGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGt > 1:1904304/1‑68 (MQ=255) ttGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCa > 1:2740419/1‑71 (MQ=255) gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaa < 1:25514/42‑1 (MQ=255) gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAAc > 1:2213488/1‑44 (MQ=255) gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtg > 1:2040178/1‑55 (MQ=255) gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCAcc > 1:4170451/1‑71 (MQ=255) gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCAcc > 1:3162841/1‑71 (MQ=255) tCGCGAAACCATCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCAccc < 1:1322238/71‑1 (MQ=255) gcgAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGAcc > 1:636868/1‑57 (MQ=255) gcgAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa > 1:1423753/1‑61 (MQ=255) | TATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCC > minE/1730894‑1731032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |