| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,028,050 | G→A | 5.2% | I228I (ATC→ATT) | parC ← | DNA topoisomerase IV, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,028,050 | 0 | G | A | 5.2% | 305.5 / 4.1 | 96 | I228I (ATC→ATT) | parC | DNA topoisomerase IV, subunit A |
| Reads supporting (aligned to +/- strand): ref base G (42/49); new base A (2/3); total (44/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
CGCCATCTTCTTTCTTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTGCACGATATCCAGCAGCT > minE/2027983‑2028120 | cgcCATCTTCTTTCTTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGAtc > 1:3652996/1‑71 (MQ=255) gcaaTCTTCTTTCTTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGAtct < 1:3059955/68‑1 (MQ=255) gcCATCTTCTTTCTTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCTTAGATTTTACGGAtct < 1:1817566/71‑1 (MQ=255) gcCATCTTCTTTCTTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGAtct < 1:765495/71‑1 (MQ=255) ccATCTTCTTTCTTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACgg < 1:2630985/66‑1 (MQ=255) ccATCTTCTTTCTTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGAtctc > 1:360961/1‑71 (MQ=255) ccATCTTCTTTCTTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGAtctc < 1:1802292/71‑1 (MQ=255) tcttctTTCTTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACgg < 1:4343179/63‑1 (MQ=255) tcttctTTCTTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGgc > 1:4133536/1‑71 (MQ=255) ttctttctTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGa > 1:1088265/1‑62 (MQ=255) ttctttctTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGAt > 1:3840424/1‑63 (MQ=255) ttctttctTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGgcgc > 1:809426/1‑71 (MQ=255) ttctttctTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGgcgc > 1:248189/1‑71 (MQ=255) ttctttctTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGgc > 1:400177/1‑69 (MQ=255) tctttcttCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGgcgc > 1:1484122/1‑70 (MQ=255) tcttcCACACCGCGCGCATACGCACTGAACCACTTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGa < 1:2702278/70‑1 (MQ=255) tcttcCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGcgcgcg > 1:4675969/1‑69 (MQ=255) tcttcCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGcgcgcg < 1:4411750/69‑1 (MQ=255) tcttcCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGcgcgcg < 1:2032721/69‑1 (MQ=255) cttcCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAg > 1:1272233/1‑71 (MQ=255) ccACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTg < 1:1797134/70‑1 (MQ=255) cacaCCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAg < 1:1482304/67‑1 (MQ=255) acacCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGAATCTCGGCGCGCGAAGTGAt > 1:4089924/1‑70 (MQ=255) acacCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGAATCTCGGCGCGCGAAGTGAt > 1:4075234/1‑70 (MQ=255) cacCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGAtctct > 1:3015152/1‑54 (MQ=255) cacCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATaa > 1:756882/1‑71 (MQ=255) cgcgcgCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGAt < 1:1284228/48‑1 (MQ=255) cgcgcgCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAAttt < 1:1911660/71‑1 (MQ=255) cgcgcgCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAAtt < 1:3042746/70‑1 (MQ=255) cgcgcgCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAAtt > 1:376701/1‑70 (MQ=255) cgcgcATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGAtct > 1:2754368/1‑48 (MQ=255) gcATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGAATCTCGGCGCGCGAAGTGATAATTTCCGCt < 1:4261676/71‑1 (MQ=255) aTACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCtt > 1:2148494/1‑70 (MQ=255) aTACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGAATCTCGGCGCGCGAAGt < 1:218165/56‑1 (MQ=255) tACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCa < 1:4343049/71‑1 (MQ=255) tACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCa < 1:4529776/71‑1 (MQ=255) tACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGAATCTCGGCGCGCGAAGt < 1:3871986/55‑1 (MQ=255) cGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAg < 1:4189866/70‑1 (MQ=255) cGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAg > 1:3781655/1‑70 (MQ=255) cGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGt > 1:1126606/1‑71 (MQ=255) cGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGt > 1:2437685/1‑71 (MQ=255) cACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCg > 1:4296029/1‑71 (MQ=255) aCTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCgg < 1:1353586/71‑1 (MQ=255) aCTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCgg < 1:636099/71‑1 (MQ=255) aCTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCg > 1:901336/1‑70 (MQ=255) aCTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCCCGAAGTGATAATTTCCGCTTCAGTCgg < 1:3642776/71‑1 (MQ=255) cTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGcgcgcg < 1:1896858/45‑1 (MQ=255) cTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGcgcgcg < 1:1705908/45‑1 (MQ=255) cTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAAtt < 1:2482555/56‑1 (MQ=255) cTGAACCACGTCCGTTCTCGTAGATTTCACGGAtct < 1:3663786/36‑1 (MQ=255) cTGAACCACGTCCGTTCACGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGa < 1:2017788/71‑1 (MQ=255) tGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAAttt > 1:3903760/1‑56 (MQ=255) tGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCa < 1:1781974/64‑1 (MQ=255) tGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGa > 1:2301736/1‑70 (MQ=255) gAACCACGTCCGTTCTCGTAGATTTTACGGATCTGGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGAt > 1:2758607/1‑70 (MQ=255) gAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATaa > 1:3513738/1‑52 (MQ=255) gAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGAt > 1:22978/1‑70 (MQ=255) gAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGAt > 1:3647858/1‑70 (MQ=255) aaCCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGAt < 1:746565/69‑1 (MQ=255) aaCCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGAt > 1:2422578/1‑69 (MQ=255) aaCCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATaa < 1:2075690/71‑1 (MQ=255) ccACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCTCGAAGTGAt < 1:3046334/47‑1 (MQ=255) ccACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTc > 1:3503804/1‑63 (MQ=255) cACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTct < 1:1623125/63‑2 (MQ=255) aCGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGaa > 1:1620070/1‑40 (MQ=255) aCGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCg > 1:2586279/1‑71 (MQ=255) cGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCg > 1:1714799/1‑70 (MQ=255) gTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGa < 1:4384547/37‑1 (MQ=255) gTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTg < 1:2755522/41‑1 (MQ=255) tCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGAt < 1:3703570/42‑1 (MQ=255) tCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGcc < 1:1580229/71‑1 (MQ=255) tCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGcc < 1:1889194/71‑1 (MQ=255) ccGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTACGCtt > 1:430644/1‑52 (MQ=255) cGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTc < 1:2355970/46‑1 (MQ=255) cGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGcccc > 1:4673053/1‑71 (MQ=255) cGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGccc > 1:1361984/1‑70 (MQ=255) gTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCtt < 1:3342892/50‑1 (MQ=255) ttCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCt < 1:1913873/48‑1 (MQ=255) ttCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGa < 1:2951894/57‑1 (MQ=255) ttCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTg < 1:1129412/71‑1 (MQ=255) ttCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTg < 1:3391220/71‑1 (MQ=255) tctcGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAAtt < 1:3247817/41‑1 (MQ=255) tctcGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTg > 1:918468/1‑70 (MQ=255) ctcGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATc > 1:2842316/1‑60 (MQ=255) ctcGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATc > 1:1375577/1‑60 (MQ=255) gTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTGCACGa < 1:942587/71‑1 (MQ=255) gTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTGCACGa > 1:225297/1‑71 (MQ=255) tAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCa < 1:4126314/45‑1 (MQ=255) tAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCa < 1:83642/45‑1 (MQ=255) tAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCa < 1:3065378/45‑1 (MQ=255) tAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGcg < 1:491108/61‑2 (MQ=255) gATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGt > 1:295206/1‑45 (MQ=255) tttACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATcc < 1:4456133/52‑1 (MQ=255) tttACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTGCACGATATc > 1:2344916/1‑69 (MQ=255) tACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTGCACGATATCcag > 1:1292703/1‑70 (MQ=255) aCGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTGCACGATATCcagc < 1:4655860/70‑1 (MQ=255) aCGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTGCACGATATCcagc < 1:43382/70‑1 (MQ=255) aCGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTGCACGATATCcagc < 1:4018429/70‑1 (MQ=255) ggATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTc > 1:3882894/1‑38 (MQ=255) gATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTGCACGATATCCAGCAGCt > 1:2437513/1‑71 (MQ=255) | CGCCATCTTCTTTCTTCCACACCGCGCGCATACGCACTGAACCACGTCCGTTCTCGTAGATTTTACGGATCTCGGCGCGCGAAGTGATAATTTCCGCTTCAGTCGGATAATCCGGCCCCTGCACGATATCCAGCAGCT > minE/2027983‑2028120 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |