| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,226,993 | G→A | 5.8% | L22L (CTG→CTA) | yhdY → | predicted amino‑acid transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,226,993 | 0 | G | A | 5.8% | 213.5 / 2.4 | 69 | L22L (CTG→CTA) | yhdY | predicted amino‑acid transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (33/32); new base A (2/2); total (35/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
AGGACATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGC > minE/2226924‑2227060 | aGGACATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCctgc > 1:2140974/1‑71 (MQ=255)aGGACATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCctg > 1:550492/1‑70 (MQ=255) aaCTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTAt > 1:3403381/1‑71 (MQ=255) aCTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTAtt > 1:1133014/1‑71 (MQ=255) aCTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATATGTTCTCCAGCTGGAGCAATAGCCTGCTGACTAtt < 1:252504/71‑1 (MQ=255) aCTTAAGACGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTAtt > 1:84233/1‑71 (MQ=255) aaGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCt > 1:4093639/1‑71 (MQ=255) aaGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCt > 1:1834933/1‑71 (MQ=255) aGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCt < 1:1022956/70‑1 (MQ=255) ccGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCa < 1:4558359/71‑1 (MQ=255) cgcgcgATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGc > 1:1713963/1‑69 (MQ=255) cgcgcgATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCat < 1:1367537/71‑1 (MQ=255) gcgATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACt < 1:2634916/57‑1 (MQ=255) gcgATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATg > 1:737229/1‑71 (MQ=255) cgATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATgg > 1:3308894/1‑71 (MQ=255) tGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCctgctg > 1:4222179/1‑50 (MQ=255) tGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGtt > 1:87167/1‑70 (MQ=255) ggTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGTTGACTa < 1:602557/53‑1 (MQ=255) ggTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCa > 1:287715/1‑62 (MQ=255) gctgtgGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGa > 1:567663/3‑71 (MQ=255) tgGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGa < 1:3371731/67‑1 (MQ=255) gggTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTAtt > 1:4186508/1‑50 (MQ=255) ggTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTggg < 1:2409310/71‑1 (MQ=255) ggTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTggg < 1:2993524/71‑1 (MQ=255) ggTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTggg < 1:289379/71‑1 (MQ=255) ggTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTggg < 1:4505528/71‑1 (MQ=255) ggTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTggg < 1:1218425/71‑1 (MQ=255) ggTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTgg > 1:2329580/1‑70 (MQ=255) ggTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTgg > 1:3450285/1‑70 (MQ=255) ggTGCGAAAAAATCTGTTCTCCAGCCGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTggg < 1:3160029/71‑1 (MQ=255) tGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGAtgt < 1:401344/66‑1 (MQ=255) gCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGGTGCata > 1:3038875/1‑55 (MQ=255) gCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAAc > 1:3860027/1‑71 (MQ=255) cGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACt < 1:4674784/71‑1 (MQ=255) gAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTg < 1:3889991/71‑1 (MQ=255) gAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTg < 1:961718/71‑1 (MQ=255) aaaaaaTCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTg < 1:2587297/70‑1 (MQ=255) aaaaaaTCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGa > 1:1731171/1‑71 (MQ=255) aaaaaaTCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTT‑ATGTggg < 1:401257/64‑1 (MQ=255) aaaaaTCTGTTCTCCAGCTGGAGCAATAGCctact > 1:1104691/1‑35 (MQ=255) aaaaaTCTGTTCTCCAGCTGGAGCAATAGCCTGCTGAc > 1:1875146/1‑38 (MQ=255) aaaaTCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGc < 1:1724359/47‑1 (MQ=255) aaaaTCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATc > 1:654310/1‑71 (MQ=255) cTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGCTCCCAc > 1:922906/1‑70 (MQ=255) tGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGa < 1:3021737/51‑1 (MQ=255) tGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCg > 1:177850/1‑71 (MQ=255) ttCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCg < 1:1967809/69‑1 (MQ=255) tcCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCt > 1:3503139/1‑71 (MQ=255) cAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGa > 1:2054257/1‑56 (MQ=255) cAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGGTg < 1:1082057/70‑1 (MQ=255) tGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTggg < 1:32138/46‑1 (MQ=255) ggAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTgg > 1:4496224/1‑71 (MQ=255) gAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTgg > 1:2464308/1‑70 (MQ=255) gAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTg < 1:3758459/69‑1 (MQ=255) gAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTg < 1:3131491/69‑1 (MQ=255) aGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGAtgt > 1:1894021/1‑40 (MQ=255) gCAATAGCCTGCTGACTATTGGCTGCATATGGTTGAt > 1:4050423/1‑37 (MQ=255) gCAATAGCCTACTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCg > 1:3591917/1‑56 (MQ=255) gCAATAGCCTACTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCa > 1:3388920/1‑71 (MQ=255) aaTAGCCTACTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTg < 1:1806962/57‑1 (MQ=255) aaTAGCCTACTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGa < 1:71722/61‑1 (MQ=255) aTAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGTAACTGATCCCACCGTTGCTGAAc > 1:4474547/1‑62 (MQ=255) aTAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGt > 1:1149118/1‑54 (MQ=255) aTAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGt < 1:743581/54‑1 (MQ=255) tAGCCTGCTGACTATTGGCTGCATATGGTTGATGTggg < 1:2847924/38‑1 (MQ=255) tAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCAtttt > 1:203853/1‑71 (MQ=255) aGCCTGCTGACTATTGGCTGCATATGGTTGAtgtg > 1:1434282/1‑35 (MQ=255) gCCTGCTGACTATTGGCTGCATATGGTTGATGTgg > 1:1809178/1‑35 (MQ=255) gCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGaa > 1:189925/1‑38 (MQ=255) gCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTg < 1:2235831/53‑1 (MQ=255) gCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCAttttt < 1:944179/70‑1 (MQ=255) ccTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGc < 1:527763/71‑1 (MQ=255) ccTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGc < 1:4321730/71‑1 (MQ=255) ctgctgACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGc < 1:2239869/70‑1 (MQ=255) tgctgACTATTGGCTGCATATGGTTGATGTGGGAACTGAt < 1:3387361/40‑1 (MQ=255) gctgACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTg < 1:4583243/67‑1 (MQ=255) | AGGACATAACTTAAGCCGCGCGATGGTGTGGGTGCGAAAAAATCTGTTCTCCAGCTGGAGCAATAGCCTGCTGACTATTGGCTGCATATGGTTGATGTGGGAACTGATCCCACCGTTGCTGAACTGGGCATTTTTGC > minE/2226924‑2227060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |