BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,513,919	T→C	5.9%	F371L (TTT→CTT)	gltS →	glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,513,919	0	T	C	5.9%	274.6 / 7.3	102	F371L (TTT→CTT)	gltS	glutamate transporter
Reads supporting (aligned to +/- strand): ref base T (46/50); new base C (4/2); total (50/52)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

Only 100 of 120 total aligned reads displayed.

GGTGCAACGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGTCAATGCGCTGGTAATTAAGTTGTA  >  minE/2513850‑2513986

ggTGCAACGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGtt                                                                    <  1:1840327/71‑1 (MQ=255)

 gTGCAACGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGttt                                                                   <  1:1915800/71‑1 (MQ=255)

    cAACGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGttttt                                                                 <  1:899438/70‑1 (MQ=255)

    cAACGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtg                                                                <  1:436520/71‑1 (MQ=255)

    cAACGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtg                                                                <  1:1854598/71‑1 (MQ=255)

     aaCGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtgg                                                               <  1:1807951/71‑1 (MQ=255)

     aaCGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtg                                                                <  1:3576079/70‑1 (MQ=255)

      aCGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGttttt                                                                 >  1:3876768/1‑68 (MQ=255)

      aCGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtggt                                                              <  1:4665745/71‑1 (MQ=255)

       cGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtggtg                                                             >  1:4593811/1‑71 (MQ=255)

       cGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCAGTCGCACATGGCGTTTTtggtg                                                             >  1:4633229/1‑71 (MQ=255)

        gCCAAGGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtggt                                                              >  1:747667/1‑69 (MQ=255)

        gCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtggtgg                                                            >  1:2707136/1‑71 (MQ=255)

          cAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtggt                                                              <  1:921177/67‑1 (MQ=255)

          cAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGgtggtg                                                          <  1:3246652/71‑1 (MQ=255)

            aCGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGcc                                                        <  1:3735219/71‑1 (MQ=255)

            aCGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGcc                                                        <  1:3605495/71‑1 (MQ=255)

              ggCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGa                                                      <  1:4226354/71‑1 (MQ=255)

               gCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGCTTTTGGTGGTGCCGAt                                                     >  1:2473879/1‑71 (MQ=255)

                  aTCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGt                                                  <  1:3504363/71‑1 (MQ=255)

                  aTCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGt                                                  <  1:3093624/71‑1 (MQ=255)

                  aTCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGt                                                  <  1:2801055/71‑1 (MQ=255)

                   tCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtggtg                                                             >  1:399766/1‑59 (MQ=255)

                   tCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtggtg                                                             >  1:1064135/1‑59 (MQ=255)

                    cGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtgg                                                               >  1:85844/1‑56 (MQ=255)

                    cGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCg                                                >  1:1721716/1‑71 (MQ=255)

                     gCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCgg                                               >  1:3503533/1‑71 (MQ=255)

                      ccAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtgg                                                               <  1:3339588/54‑1 (MQ=255)

                       cAACATGCAGGCGATCTCTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTg                                             >  1:2534990/1‑71 (MQ=255)

                       cAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGttttt                                                                 >  1:868278/1‑51 (MQ=255)

                       cAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTg                                             <  1:3057608/71‑1 (MQ=255)

                        aaCATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGc                                            >  1:2420605/1‑71 (MQ=255)

                        aaCATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGc                                            >  1:1927831/1‑71 (MQ=255)

                         aCATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGCTTTTGGTGGTGCCGATg                                                    <  1:2849549/62‑1 (MQ=255)

                          cATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGt                                          >  1:1608078/1‑71 (MQ=255)

                           aTGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGtt                                         >  1:1330061/1‑71 (MQ=255)

                           aTGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGtt                                         >  1:2036499/1‑71 (MQ=255)

                           aTGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGCTTTTGGTGGTGCCGATGGTCGGTGCGt                                          >  1:3192844/1‑70 (MQ=255)

                              cAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCtt                                      <  1:2916683/71‑1 (MQ=255)

                              cAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCt                                       <  1:152228/70‑1 (MQ=255)

                               aGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCttt                                     <  1:294584/71‑1 (MQ=255)

                                ggCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGcc                                                        <  1:1591433/51‑1 (MQ=255)

                                 gCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGcc                                                        <  1:1141260/50‑1 (MQ=255)

                                  cGATCACTTAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATc                                  <  1:593417/71‑1 (MQ=255)

                                  cGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATc                                  <  1:340675/71‑1 (MQ=255)

                                    aTCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGt                                              <  1:2280781/57‑1 (MQ=255)

                                     tCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTtggt                                                              <  1:1853967/40‑1 (MQ=255)

                                     tCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGat                               <  1:2944594/71‑1 (MQ=255)

                                     tCACTGAACGCTTTGGCCCGTCGCACATGGCGCTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGa                                >  1:2220268/1‑70 (MQ=255)

                                     tCACTGAACGCTTTGGACCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGat                               <  1:4290501/71‑1 (MQ=255)

                                      cACTGACCGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGc                                            >  1:2126481/1‑57 (MQ=255)

                                      cACTGAACGCTTTGGCCCGTCGCACATGGCGttttt                                                                 >  1:371555/1‑36 (MQ=255)

                                      cACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGAt                                                     >  1:844272/1‑48 (MQ=255)

                                      cACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATc                                  >  1:2453695/1‑67 (MQ=255)

                                      cACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGata                              >  1:1939977/1‑71 (MQ=255)

                                      cACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGata                              >  1:634976/1‑71 (MQ=255)

                                      cACTGAACGCTTTGGCCCGTCGCACATGGCGCTTTtggtg                                                             >  1:723126/1‑40 (MQ=255)

                                         tGAACGCTTTGGCCCGTCGCACATGGCGTTTTtggt                                                              <  1:838232/36‑1 (MQ=255)

                                          gAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGt                          >  1:2117622/1‑71 (MQ=255)

                                          gAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGt                          >  1:4163733/1‑71 (MQ=255)

                                          gAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGt                          >  1:3566236/1‑71 (MQ=255)

                                           aaCGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTAt                                   >  1:893640/1‑61 (MQ=255)

                                           aaCGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGt                          >  1:4085945/1‑70 (MQ=255)

                                           aaCGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGt                          <  1:2132499/70‑1 (MQ=255)

                                           aaCGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGTc                         <  1:1022409/71‑1 (MQ=255)

                                           aaCGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGTc                         <  1:3761344/71‑1 (MQ=255)

                                           aaCGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCg                           >  1:2255325/1‑69 (MQ=255)

                                           aaCGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGt                          >  1:1909405/1‑70 (MQ=255)

                                           aaCGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTc                         <  1:1048374/71‑1 (MQ=255)

                                           aaCGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTc                         <  1:4173139/71‑1 (MQ=255)

                                              gCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTc                         <  1:639873/68‑1 (MQ=255)

                                               cTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCg                                                       <  1:4234831/37‑1 (MQ=255)

                                                  tGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATAGATATCGTCAATgcgc                  >  1:2539836/1‑71 (MQ=255)

                                                  tGGCCCGTCGCACATGGCGTTCCTGGTCGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGTCAATgc                    <  1:3914664/69‑1 (MQ=255)

                                                   ggCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTc                         <  1:1212282/63‑1 (MQ=255)

                                                    gCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGTTGCGTTCTTTATc                                  <  1:4263129/53‑1 (MQ=255)

                                                      ccGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTc                         <  1:277413/60‑1 (MQ=255)

                                                        gTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTCAATGCGCTGGTaa            >  1:1004043/1‑71 (MQ=255)

                                                        gTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTCAATGCGCTGGTaa            >  1:1029550/1‑71 (MQ=255)

                                                        gTCGCACATGGCGCTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGTCAATGTGCTGGTaa            <  1:2266904/71‑1 (MQ=255)

                                                         tCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTCAATGCGCTGGTAAt           >  1:1106967/1‑71 (MQ=255)

                                                         tCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTCAATGCGCTGGTAAt           >  1:698914/1‑71 (MQ=255)

                                                           gCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGTCAATGCGCTGGTAAtt          <  1:2672970/70‑1 (MQ=255)

                                                            cacaTGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGTCAATGCGCTGGTAATTaa        <  1:3167832/71‑1 (MQ=255)

                                                            cacaTGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTc                         <  1:1410930/54‑1 (MQ=255)

                                                             acaTGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGTCAATGCGCTGGTAATTAAg       >  1:4536674/1‑71 (MQ=255)

                                                               aTGGCGTTTTTGGTGGTGCCGATTGTCGGTGCGTTCt                                       <  1:1996027/37‑1 (MQ=255)

                                                                tGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTa                                    <  1:2260708/39‑1 (MQ=255)

                                                                  gcgcTTTTGGTGGTGCCGATGGTCGGTGCGTTCtt                                      <  1:4470031/35‑1 (MQ=255)

                                                                  gCGTTTTTGTTGGTGCCGATGGTCGGTGCGTTCTTTATCGat                               >  1:4554227/1‑42 (MQ=255)

                                                                  gCGTTTTTGGTGGTGCCGATGGTCGGTTCGTTCTTTATCGATATCGTCAATGCGCTGGTaa            >  1:4260881/1‑61 (MQ=255)

                                                                  gCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTCAATGCGCTGGTAATTAAGTTGTa  <  1:3216023/71‑1 (MQ=255)

                                                                     tttttGGTGGTGCCGATGGTCGGTGCGTTCTTTATc                                  <  1:4339223/36‑1 (MQ=255)

                                                                     tttttGGTGGTGCCGATGGTCGGTGCGTTCTTTATc                                  <  1:3391/36‑1 (MQ=255)

                                                                     tttttGGTGGTGCCGATGGTCGGTGCGTTCTTTATCg                                 <  1:4177093/37‑1 (MQ=255)

                                                                     tttttGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGTCAATGCGCTGGt              <  1:1901494/56‑1 (MQ=255)

                                                                     tttttGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTc                         <  1:513357/45‑1 (MQ=255)

                                                                     tttttGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTc                         <  1:1789926/45‑1 (MQ=255)

                                                                     tttttGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTc                         <  1:1620326/45‑1 (MQ=255)

                                                                     tttttGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATACCGTc                         <  1:3238668/45‑1 (MQ=255)

GGTGCAACGCCAACGGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTTTTGGTGGTGCCGATGGTCGGTGCGTTCTTTATCGATATCGTCAATGCGCTGGTAATTAAGTTGTA  >  minE/2513850‑2513986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`