| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,373,313 | G→T | 11.4% | R105S (CGC→AGC) | ubiD ← | 3‑octaprenyl‑4‑hydroxybenzoate decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,373,313 | 0 | G | T | 11.4% | 183.4 / 6.3 | 61 | R105S (CGC→AGC) | ubiD | 3‑octaprenyl‑4‑hydroxybenzoate decarboxylase |
| Reads supporting (aligned to +/- strand): ref base G (27/27); new base T (3/4); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
ACCACGCAGCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCACGCAGCGCCGAAA > minE/2373245‑2373381 | accacGCAGCCGCTTTGTCGGCATGTTCAATAATTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGa < 1:2228231/71‑1 (MQ=255) ccacGCAGCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGa < 1:2796405/70‑1 (MQ=255) ccacGCAGCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGa > 1:1537850/1‑70 (MQ=255) cacGCAGCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCgg < 1:4214923/68‑1 (MQ=255) acGCAGCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAAc > 1:2194837/1‑71 (MQ=255) acGCAGCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGATTATCAAACAGGTCGCGGAAAc > 1:3332525/1‑71 (MQ=255) cGCAGCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAAcc > 1:1136171/1‑71 (MQ=255) cGCAGCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAAcc > 1:2406037/1‑71 (MQ=255) cGCAGCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAAcc < 1:3490006/71‑1 (MQ=255) aGCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCttt < 1:2053695/71‑1 (MQ=255) aGCCGCTTTGTCGGCATGTTCAATAATTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCttt < 1:3439768/71‑1 (MQ=255) gCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCtttt < 1:3989228/71‑1 (MQ=255) ccGCTTTGTCGGCATGTTCAATACTTGCTTAAACTG‑GGCAGTTTATCAAACAGGTCGCGGAAACCTTTTgg > 1:1759359/1‑71 (MQ=255) cGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTgg > 1:3833974/1‑71 (MQ=255) tGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTggcggc < 1:2555951/70‑1 (MQ=255) tGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTggcggc < 1:2550582/70‑1 (MQ=255) tCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCt < 1:970109/59‑1 (MQ=255) aTGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGcggctccggctc > 1:3300556/1‑71 (MQ=255) cAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCttt > 1:3803461/1‑69 (MQ=255) cAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCttt > 1:1475655/1‑69 (MQ=255) aaTACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAg < 1:3868877/71‑1 (MQ=255) tACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGaa < 1:2798217/42‑1 (MQ=255) tACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGtggctccggc < 1:3415860/61‑1 (MQ=255) ccttgcccggcgttaatgatttgccCAAACAGGTCGCTGaaa > 1:1435596‑M2/26‑42 (MQ=255) ccttgcccggcgttaatgatttgccCAAACAGGTCGCTGa < 1:2202077‑M2/15‑1 (MQ=255) aCTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGaaa > 1:378812/1‑71 (MQ=255) cTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTgg > 1:1911739/1‑49 (MQ=255) cTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTg < 1:459160/48‑1 (MQ=255) cTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTg < 1:1853750/48‑1 (MQ=255) cTTGCTTAAACTGCGGCAGTTTATCAAACAGGGCGCGGAAACCt < 1:2714533/44‑1 (MQ=255) ttgcccggcgttaatgatttgccCAAACAGGTCGCTGaaa > 1:282622‑M2/24‑40 (MQ=255) ttgcccggcgttaatgatttgccCAAACAGGTCGCTGaaa < 1:4694086‑M2/17‑1 (MQ=255) ttgcccggcgttaatgatttgccCAAACAGGTCGCTGa < 1:183108‑M2/15‑1 (MQ=255) tgcccggcgttaatgatttgccCAAACAGGTCGCTGaa > 1:4628403‑M2/23‑38 (MQ=255) tgcccggcgttaatgatttgccCAAACAGGTCGCTGa < 1:1307213‑M2/15‑1 (MQ=255) gCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGaaa > 1:2970999/1‑67 (MQ=255) gCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGc > 1:225623/1‑70 (MQ=255) aaaCTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGaaa > 1:1948559/1‑63 (MQ=255) aaaCTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCaat > 1:1329353/1‑70 (MQ=255) aCTGCGGCAGTTTATCAAACAGGTCGCGTAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCaataat < 1:4589126/71‑1 (MQ=255) cTGCGGCAGTTTATCAAACAGGTCGCGGAAACCtttt < 1:3510144/37‑1 (MQ=255) gCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGaaa > 1:2974262/1‑58 (MQ=255) ggCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTAcc < 1:216303/71‑1 (MQ=255) tttATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGctct < 1:1930010/43‑1 (MQ=255) tttATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACtt < 1:1838317/71‑1 (MQ=255) ttATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTAcc < 1:2915724/65‑1 (MQ=255) tataAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCa > 1:1875606/1‑71 (MQ=255) tATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTc > 1:886822/1‑44 (MQ=255) tATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTc > 1:4341359/1‑44 (MQ=255) tATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCaataa > 1:245774/1‑58 (MQ=255) tATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTc < 1:1348547/70‑1 (MQ=255) tATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCa > 1:3982837/1‑71 (MQ=255) tCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGaaa > 1:4454827/1‑47 (MQ=255) tCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCACg < 1:2403261/71‑1 (MQ=255) tCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCACg > 1:70173/1‑71 (MQ=255) cAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCACg < 1:3504913/70‑1 (MQ=255) aaaCAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCa < 1:1639995/61‑1 (MQ=255) cAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCCCGCAgcg > 1:889348/1‑71 (MQ=255) cAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCACGCAgcg > 1:2762416/1‑71 (MQ=255) ggTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGa < 1:4450637/38‑1 (MQ=255) gTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACt < 1:2302146/58‑1 (MQ=255) gTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCACGCAGCGcc > 1:93870/1‑70 (MQ=255) cgcgGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCaat > 1:1688794/1‑44 (MQ=255) gcgGAAACCTTTTGGCGTCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCACGCAGCGCCGaaa > 1:2931629/1‑71 (MQ=255) gcgGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCACGCAGCGCCGaa < 1:2280624/70‑1 (MQ=255) | ACCACGCAGCCGCTTTGTCGGCATGTTCAATACTTGCTTAAACTGCGGCAGTTTATCAAACAGGTCGCGGAAACCTTTTGGCGGCTCCGGCTCTTTCAGAAACGCCAATAATTTACCAACTTCACGCAGCGCCGAAA > minE/2373245‑2373381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |