| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,482,946 | A→G | 5.0% | intergenic (‑83/+185) | mdtL ← / ← trmE | multidrug efflux system protein/GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,482,946 | 0 | A | G | 5.0% | 440.3 / 11.0 | 158 | intergenic (‑83/+185) | mdtL/trmE | multidrug efflux system protein/GTPase |
| Reads supporting (aligned to +/- strand): ref base A (71/79); new base G (3/5); total (74/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
Only 100 of 172 total aligned reads displayed.
CTGATAGTAAGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTAAATGCTTTTTTTA > minE/2482877‑2483013 | cTGATAGTAAGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTa < 1:1212865/70‑1 (MQ=255) gATAGTAAGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTc > 1:2169848/1‑71 (MQ=255) aTAGTAAGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTa < 1:2328222/67‑1 (MQ=255) aTAGTAAGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTa < 1:1593620/67‑1 (MQ=255) aTAGTAAGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCt > 1:1970944/1‑71 (MQ=255) aGTAAGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTg > 1:4327219/1‑70 (MQ=255) gTAAGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTg > 1:1798469/1‑69 (MQ=255) tAAGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGaaa < 1:495838/71‑1 (MQ=255) aaGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTa > 1:574248/1‑62 (MQ=255) aaGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAAt > 1:2975641/1‑71 (MQ=255) aaGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAAt > 1:1759198/1‑71 (MQ=255) aGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAAtt > 1:3940606/1‑71 (MQ=255) ggAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTg < 1:2524554/71‑1 (MQ=255) ggAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTg < 1:3499846/71‑1 (MQ=255) tGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGa < 1:1485655/62‑1 (MQ=255) tGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGttt > 1:2179466/1‑70 (MQ=255) tGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGttt > 1:2870892/1‑70 (MQ=255) cACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTaaat > 1:3448693/1‑61 (MQ=255) cACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTaaa > 1:4523437/1‑71 (MQ=255) cACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTaa < 1:741733/70‑1 (MQ=255) tATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTa > 1:4624953/1‑50 (MQ=255) tATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAAt > 1:1402404/1‑68 (MQ=255) aTCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAAtgtg > 1:1459167/1‑70 (MQ=255) aTCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAAtgtg > 1:412197/1‑70 (MQ=255) aTCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAAtgtg > 1:4218191/1‑70 (MQ=255) aTCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAAtgtg > 1:4375421/1‑70 (MQ=255) aTCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAAtgtg < 1:364835/70‑1 (MQ=255) aTCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAAt > 1:2269181/1‑67 (MQ=255) aTCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAAt > 1:713262/1‑67 (MQ=255) tCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTGTTCTGAAATTGTTTAAATGTgaa > 1:1519062/1‑71 (MQ=255) tCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAAt > 1:2856775/1‑66 (MQ=255) tCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTga < 1:2610937/70‑1 (MQ=255) tCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTga < 1:769786/70‑1 (MQ=255) cTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTg > 1:4025987/1‑51 (MQ=255) cTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTGAATGTgaat < 1:826778/70‑1 (MQ=255) tCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTaa < 1:4669770/61‑1 (MQ=255) cGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTa > 1:1421764/1‑44 (MQ=255) cGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTa > 1:1074701/1‑44 (MQ=255) cGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCt > 1:2902471/1‑48 (MQ=255) gCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTgaatcgaa > 1:263555/1‑71 (MQ=255) gCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTgaatcg < 1:3074661/69‑1 (MQ=255) cAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGa < 1:3369048/48‑1 (MQ=255) cAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTgaatcgaat < 1:678669/71‑1 (MQ=255) aGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAAtt > 1:3798242/1‑50 (MQ=255) aGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTga < 1:4635564/62‑1 (MQ=255) aGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATca < 1:2242043/71‑1 (MQ=255) gACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATcac < 1:1093199/71‑1 (MQ=255) acacGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTATATGTGAATCGAATcac > 1:2596702/1‑70 (MQ=255) acacGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATcaca < 1:2486703/71‑1 (MQ=255) acGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGaaa > 1:1784541/1‑44 (MQ=255) acGAGGCTACATTATTACACTCAGAGTTTTTGTATATTCTGAAATTGTTTAAAt < 1:1069490/54‑1 (MQ=255) cGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATc < 1:2080440/71‑1 (MQ=255) cGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATc < 1:3824381/71‑1 (MQ=255) ggCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGtt > 1:2655443/1‑71 (MQ=255) ggCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGtt < 1:191391/71‑1 (MQ=255) gCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATca < 1:3426979/62‑1 (MQ=255) gCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTc < 1:1011145/71‑1 (MQ=255) cTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTgaatcgaatga < 1:873842/61‑3 (MQ=255) cTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCg > 1:2612393/1‑71 (MQ=255) tACATTATTACACTCAGAGTTTTTGTCTATTCTGAAAtt > 1:3723515/1‑39 (MQ=255) tACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCgg > 1:785882/1‑71 (MQ=255) agattattACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTa < 1:1005800/41‑1 (MQ=255) cATTATTACACTCAGAGTTTTTGTCTATTCTGaaa < 1:3474198/35‑1 (MQ=255) cATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTaaa > 1:1524632/1‑44 (MQ=255) cATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCggg > 1:4470211/1‑70 (MQ=255) cATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGATTAAATGTGAATCGAATCACAATCGTTCggg > 1:2973548/1‑70 (MQ=255) attattACCCTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCTTTCgg > 1:644141/1‑68 (MQ=255) attattACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCggg > 1:1630032/1‑69 (MQ=255) ttattaCACTCAGAGTTTTTGTCTATTCTGAAATTGttt > 1:1118040/1‑39 (MQ=255) ttattaCACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGt < 1:2572134/63‑1 (MQ=255) tattaCACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCggg > 1:2719984/1‑67 (MQ=255) tattaCACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAg > 1:3972142/1‑70 (MQ=255) attaCACTCAGAGTTTTTGTCTGTTCTGAAATTGTTTaaa > 1:2785136/1‑40 (MQ=255) attaCACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGGGc < 1:2011230/70‑1 (MQ=255) attaCACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGc > 1:2340480/1‑70 (MQ=255) ttaCACTCAGAGTTTTTGTCTATTCTGAAATTGTTTa < 1:1257205/37‑1 (MQ=255) ttaCACTCAGAGTTTTTGTCTATTCTGAAATTGTTTa < 1:3772862/37‑1 (MQ=255) ttaCACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCaa < 1:609735/71‑1 (MQ=255) taCACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCaa > 1:1182048/1‑70 (MQ=255) acTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATAtt < 1:3249286/71‑1 (MQ=255) acTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATAtt < 1:2359358/71‑1 (MQ=255) cTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTa < 1:3246143/71‑1 (MQ=255) agagTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCaa > 1:734843/1‑63 (MQ=255) agagTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTAAAt > 1:3608370/1‑71 (MQ=255) tttttGTCTATTCTGAAATTGTTTAAATGTgaatcg < 1:447933/36‑1 (MQ=255) tttttGTCTATTCTGAAATTGTTTAAATGTGAATCGAATca < 1:2116635/41‑1 (MQ=255) tttttGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGtt < 1:1631959/49‑1 (MQ=255) tttttGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGt < 1:1200473/48‑1 (MQ=255) tttttGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTa < 1:1840088/64‑1 (MQ=255) tttttGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTa < 1:2187551/64‑1 (MQ=255) tttttGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTAAATGCtt < 1:4197101/71‑1 (MQ=255) tttGTCTATTCTGAAATTGTTTAAATGTGAATCGAATcaca > 1:2923164/1‑41 (MQ=255) tttGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTa < 1:155979/62‑1 (MQ=255) tttGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTAAATGCtttt > 1:4076386/1‑71 (MQ=255) ttGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACaa < 1:2102781/41‑1 (MQ=255) tCTGTTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTAAATGCTTTTTTTa < 1:2693167/71‑1 (MQ=255) tCTGTTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTAAATGCTTTTTTTa < 1:1034149/71‑1 (MQ=255) tCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTAAATGCTTTTTTTa < 1:1360686/71‑1 (MQ=255) cTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCggga > 1:1287073/1‑46 (MQ=255) tATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCg < 1:1975782/39‑1 (MQ=255) | CTGATAGTAAGGAATGCACTTATCCTCGCAAGACACGAGGCTACATTATTACACTCAGAGTTTTTGTCTATTCTGAAATTGTTTAAATGTGAATCGAATCACAATCGTTCGGGGAGCAATATTAAATGCTTTTTTTA > minE/2482877‑2483013 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |