| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,941,030 | T→G | 5.3% | T150T (ACT→ACG) | ygfZ → | predicted folate‑dependent regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,941,030 | 0 | T | G | 5.3% | 188.2 / 5.5 | 75 | T150T (ACT→ACG) | ygfZ | predicted folate‑dependent regulatory protein |
| Reads supporting (aligned to +/- strand): ref base T (43/28); new base G (2/2); total (45/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGC > minE/1940961‑1941097 | cgcgcTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACtt < 1:3500647/71‑1 (MQ=255) gcgcTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACttt > 1:1047728/1‑71 (MQ=255) cgcTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg > 1:1285810/1‑71 (MQ=255) cgcTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg > 1:2609530/1‑71 (MQ=255) gcTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGc < 1:703601/71‑1 (MQ=255) cTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGc < 1:2045905/70‑1 (MQ=255) gCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATg > 1:800316/1‑71 (MQ=255) cAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATgg > 1:3013991/1‑71 (MQ=255) ctctTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTg > 1:2094857/1‑70 (MQ=255) tctTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGaa > 1:1112120/1‑71 (MQ=255) tctTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGaa > 1:1400866/1‑71 (MQ=255) tttAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGaa > 1:4214258/1‑69 (MQ=255) tAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACt > 1:520176/1‑52 (MQ=255) tAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACt > 1:1139657/1‑52 (MQ=255) tAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGaa > 1:1821679/1‑67 (MQ=255) tAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAacac < 1:1519541/70‑1 (MQ=255) tAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACAcc > 1:1538093/1‑71 (MQ=255) aGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGaa > 1:2608461/1‑66 (MQ=255) aGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACAcc < 1:542491/70‑1 (MQ=255) cGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCgg < 1:1699639/71‑1 (MQ=255) gAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAG‑‑GCGACCACTTTGCTATGGTTTGAACACCCGGCAg < 1:119068/71‑1 (MQ=255) cTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTa > 1:2482274/1‑51 (MQ=255) cTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGaa > 1:1403981/1‑60 (MQ=255) tGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg < 1:699981/47‑1 (MQ=255) tGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATgg < 1:4358726/53‑1 (MQ=255) ccTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGGGACCACTTTGCTATGGtt > 1:1332520/1‑53 (MQ=255) ccTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACtt > 1:2736844/1‑43 (MQ=255) ccTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACt > 1:818846/1‑42 (MQ=255) ccTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGaa > 1:3144951/1‑57 (MQ=255) ccTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACg > 1:1543446/1‑71 (MQ=255) ccTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACg > 1:438279/1‑71 (MQ=255) cTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGt < 1:1768656/71‑1 (MQ=255) tCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAAc > 1:742811/1‑67 (MQ=255) cGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGtt < 1:2596298/69‑1 (MQ=255) cGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTc > 1:4278848/1‑71 (MQ=255) cGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTc > 1:1736268/1‑71 (MQ=255) gaaagaaaAACAGGTAGTCAAAGAAGGCGCGACCACtt > 1:2957587/1‑38 (MQ=255) gaaagaaaAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTc < 1:4091661/70‑1 (MQ=255) agagaaaAACAGGTAGTCAAAGAAGGCGCGACCACGTTGCTATGGTTTGAACACCCGGCAGAACGt < 1:4559330/64‑1 (MQ=255) agagaaaAACAGGTAGTCAAAGAAGGCGCGACCACGTTGCTATGGTTTGAACACCCGGCAGAACGt < 1:4220025/64‑1 (MQ=255) aaagaaaAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAg < 1:4110454/61‑1 (MQ=255) aaagaaaAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCt > 1:3379651/1‑71 (MQ=255) aagaaaAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCt > 1:4061097/1‑70 (MQ=255) gaaaAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAAc < 1:3641200/61‑1 (MQ=255) gaaaAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAAc < 1:4345917/61‑1 (MQ=255) aGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGt < 1:2218723/36‑1 (MQ=255) aGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAAcc < 1:2366322/71‑1 (MQ=255) gTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATg < 1:84072/70‑1 (MQ=255) tCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAg < 1:2585863/45‑1 (MQ=255) tCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGtt < 1:2398680/51‑1 (MQ=255) cAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTg < 1:635469/55‑1 (MQ=255) cAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAg > 1:4444878/1‑71 (MQ=255) cAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAg > 1:1300192/1‑71 (MQ=255) aaaGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGa > 1:3335001/1‑44 (MQ=255) aaaGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAAc > 1:3234423/1‑62 (MQ=255) aaaGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGc < 1:1750528/71‑1 (MQ=255) aaaGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGc > 1:4205584/1‑71 (MQ=255) aagaagGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGa < 1:2716001/67‑1 (MQ=255) agaagGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTa > 1:2615260/1‑71 (MQ=255) agaagGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTa > 1:2025364/1‑71 (MQ=255) gaagGCGCGACCACTTTGCTATGGTTTGAACACCCGGCa > 1:1167703/1‑39 (MQ=255) gaagGCGCGACCACTTTGCTATGGTTTGAACACCCGGCa > 1:4336908/1‑39 (MQ=255) gaagGCGCGACCACTTTGCTATGGTTTGAACACCCGGCa > 1:4068146/1‑39 (MQ=255) aagGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTaa > 1:2177649/1‑57 (MQ=255) agGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTg > 1:1389178/1‑71 (MQ=255) agGCGCGACCACGTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTaa > 1:666586/1‑56 (MQ=255) agGCGCGACCACGTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTg > 1:4352275/1‑71 (MQ=255) ggCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGc > 1:1046923/1‑71 (MQ=255) gcgcGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGt < 1:2712953/52‑1 (MQ=255) cgcgACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCt > 1:3458702/1‑70 (MQ=255) cgcgACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCt > 1:4695368/1‑70 (MQ=255) cgcgACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTa < 1:602725/71‑1 (MQ=255) cgcgACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTa < 1:984388/71‑1 (MQ=255) cgcgACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTa < 1:1556483/71‑1 (MQ=255) gcgACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAg > 1:484724/1‑61 (MQ=255) cgACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCt < 1:3472217/62‑1 (MQ=255) cgACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCt < 1:1520268/62‑1 (MQ=255) aCCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTAc > 1:3822081/1‑62 (MQ=255) ccACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATg > 1:1836600/1‑71 (MQ=255) cACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGc > 1:4325760/1‑71 (MQ=255) cACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGc > 1:3786701/1‑71 (MQ=255) cACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGc > 1:1662558/1‑71 (MQ=255) tttGCTATGGTTTGAACACCCGGCAGAACGTTTCCTg < 1:3537573/37‑1 (MQ=255) tttGCTATGGTTTGAACACCCGGCAGAACGTTTCCTg < 1:3137477/37‑1 (MQ=255) tttGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCt < 1:4677120/61‑1 (MQ=255) | CGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTTTGAACACCCGGCAGAACGTTTCCTGATCGTAACCGATGAAGCTACTGCTAATATGC > minE/1940961‑1941097 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |