| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,730,976 | C→T | 5.2% | V87V (GTG→GTA) | yfhB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,730,976 | 0 | C | T | 5.2% | 361.1 / 6.1 | 115 | V87V (GTG→GTA) | yfhB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (45/64); new base T (2/4); total (47/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
AACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCA > minE/1730911‑1731043 | aaCAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGa < 1:195768/70‑1 (MQ=255) aCAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGaa < 1:396533/70‑1 (MQ=255) aCAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGa < 1:3354926/69‑1 (MQ=255) cAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCAACGTACGAAAt < 1:4559766/71‑1 (MQ=255) cAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCAACGTACGAAAt < 1:2632336/71‑1 (MQ=255) ggtaggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCg < 1:3968556/71‑1 (MQ=255) gtaggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCg < 1:1869840/70‑1 (MQ=255) aggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCACGAAACCAACGTACGAAAt < 1:1983355/66‑1 (MQ=255) aggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGT‑‑CGAAACCAGCGCACGAAATCGGCCTg > 1:1793219/1‑71 (MQ=255) gtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACg < 1:3938748/60‑1 (MQ=255) gtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACg < 1:1758789/60‑1 (MQ=255) gtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGaa < 1:1393675/62‑1 (MQ=255) gtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGc < 1:4449003/68‑1 (MQ=255) tggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:4404904/71‑1 (MQ=255) tggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:3823864/71‑1 (MQ=255) gtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCa < 1:608872/70‑1 (MQ=255) ttAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAAc > 1:2308828/1‑71 (MQ=255) ttAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAAc < 1:4476514/71‑1 (MQ=255) aaTCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTG‑AACGTc > 1:4570563/1‑71 (MQ=255) aTCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTc > 1:2230014/1‑71 (MQ=255) tCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCt < 1:1487056/62‑1 (MQ=255) tCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:369365/64‑1 (MQ=255) tCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:3371368/64‑1 (MQ=255) cGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAAc > 1:579856/1‑66 (MQ=255) gCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAAt > 1:1592637/1‑54 (MQ=255) gCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTg > 1:2076465/1‑70 (MQ=255) ccTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCa > 1:2751103/1‑45 (MQ=255) cTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaa < 1:3986754/69‑1 (MQ=255) gAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGt > 1:3702402/1‑71 (MQ=255) aaCCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACg < 1:4089535/69‑1 (MQ=255) cAGCGGATAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCtt > 1:525245/1‑71 (MQ=255) cAGCGGAAAGGTGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCtt > 1:3747770/1‑71 (MQ=255) cAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCtt > 1:4205628/1‑71 (MQ=255) gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAAt < 1:458470/42‑1 (MQ=255) gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGc > 1:3109011/1‑67 (MQ=255) gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCg < 1:1307877/71‑1 (MQ=255) gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTG‑AACGTCTGTAAACGTGCTTCGc < 1:52963/71‑1 (MQ=255) ggAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt < 1:2648884/71‑1 (MQ=255) ggAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt < 1:112759/71‑1 (MQ=255) gAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCaa > 1:4394517/1‑49 (MQ=255) gAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtg < 1:457597/71‑1 (MQ=255) gAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtg < 1:2563332/71‑1 (MQ=255) gAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtg > 1:597706/1‑71 (MQ=255) aaaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGTCCTGCAACGTCTGTa < 1:2282389/56‑1 (MQ=255) aaaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTa < 1:4199133/56‑1 (MQ=255) aaaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtgt < 1:2522904/71‑1 (MQ=255) aaaGGCGGTAACATTGTCACGAAACCAACGTACGAAATCGGCCTGCAACGCCTGCAAAc > 1:1365486/1‑59 (MQ=255) aaaGGCGGTAACATTGTCACGAAACCAACGTACGAAATCGGCCTGCAACGCCTGCAAAc > 1:1662872/1‑59 (MQ=255) aaGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAAc > 1:4430056/1‑58 (MQ=255) aGGCGTTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGa > 1:2169385/1‑71 (MQ=255) aGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaaa < 1:2181783/56‑1 (MQ=255) gCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGAcc < 1:3355850/71‑1 (MQ=255) gCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGAcc < 1:3361439/71‑1 (MQ=255) ggTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaa < 1:2097716/71‑1 (MQ=255) ggTAACATTGTCGCGAAACCAGCCCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaa < 1:419404/71‑1 (MQ=255) gTAACATTGTCGCGAACCCAGCGCACGAAATCGGCCTGCAACGTCTGtt < 1:1230132/49‑2 (MQ=255) gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGt < 1:3420914/44‑1 (MQ=255) gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTctct < 1:1348043/62‑1 (MQ=255) gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt < 1:2310990/62‑1 (MQ=255) gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt < 1:2316440/62‑1 (MQ=255) gTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaa < 1:226426/70‑1 (MQ=255) gTAACATTGTCGCGAAACCAGCGCACAAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaa < 1:2370295/71‑1 (MQ=255) tAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa < 1:2142580/71‑1 (MQ=255) tAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa < 1:648364/71‑1 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGc < 1:643913/37‑1 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGt > 1:4223662/1‑42 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaaa > 1:3270307/1‑49 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaa > 1:4087585/1‑48 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAg > 1:771745/1‑71 (MQ=255) aaCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAg > 1:2895023/1‑71 (MQ=255) aCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAg < 1:1433584/70‑1 (MQ=255) aCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGt > 1:2285043/1‑71 (MQ=255) aTTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaa > 1:4403098/1‑65 (MQ=255) ttGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTa < 1:3870381/43‑1 (MQ=255) ttGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCa > 1:2740419/1‑71 (MQ=255) gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTaa < 1:25514/42‑1 (MQ=255) gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAAc > 1:2213488/1‑44 (MQ=255) gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCtg > 1:2040178/1‑55 (MQ=255) gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCAcc > 1:3162841/1‑71 (MQ=255) gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCAcc > 1:635963/1‑71 (MQ=255) gTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCAcc > 1:4170451/1‑71 (MQ=255) tCGCGAAACCATCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCAccc < 1:1322238/71‑1 (MQ=255) gcgAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGAcc > 1:636868/1‑57 (MQ=255) gcgAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa > 1:4259676/1‑61 (MQ=255) gcgAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa > 1:1423753/1‑61 (MQ=255) cgAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCca < 1:2107634/71‑1 (MQ=255) gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaa < 1:4503128/58‑1 (MQ=255) gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCca > 1:1940133/1‑70 (MQ=255) gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCca > 1:172215/1‑70 (MQ=255) gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCca > 1:1443659/1‑70 (MQ=255) gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCca > 1:3862642/1‑70 (MQ=255) gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGT‑ACCAAAAGTg < 1:159598/61‑1 (MQ=255) gAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGGGACCAAAAGTGCACCCCca > 1:177574/1‑70 (MQ=255) aaCCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACg < 1:343484/38‑1 (MQ=255) aaCCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCca > 1:2073484/1‑68 (MQ=255) aaCCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAg < 1:514049/71‑1 (MQ=255) aaCCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAg < 1:4480378/71‑1 (MQ=255) ccAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCaaaa > 1:570675/1‑55 (MQ=255) cAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAg < 1:774149/71‑1 (MQ=255) gcgcACGAAATCGTCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCa < 1:3131968/71‑1 (MQ=255) | AACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCA > minE/1730911‑1731043 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |