| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,398,935 | C→T | 5.6% | E566K (GAG→AAG) | uvrD ← | DNA‑dependent ATPase I and helicase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,398,935 | 0 | C | T | 5.6% | 278.2 / 3.8 | 89 | E566K (GAG→AAG) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base C (38/46); new base T (2/3); total (40/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CTTCATCCAGCGACATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTCACCT > minE/2398866‑2399005 | cTTCATCCAGCGACATCTGGCTTGGGAACATGCCCTCTTTCCATACCAACGATAAACACCTGCGGGAACTc < 1:3245684/71‑1 (MQ=255) ttCATCCAGCGACATCTGGCTTGGGAACATGCCCTCTTTCCATACCAACGATAAACACCTGCGGGAACTc < 1:4648738/70‑1 (MQ=255) tCATCCAGCGACATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAg > 1:2539370/1‑71 (MQ=255) cATCCAGCGACATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAgg > 1:686058/1‑71 (MQ=255) cATCCAGCGACATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAgg > 1:4335218/1‑71 (MQ=255) aTCCAGCGACATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGc > 1:4628640/1‑71 (MQ=255) ccAGCGACATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCa > 1:2613351/1‑66 (MQ=255) ccAGCGACATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCa > 1:4402618/1‑66 (MQ=255) ccAGCGACATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCa > 1:4388795/1‑66 (MQ=255) tcGACATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTc > 1:4443449/2‑71 (MQ=255) gCGACATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTc < 1:229470/71‑1 (MQ=255) cATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCg < 1:1064174/71‑1 (MQ=255) cATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCg < 1:3836506/71‑1 (MQ=255) aTCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGa < 1:4616214/71‑1 (MQ=255) aTCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGa < 1:3859583/71‑1 (MQ=255) aTCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCAGGAACTCCAGGCCTTTCGCCg < 1:2700054/70‑1 (MQ=255) tCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTTCAGGCCTTTCGcc < 1:2136372/68‑1 (MQ=255) tCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCtt < 1:1547900/63‑1 (MQ=255) gCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAgtgt < 1:3944070/70‑1 (MQ=255) gCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAg < 1:2289197/67‑1 (MQ=255) gCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTa < 1:3431095/71‑1 (MQ=255) gggAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCg < 1:3985704/70‑1 (MQ=255) ggAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGt < 1:2699141/70‑1 (MQ=255) aCATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcatc < 1:3559474/71‑1 (MQ=255) aCATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcatc < 1:4496791/71‑1 (MQ=255) aCATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcatc < 1:2440755/71‑1 (MQ=255) aCATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcat > 1:1417298/1‑70 (MQ=255) aCATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcat > 1:1344609/1‑70 (MQ=255) aCATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcat > 1:4498732/1‑70 (MQ=255) cccTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTc > 1:997116/1‑48 (MQ=255) cccTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTg < 1:4691528/71‑1 (MQ=255) ctc‑tTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCa > 1:3995093/1‑71 (MQ=255) tc‑tTCCATACCAACGATAAACACCTGCGGGAACTTCAg < 1:3237463/38‑1 (MQ=255) tc‑tTCCATACCAACGATAAACACCTGCGGGAACTTCAg < 1:3782865/38‑1 (MQ=255) c‑tTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCtt > 1:805704/1‑42 (MQ=255) c‑tTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTc > 1:2932281/1‑44 (MQ=255) ttCCATACCAACGATAAACACCTGCGGGAACTCCa > 1:3451677/1‑35 (MQ=255) ttCCATACCAACGATAAACACCTGCGGGAACTCCAGGCGTTTCGCCGAGTGTAGCGTCATCAACTGCACCg > 1:1133556/1‑71 (MQ=255) ttCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAAc > 1:2893230/1‑64 (MQ=255) ttCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCg > 1:1189787/1‑71 (MQ=255) ccATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCAcc < 1:639514/68‑1 (MQ=255) ccATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCa > 1:1737541/1‑71 (MQ=255) aTACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATc < 1:2278140/71‑1 (MQ=255) aTACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATc < 1:2063099/71‑1 (MQ=255) tACCAACGATAAACACCTGCGGGAACTCCAGGCCtt < 1:2495751/36‑1 (MQ=255) tACCAACGATAAACACCTGCGGGAACTCCAGGCCtt < 1:551660/36‑1 (MQ=255) tACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtc < 1:211152/53‑1 (MQ=255) tACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCa < 1:1515800/68‑1 (MQ=255) tACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCa < 1:4661710/68‑1 (MQ=255) tACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATcc > 1:681333/1‑71 (MQ=255) aCCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCt > 1:3952628/1‑71 (MQ=255) ccAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGa > 1:854351/1‑41 (MQ=255) ccAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCg < 1:1942177/49‑1 (MQ=255) aaCGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcatca < 1:4050397/53‑1 (MQ=255) aCGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtca > 1:325341/1‑49 (MQ=255) aCGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCa < 1:17413/63‑1 (MQ=255) aCGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGc > 1:1217024/1‑69 (MQ=255) cGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcatc < 1:530259/50‑1 (MQ=255) gATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcatc < 1:625458/49‑1 (MQ=255) gATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAgg > 1:2611243/1‑71 (MQ=255) aTAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAgg > 1:1883819/1‑70 (MQ=255) tAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcatc < 1:1390460/47‑1 (MQ=255) tAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcatc < 1:1359118/47‑1 (MQ=255) tAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTa < 1:104661/71‑1 (MQ=255) aaaCACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCaa > 1:1328215/1‑48 (MQ=255) aaaCACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTAt > 1:2830937/1‑71 (MQ=255) aaaCACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTAt > 1:1561750/1‑71 (MQ=255) aaCACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAAc > 1:4519551/1‑48 (MQ=255) aaCACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGc < 1:4290243/51‑1 (MQ=255) aaCACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCa < 1:205685/57‑1 (MQ=255) acacCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGtcatc < 1:283924/44‑1 (MQ=255) acacCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATcc < 1:3669156/71‑1 (MQ=255) cacCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCg > 1:877681/1‑71 (MQ=255) acCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGc < 1:435655/71‑1 (MQ=255) cTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCt > 1:3940927/1‑71 (MQ=255) gCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATc > 1:1501149/1‑52 (MQ=255) gCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGc > 1:2081446/1‑71 (MQ=255) ggAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCg < 1:1633914/45‑1 (MQ=255) ggAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCt < 1:1871309/71‑1 (MQ=255) aaCTTCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCtt > 1:2999698/1‑70 (MQ=255) aaCTTCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCtt > 1:3406293/1‑70 (MQ=255) aaCTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTc < 1:1529144/71‑1 (MQ=255) aaCTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTc < 1:3048908/71‑1 (MQ=255) aaCTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTAACCGCCTGCCCTTc < 1:68940/71‑1 (MQ=255) aCTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTc < 1:1899822/70‑1 (MQ=255) aCTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTCa > 1:1923991/1‑71 (MQ=255) cTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTa < 1:1206046/55‑1 (MQ=255) cTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATcc < 1:2569599/58‑1 (MQ=255) tCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCtt < 1:3343603/67‑1 (MQ=255) tCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTCAcc > 1:3667140/1‑71 (MQ=255) tCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTCAcc > 1:1621652/1‑71 (MQ=255) ccAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATc > 1:323546/1‑55 (MQ=255) ccAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGcc > 1:203613/1‑59 (MQ=255) ccAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTCACCt > 1:2161224/1‑71 (MQ=255) ccAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTCACCt > 1:1978110/1‑71 (MQ=255) ccAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTCACCt > 1:4563394/1‑71 (MQ=255) ccAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTCACCt > 1:4318078/1‑71 (MQ=255) | CTTCATCCAGCGACATCTGGCTTGGGAACATGCCCTC‑TTCCATACCAACGATAAACACCTGCGGGAACTCCAGGCCTTTCGCCGAGTGTAGCGTCATCAACTGCACCGCATCCTGCCAGGTATCCGCCTGCCCTTCACCT > minE/2398866‑2399005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |