| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,936,265 | G→A | 5.2% | V370V (GTC→GTT) | recJ ← | ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,936,265 | 0 | G | A | 5.2% | 304.3 / 4.2 | 95 | V370V (GTC→GTT) | recJ | ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Reads supporting (aligned to +/- strand): ref base G (41/49); new base A (2/3); total (43/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
TCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCACGGCTGCGCTCCA > minE/1936197‑1936335 | tCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGa > 1:2384058/1‑70 (MQ=255)tCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGAc < 1:84228/71‑1 (MQ=255) aCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACaa > 1:1560042/1‑71 (MQ=255) aCCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACaa > 1:2802002/1‑71 (MQ=255) aCCTGCTGGCGAAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACaa > 1:3696156/1‑71 (MQ=255) ccTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAAc < 1:1249402/71‑1 (MQ=255) tgctgGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACaa > 1:777631/1‑68 (MQ=255) tgctgGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGc > 1:72816/1‑71 (MQ=255) gctgGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGcc > 1:3404605/1‑71 (MQ=255) ctgGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGcc < 1:3760568/70‑1 (MQ=255) gcgcAAACGCGATAACCGGACGGTGAAAACGCTTTTTGATGCGCGAAGCCAGAATACCGACAACGCCCt < 1:3030602/69‑1 (MQ=255) gcgcAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCt < 1:1348590/69‑1 (MQ=255) gcgcAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCt < 1:2258066/69‑1 (MQ=255) gcgcAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCt < 1:4677479/69‑1 (MQ=255) gcAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATg > 1:469383/1‑71 (MQ=255) gcAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATg < 1:1862926/71‑1 (MQ=255) cAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGc < 1:1012487/71‑1 (MQ=255) cAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGc < 1:2173753/71‑1 (MQ=255) cAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGc > 1:1566342/1‑71 (MQ=255) aaaGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGcc < 1:1006588/71‑1 (MQ=255) aaaGGCGATAACCGGACGGTGAAAACGCTCTTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGcc < 1:1607817/71‑1 (MQ=255) aaaCGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGc > 1:3413475/1‑70 (MQ=255) aaCGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGc < 1:4397949/69‑1 (MQ=255) aCGCGATAACCGGACGGTGAAACCGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCAt > 1:1970958/1‑71 (MQ=255) aCGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCa > 1:3316508/1‑70 (MQ=255) cgcgATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCAtt < 1:1777652/71‑1 (MQ=255) gcgATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTc > 1:4390214/1‑71 (MQ=255) gcgATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTc < 1:413474/71‑1 (MQ=255) aTAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCggg < 1:4064388/71‑1 (MQ=255) tAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCgggg < 1:2518901/71‑1 (MQ=255) aCCTGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGt < 1:1713670/70‑1 (MQ=255) ccGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGa < 1:2347892/71‑1 (MQ=255) ggACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATa < 1:3440101/71‑1 (MQ=255) ggACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATa < 1:1823114/71‑1 (MQ=255) gACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATAc < 1:3181319/71‑1 (MQ=255) aCGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGa > 1:2615641/1‑52 (MQ=255) ggTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACAtt < 1:4301011/71‑1 (MQ=255) gTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCt < 1:2707206/47‑1 (MQ=255) gTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCt < 1:3243164/47‑1 (MQ=255) tGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGa < 1:3790536/63‑1 (MQ=255) tGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGAt < 1:729779/64‑1 (MQ=255) tGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGc < 1:1654039/71‑1 (MQ=255) aaaaCGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCg < 1:4006942/55‑1 (MQ=255) aaaaCGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACAtt > 1:1114265/1‑67 (MQ=255) aaaaCGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGcc < 1:1583279/70‑1 (MQ=255) aaaaCGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGc > 1:2776513/1‑69 (MQ=255) aaaCGCTCTTTGATGCGCGAAGCCAGAATACCGAc < 1:3228804/35‑1 (MQ=255) aaCGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGc < 1:1157516/47‑1 (MQ=255) cGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGa > 1:2667700/1‑57 (MQ=255) cGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGccc > 1:210671/1‑71 (MQ=255) cGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGccc > 1:1973284/1‑71 (MQ=255) gCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACAtt < 1:3781513/62‑1 (MQ=255) gCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCccg > 1:1335089/1‑71 (MQ=255) ctctTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCccgc > 1:492313/1‑71 (MQ=255) ctctTTGATACGCGATGCCAGAATGCCAACAACGCCCTGATGCCATTCAGGGTGATACATTGCCAGcccac < 1:2228018/71‑3 (MQ=255) tctTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACa > 1:1211429/1‑58 (MQ=255) tctTTGATGCGCGAAGCCAGAATACCGACAACGCACTGATGCCATTCGGGGTGATACa > 1:2543836/1‑58 (MQ=255) tttGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCAtt > 1:368876/1‑44 (MQ=255) tttGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCAt < 1:1035930/43‑1 (MQ=255) tttGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGa > 1:4469350/1‑52 (MQ=255) tttGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTg < 1:4190407/59‑1 (MQ=255) tttGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCgg > 1:3509719/1‑71 (MQ=255) tttGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCgg > 1:721625/1‑71 (MQ=255) tttGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCgg > 1:3693228/1‑71 (MQ=255) tttGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCgg > 1:2601104/1‑71 (MQ=255) tttGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCAAGCCCGCCgg > 1:2943506/1‑71 (MQ=255) tttGAAGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCgg > 1:2784141/1‑71 (MQ=255) tGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATa > 1:1554590/1‑52 (MQ=255) tGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACa > 1:1945234/1‑54 (MQ=255) tGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACa > 1:3041472/1‑54 (MQ=255) tGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACa > 1:4047699/1‑54 (MQ=255) tGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCccgccg < 1:210214/68‑1 (MQ=255) tGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCgg < 1:2067016/69‑1 (MQ=255) tGATGCGCGAAGCCAGAATACCGAAAACGCCCTGATGCCATTCGGGGTGATACATTGCCCGc > 1:2547244/1‑62 (MQ=255) aTGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAg < 1:1054943/71‑1 (MQ=255) cgcgAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACa < 1:4402823/49‑1 (MQ=255) cgcgAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCg < 1:169101/70‑1 (MQ=255) cgAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGt < 1:4341002/41‑1 (MQ=255) cgAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCgtgt < 1:2067757/71‑1 (MQ=255) cgAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCgtgt > 1:1758279/1‑71 (MQ=255) gAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATT‑CCAGCCCGCCGGGTAGCGTGTCa < 1:2544932/71‑1 (MQ=255) aaGCCAGAATACCGACAACGCCCTGATGCCATTCGTGGTGATACa > 1:2706926/1‑45 (MQ=255) aaGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCa > 1:2417378/1‑71 (MQ=255) aGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCAc < 1:1913254/71‑1 (MQ=255) ccAGAATACCGACAACGCCCTGATGCCATTCgggg < 1:439656/35‑1 (MQ=255) ccAGAATACCGACAACGCCCTGATGCCATTCGGGGt < 1:725565/36‑1 (MQ=255) ccAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACAtt < 1:3037881/44‑1 (MQ=255) gAATGCCAACACCGCCCTGATGCCATTCAGGGTGATACATTGCCAGccca > 1:2093469/1‑49 (MQ=255) gAATGCCAACAACGCCCTGATGCCATTCAGGGTGATACATTGCCAGCCCACCTGGCAGCGTGTCGCGGCta > 1:2472693/1‑70 (MQ=255) aTACCGACAACGCCCTGATGCCATTCGGGGTGATa > 1:2403989/1‑35 (MQ=255) tACCGACAACGCCCTGATGCCATTCGGGGTGATACAt < 1:1428526/37‑1 (MQ=255) tACCGACAACGCCCTGATGCCATTCGGGGTGATACAt < 1:2123838/37‑1 (MQ=255) aCCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCAc < 1:1981876/62‑1 (MQ=255) ccGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCACGGc > 1:1502947/1‑64 (MQ=255) cGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCACGGCTGCGCTcc < 1:784016/71‑1 (MQ=255) gACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCACGGCTGCGCTCCa < 1:3414912/71‑1 (MQ=255) | TCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAAACGCTCTTTGATGCGCGAAGCCAGAATACCGACAACGCCCTGATGCCATTCGGGGTGATACATTGCCAGCCCGCCGGGTAGCGTGTCACGGCTGCGCTCCA > minE/1936197‑1936335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |