BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,359,876	C→T	36.9%	noncoding (1219/2905 nt)	rrlA ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,359,876	0	C	T	36.9%	181.6 / 160.9	162	noncoding (1219/2905 nt)	rrlA	23S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base C (54/41); new base T (33/26); total (93/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 168 total aligned reads displayed.

CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTGC  >  minE/2359815‑2359946

cccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                               <  1:3987377/71‑1 (MQ=9)

 ccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca                                                               <  1:564710/70‑1 (MQ=31)

 ccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcac                                                              <  1:1355553/71‑1 (MQ=255)

  cGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca                                                               >  1:2358443/1‑69 (MQ=31)

  cGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcac                                                              <  1:2005393/70‑1 (MQ=255)

   gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATAACTCACAGTACACCTTCACAg                                                            >  1:25703/1‑71 (MQ=11)

   gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAg                                                            >  1:3522401/1‑71 (MQ=11)

   gCTTAATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcagg                                                             >  1:608013/1‑68 (MQ=9)

       tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                                         >  1:929185/1‑70 (MQ=35)

       tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                                         >  1:4215648/1‑70 (MQ=255)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                                       >  1:17193/1‑70 (MQ=35)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                                       >  1:2470281/1‑70 (MQ=35)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTa                                                       <  1:2036201/70‑1 (MQ=255)

           gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                    <  1:2644620/71‑1 (MQ=34)

           gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                               <  1:4585194/60‑1 (MQ=11)

           gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTa                                                       >  1:2094362/1‑68 (MQ=255)

           gTTACTTATGCCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                    <  1:2962223/71‑1 (MQ=21)

             tACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGaa                                                  >  1:1269440/1‑71 (MQ=255)

              aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa                                                  <  1:783686/70‑1 (MQ=35)

              aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAAc                                                 >  1:74871/1‑71 (MQ=34)

              aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcaca                                                             >  1:1662393/1‑59 (MQ=11)

              aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAAc                                                 >  1:465139/1‑71 (MQ=255)

                 tATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCtt                                                        <  1:3488283/61‑1 (MQ=35)

                 tATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCtt                                                        <  1:3342173/61‑1 (MQ=35)

                  aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                               <  1:215768/69‑1 (MQ=35)

                  aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                             >  1:3762593/1‑71 (MQ=34)

                  aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                             >  1:2729010/1‑71 (MQ=255)

                   tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                            <  1:1973318/71‑1 (MQ=34)

                   tGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                             <  1:54783/70‑1 (MQ=255)

                     tCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcac                                                              <  1:3579529/51‑1 (MQ=11)

                     tCAGCACTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcac                                                              <  1:4392679/51‑1 (MQ=11)

                       aGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTa                                        >  1:3173258/1‑71 (MQ=34)

                         cATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                                         >  1:2879683/1‑52 (MQ=35)

                         cATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc                                           >  1:4582476/1‑66 (MQ=35)

                           ttCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcac                                                              >  1:122100/1‑45 (MQ=11)

                           ttCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg                                                    <  1:4136902/55‑1 (MQ=11)

                           ttCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg                                                    <  1:1260279/55‑1 (MQ=11)

                            tCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACg                                                >  1:2282957/1‑58 (MQ=11)

                            tCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACg                                                >  1:3054334/1‑58 (MQ=11)

                            tCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACg                                                >  1:2054159/1‑58 (MQ=11)

                            tCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCt                                         <  1:4017568/65‑1 (MQ=255)

                             cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                                      >  1:721464/1‑67 (MQ=35)

                             cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                                   >  1:427031/1‑70 (MQ=35)

                             cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                                   >  1:3613028/1‑70 (MQ=35)

                             cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                                   >  1:3880066/1‑70 (MQ=35)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGaa                                                  <  1:3773015/55‑1 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                                   >  1:274157/1‑70 (MQ=255)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                                   >  1:890322/1‑70 (MQ=255)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                                   >  1:702745/1‑70 (MQ=255)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                                   >  1:4319380/1‑70 (MQ=255)

                              gCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                                   >  1:2821204/1‑69 (MQ=35)

                              gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGaa                                                  >  1:1073557/1‑54 (MQ=11)

                              gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                                  <  1:4186127/70‑1 (MQ=255)

                              gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                                  >  1:3601662/1‑70 (MQ=255)

                               cACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTa                                                       >  1:3952707/1‑48 (MQ=11)

                                aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca                                                               >  1:2807810/1‑39 (MQ=33)

                                aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                                       <  1:969170/47‑1 (MQ=38)

                                aCTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGa                                                   >  1:2917911/1‑51 (MQ=11)

                                aCTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                                  <  1:2089205/68‑1 (MQ=11)

                                aCTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                                  <  1:2326521/68‑1 (MQ=11)

                                 cTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                    <  1:1382011/49‑1 (MQ=38)

                                 cTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTa                                        <  1:3600746/61‑1 (MQ=35)

                                  ttCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                                         <  1:4268354/43‑1 (MQ=38)

                                  ttCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACa                                                     >  1:1420254/1‑47 (MQ=38)

                                   tCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                    <  1:4068785/47‑1 (MQ=38)

                                   tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcc                                            <  1:1580560/55‑1 (MQ=11)

                                   tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                                             >  1:1611008/1‑54 (MQ=11)

                                    cTGATACCTCCAGCATGCCTCACAGCACACCTTca                                                               >  1:4519058/1‑35 (MQ=33)

                                    cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa                                                  >  1:3688140/1‑48 (MQ=38)

                                    cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                            <  1:3653045/70‑1 (MQ=40)

                                      gATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                              >  1:4176682/1‑66 (MQ=11)

                                        tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg                       <  1:3799113/71‑1 (MQ=255)

                                        tACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                                         >  1:3639210/1‑37 (MQ=14)

                                          ccTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTc                     >  1:3164847/1‑71 (MQ=255)

                                            tCCAGCATACCTCACAGTACACCTTCACAGGTTTACAg                                                    <  1:4046631/38‑1 (MQ=25)

                                            tCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                                     >  1:2783897/1‑53 (MQ=11)

                                            tCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                                     >  1:2619381/1‑53 (MQ=11)

                                            tCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                                     >  1:2140870/1‑53 (MQ=11)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                  >  1:548387/1‑71 (MQ=255)

                                              cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                                >  1:132964/1‑56 (MQ=35)

                                              cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                                >  1:334127/1‑56 (MQ=35)

                                              cAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTccc                                           >  1:2076083/1‑45 (MQ=11)

                                                gCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAacac                             >  1:2717999/1‑57 (MQ=40)

                                                gCATGCCTCACAGAACACCTTCACAGGCTTACAGAACGCTcc                                            >  1:935534/1‑42 (MQ=255)

                                                  aTGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCg              >  1:1306844/1‑70 (MQ=255)

                                                    accTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                              <  1:1864686/51‑2 (MQ=255)

                                                      cTCACAGGCCACCTTCACAGGCTTACAGAACGCTc                                             >  1:4392967/1‑35 (MQ=14)

                                                       tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGc             <  1:206061/66‑1 (MQ=255)

                                                       tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCtt        >  1:247150/1‑71 (MQ=255)

                                                         acaGCACACCTTCCCAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTc       <  1:846620/70‑1 (MQ=255)

                                                         acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAacac                             >  1:1296735/1‑48 (MQ=255)

                                                         acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCg      <  1:1472268/71‑1 (MQ=255)

                                                          caGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCgg     >  1:1569254/1‑71 (MQ=255)

                                                           aGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                            >  1:689444/1‑47 (MQ=255)

                                                            gCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATa                          <  1:3187845/48‑1 (MQ=255)

                                                             cacacaTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgt                        >  1:1045016/1‑49 (MQ=255)

                                                             cacaCCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAg           >  1:644922/1‑62 (MQ=255)

                                                             cacaCCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTGc  <  1:796198/71‑1 (MQ=255)

                                                             cacaCCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTGc  <  1:3227092/71‑1 (MQ=255)

                                                             cacaCCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTGc  <  1:3483478/71‑1 (MQ=255)

CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTGC  >  minE/2359815‑2359946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`