Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,981,950	G→A	5.4%	P42L (CCG→CTG)	yggR ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,981,950	0	G	A	5.4%	231.1 / 2.3	74	P42L (CCG→CTG)	yggR	predicted transporter
Reads supporting (aligned to +/- strand):  ref base G (38/32);  new base A (2/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

GACCATTCTCCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGG  >  minE/1981882‑1982018                                                                     |                                                                     gACCATTCTCCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGc                                                                    >  1:3369145/1‑71 (MQ=255) gACCATTCTCCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGc                                                                    >  1:3514349/1‑71 (MQ=255) gACCATTCTCCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGc                                                                    >  1:1979914/1‑71 (MQ=255)  aCCATTCTCCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCg                                                                      >  1:1100654/1‑68 (MQ=255)   ccATTCTCCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGt                                                                  <  1:2159460/71‑1 (MQ=255)     aTTCTCCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGt                                                                  <  1:1673054/69‑1 (MQ=255)       tctcCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATc                                                               >  1:1381229/1‑70 (MQ=255)        ctcCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCa                                                              >  1:2491295/1‑70 (MQ=255)        ctcCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCa                                                              >  1:2026589/1‑70 (MQ=255)        ctcCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCa                                                              >  1:3596703/1‑70 (MQ=255)           cAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCTACGTCCGGCGTATCaaa                                                            <  1:1590946/69‑1 (MQ=255)           cAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCaaa                                                            <  1:1518193/69‑1 (MQ=255)             gCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGgc                                                        <  1:1428433/71‑1 (MQ=255)              cAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGgcg                                                       >  1:3443396/1‑71 (MQ=255)              cAATATTGTCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGgcg                                                       >  1:1494584/1‑71 (MQ=255)               aaTATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCaaa                                                            >  1:4563825/1‑65 (MQ=255)               aaTATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCaaa                                                            >  1:2075273/1‑65 (MQ=255)               aaTATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGgcgc                                                      <  1:3978872/71‑1 (MQ=255)                atatTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCaaa                                                            >  1:4357782/1‑64 (MQ=255)                atatTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCa                                                     <  1:2845524/71‑1 (MQ=255)                  atTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGt                                                                  >  1:1235749/1‑56 (MQ=255)                  atTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCaa                                                             <  1:2647128/61‑1 (MQ=255)                   ttGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCa                                                              <  1:3529424/59‑1 (MQ=255)                   ttGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCt                                                  >  1:4354587/1‑71 (MQ=255)                     gCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTAt                                                                >  1:1239848/1‑55 (MQ=255)                     gCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTc                                                >  1:564475/1‑71 (MQ=255)                        cGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCa                                              <  1:934249/70‑1 (MQ=255)                        cGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCAt                                             <  1:757352/71‑1 (MQ=255)                        cGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCAt                                             <  1:4361197/71‑1 (MQ=255)                        cGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCAt                                             <  1:3338813/71‑1 (MQ=255)                         gCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCAtt                                            >  1:587275/1‑71 (MQ=255)                             aTCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGgcg                                                       <  1:3126993/56‑1 (MQ=255)                             aTCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGc                                        >  1:1352948/1‑71 (MQ=255)                              tCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGcc                                       >  1:4269140/1‑71 (MQ=255)                               cGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCaaa                                                            >  1:371496/1‑49 (MQ=255)                               cGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCcg                                      >  1:4217663/1‑71 (MQ=255)                                gTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCAt                                             <  1:292636/63‑1 (MQ=255)                                gTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCAt                                             <  1:3361978/63‑1 (MQ=255)                                gTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCAt                                             <  1:3356429/63‑1 (MQ=255)                                gTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCcgc                                     >  1:1098831/1‑71 (MQ=255)                                 tCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTg                                         <  1:3325917/66‑1 (MQ=255)                                 tCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTg                                         <  1:3275344/66‑1 (MQ=255)                                 tCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCcgcg                                    <  1:2687496/71‑1 (MQ=255)                                  cATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCcgcg                                    >  1:758999/1‑70 (MQ=255)                                   aTCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTCCCGCGa                                   <  1:3195477/70‑1 (MQ=255)                                   aTCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGGCGCAGCTTCCATTCGCCCGCGa                                   <  1:4404115/70‑1 (MQ=255)                                   aTCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCAGCGCATCAAACGGCGCAGCTTCCATTCGCCCGCGa                                   <  1:2489930/70‑1 (MQ=255)                                    tCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCAtt                                            >  1:2873788/1‑60 (MQ=255)                                     ccAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTc                                           >  1:2494560/1‑60 (MQ=255)                                           aCTCCCGCAGTAGCTGTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                          >  1:931132/1‑71 (MQ=255)                                           aCTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                          <  1:3624108/71‑1 (MQ=255)                                               ccGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCa                                              <  1:3438087/47‑1 (MQ=255)                                               ccGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGt                       <  1:2983139/70‑1 (MQ=255)                                               ccGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTg                      >  1:2846382/1‑71 (MQ=255)                                               ccGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTg                      >  1:1699024/1‑71 (MQ=255)                                               ccGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTg                      >  1:2956170/1‑71 (MQ=255)                                               ccGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTg                      >  1:685074/1‑71 (MQ=255)                                                 gCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGa                                   >  1:1446041/1‑56 (MQ=255)                                                     tAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTc                                                <  1:4290717/39‑1 (MQ=255)                                                     tAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCAt                          <  1:2335113/61‑1 (MQ=255)                                                     tAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGc                 <  1:3053385/70‑1 (MQ=255)                                                      aGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGc                 >  1:3313562/1‑69 (MQ=255)                                                       gCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCt                                          <  1:792687/43‑1 (MQ=255)                                                         tctTCGACGTCCGGCGTATCAAACGGCGCAGCTTCAAt                                             <  1:1875154/38‑1 (MQ=255)                                                          ctTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGgcg           >  1:2500709/1‑71 (MQ=255)                                                           ttCGACGTCCGGCGTATCAAACGGCGCAGCTTCCa                                              >  1:1233171/1‑35 (MQ=255)                                                           ttCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGgcgc          >  1:4548554/1‑71 (MQ=255)                                                            tCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCt                                          <  1:4419259/38‑1 (MQ=255)                                                            tCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTg                      >  1:618770/1‑58 (MQ=255)                                                             cGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGgcg           <  1:3539393/68‑1 (MQ=255)                                                             cGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTg        >  1:4329289/1‑71 (MQ=255)                                                              gACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTg        >  1:514309/1‑70 (MQ=255)                                                              gACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTg        >  1:3888515/1‑70 (MQ=255)                                                                 gTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGcac     <  1:1495239/70‑1 (MQ=255)                                                                   ccGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAgg  >  1:3530728/1‑71 (MQ=255)                                                                     ggCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAAt                                 <  1:264219/38‑1 (MQ=255)                                                                     |                                                                     GACCATTCTCCAGCAATATTGCCCGCTGATCGTCATCCAGCCACTCCCGCAGTAGCTCTTCGACGTCCGGCGTATCAAACGGCGCAGCTTCCATTCTGCCGCGAATGCGCCATCGTGCGGGCCAGGCGCTGCACAGG  >  minE/1981882‑1982018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑