Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,730,973 G→A 5.2% R88R (CGC→CGT yfhB ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,730,9730GA 5.2% 363.9 / 4.8 115R88R (CGC→CGTyfhBconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (45/63);  new base A (2/4);  total (47/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCA  >  minE/1730904‑1731043
                                                                     |                                                                      
ggAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAgc                                                                       >  1:2698146/1‑71 (MQ=255)
ggAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAgc                                                                       >  1:4067290/1‑71 (MQ=255)
  aaCTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAgcg                                                                      >  1:3049757/1‑70 (MQ=255)
  aaCTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAgcg                                                                      >  1:3713922/1‑70 (MQ=255)
        aCAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGaa                                                                <  1:396533/70‑1 (MQ=255)
        aCAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGa                                                                 <  1:3354926/69‑1 (MQ=255)
         cAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCAACGTACGAAAt                                                              <  1:2632336/71‑1 (MQ=255)
         cAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCAACGTACGAAAt                                                              <  1:4559766/71‑1 (MQ=255)
         cAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCAACGTACGAAAt                                                              <  1:4377446/71‑1 (MQ=255)
          aggtaggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATc                                                             <  1:3188806/71‑1 (MQ=255)
           ggtaggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCg                                                            <  1:3968556/71‑1 (MQ=255)
            gtaggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCg                                                            <  1:1869840/70‑1 (MQ=255)
              aggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCAACGTACGAAAt                                                              <  1:1983355/66‑1 (MQ=255)
              aggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGT‑‑CGAAACCAGCGCACGAAATCGGCCTg                                                       >  1:1793219/1‑71 (MQ=255)
                gtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACg                                                                  <  1:3938748/60‑1 (MQ=255)
                gtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGaa                                                                <  1:1393675/62‑1 (MQ=255)
                gtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGc                                                          <  1:4449003/68‑1 (MQ=255)
                 tggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc                                                      <  1:1640169/71‑1 (MQ=255)
                 tggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc                                                      <  1:3823864/71‑1 (MQ=255)
                 tggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc                                                      <  1:4404904/71‑1 (MQ=255)
                   gtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCa                                                     <  1:608872/70‑1 (MQ=255)
                      aaTCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGAACGTc                                                >  1:4570563/1‑71 (MQ=255)
                       aTCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAAt                                                              >  1:2235539/1‑57 (MQ=255)
                       aTCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTc                                                >  1:2230014/1‑71 (MQ=255)
                        tCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAgcg                                                                      >  1:2397865/1‑48 (MQ=255)
                        tCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc                                                      <  1:3371368/64‑1 (MQ=255)
                         cGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAAc                                                   >  1:579856/1‑66 (MQ=255)
                         cGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTg                                              >  1:522239/1‑71 (MQ=255)
                          gCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAAt                                                              >  1:1592637/1‑54 (MQ=255)
                          gCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTg                                              >  1:2076465/1‑70 (MQ=255)
                             ccTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCa                                                                    >  1:2751103/1‑45 (MQ=255)
                                gAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGt                                       >  1:3702402/1‑71 (MQ=255)
                                 aaCCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACg                                        <  1:4089535/69‑1 (MQ=255)
                                    cAGCGGATAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCtt                                   >  1:525245/1‑71 (MQ=255)
                                    cAGCGGAAAGGTGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCtt                                   >  1:3747770/1‑71 (MQ=255)
                                    cAGCGGAAAGGCGGTACCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCtt                                   >  1:2356718/1‑71 (MQ=255)
                                    cAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCtt                                   >  1:4205628/1‑71 (MQ=255)
                                      gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAAt                                                              <  1:458470/42‑1 (MQ=255)
                                      gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCg                                 <  1:631811/71‑1 (MQ=255)
                                      gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCg                                 <  1:1307877/71‑1 (MQ=255)
                                      gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGAACGTCTGTAAACGTGCTTCGc                                <  1:52963/71‑1 (MQ=255)
                                        ggAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt                               <  1:112759/71‑1 (MQ=255)
                                        ggAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt                               <  1:2648884/71‑1 (MQ=255)
                                         gAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtg                              <  1:2563332/71‑1 (MQ=255)
                                         gAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtg                              >  1:597706/1‑71 (MQ=255)
                                          aaaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGTCCTGCAACGTCTGTa                                            <  1:2282389/56‑1 (MQ=255)
                                          aaaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTa                                            <  1:4199133/56‑1 (MQ=255)
                                          aaaGGCGGTAACATTGTCACGAAACCAACGTACGAAATCGGCCTGCAACGCCTGCAAAc                                         >  1:1662872/1‑59 (MQ=255)
                                           aaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAAc                                         >  1:4430056/1‑58 (MQ=255)
                                           aaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtgtg                            <  1:393771/71‑1 (MQ=255)
                                            aGGCGTTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGa                           >  1:2169385/1‑71 (MQ=255)
                                            aGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaaa                                          <  1:2181783/56‑1 (MQ=255)
                                              gCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGAcc                         <  1:3361439/71‑1 (MQ=255)
                                                ggTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaa                       <  1:2097716/71‑1 (MQ=255)
                                                ggTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaa                       <  1:1405525/71‑1 (MQ=255)
                                                 gTAACATTGTCGCGAACCCAGCGCACGAAATCGGCCTGCAACGTCTGtt                                            <  1:1230132/49‑2 (MQ=255)
                                                 gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGt                                                 <  1:3420914/44‑1 (MQ=255)
                                                 gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaa                                           <  1:3577299/50‑1 (MQ=255)
                                                 gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTctct                               <  1:1348043/62‑1 (MQ=255)
                                                 gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt                               <  1:2310990/62‑1 (MQ=255)
                                                 gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt                               <  1:2316440/62‑1 (MQ=255)
                                                 gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaa                       <  1:226426/70‑1 (MQ=255)
                                                 gTAACATTGTCGCGAAACCAGCGCACAAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaa                      <  1:2370295/71‑1 (MQ=255)
                                                  tAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa                     <  1:648364/71‑1 (MQ=255)
                                                  tAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa                     <  1:2142580/71‑1 (MQ=255)
                                                   aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc                                                      <  1:449258/37‑1 (MQ=255)
                                                   aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc                                                      <  1:643913/37‑1 (MQ=255)
                                                   aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGt                                                 >  1:4223662/1‑42 (MQ=255)
                                                   aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaaa                                          >  1:3270307/1‑49 (MQ=255)
                                                   aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaa                                           >  1:4087585/1‑48 (MQ=255)
                                                   aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAg                    >  1:2895023/1‑71 (MQ=255)
                                                   aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAg                    >  1:771745/1‑71 (MQ=255)
                                                    aCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAg                    <  1:1433584/70‑1 (MQ=255)
                                                    aCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGt                   >  1:2285043/1‑71 (MQ=255)
                                                     cATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtgt                             <  1:4094285/60‑1 (MQ=255)
                                                      aTTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaa                       >  1:4403098/1‑65 (MQ=255)
                                                       ttGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTa                                            <  1:3870381/43‑1 (MQ=255)
                                                       ttGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGt                   >  1:1904304/1‑68 (MQ=255)
                                                       ttGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCa                >  1:2740419/1‑71 (MQ=255)
                                                         gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaa                                           <  1:25514/42‑1 (MQ=255)
                                                         gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtg                              >  1:2040178/1‑55 (MQ=255)
                                                         gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCAcc              >  1:4170451/1‑71 (MQ=255)
                                                         gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCAcc              >  1:635963/1‑71 (MQ=255)
                                                         gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCAcc              >  1:3162841/1‑71 (MQ=255)
                                                          tCGCGAAACCATCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCAccc             <  1:1322238/71‑1 (MQ=255)
                                                            gcgAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa                     >  1:4259676/1‑61 (MQ=255)
                                                            gcgAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa                     >  1:1423753/1‑61 (MQ=255)
                                                             cgAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCca          <  1:2107634/71‑1 (MQ=255)
                                                              gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaa                      <  1:4503128/58‑1 (MQ=255)
                                                              gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCca          >  1:172215/1‑70 (MQ=255)
                                                              gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCca          >  1:1443659/1‑70 (MQ=255)
                                                              gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCca          >  1:1940133/1‑70 (MQ=255)
                                                              gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTACCAAAAGTg                  <  1:159598/61‑1 (MQ=255)
                                                              gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGGGACCAAAAGTGCACCCCca          >  1:177574/1‑70 (MQ=255)
                                                                aaCCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACg                                        <  1:343484/38‑1 (MQ=255)
                                                                aaCCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCca          >  1:2073484/1‑68 (MQ=255)
                                                                aaCCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAg       <  1:514049/71‑1 (MQ=255)
                                                                aaCCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAg       <  1:4480378/71‑1 (MQ=255)
                                                                   cAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAg    <  1:774149/71‑1 (MQ=255)
                                                                     gcgcACGAAATCGTCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCa  <  1:3131968/71‑1 (MQ=255)
                                                                     |                                                                      
GGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCA  >  minE/1730904‑1731043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: