BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,526,477	T→C	5.5%	M169V (ATG→GTG)	ttk ←	division inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,526,477	0	T	C	5.5%	302.8 / 6.2	109	M169V (ATG→GTG)	ttk	division inhibitor
Reads supporting (aligned to +/- strand): ref base T (59/44); new base C (4/2); total (63/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

GCGGCCAGCGGGCGTCAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCACCCTCACG  >  minE/2526408‑2526546

gCGGCCAGCGGGCGTCAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATa                                                                      <  1:346576/71‑1 (MQ=255)

 cGGCCAGCGGGCGTCAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCAt                                                                       <  1:4045830/69‑1 (MQ=255)

 cGGCCAGCGGGCGTCAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATAc                                                                     <  1:3876234/71‑1 (MQ=255)

      aGCGGGCGTCAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTca                                                                <  1:4648856/71‑1 (MQ=255)

          ggCGTCAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAg                                                             >  1:2650756/1‑70 (MQ=255)

          ggCGTCAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAg                                                             >  1:2784984/1‑70 (MQ=255)

            cGTCAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAg                                                             >  1:3223465/1‑68 (MQ=255)

              tCAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTcac                                                               >  1:3521704/1‑64 (MQ=255)

              tCAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGc                                                        <  1:4450584/71‑1 (MQ=255)

               cAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCACACCTTca                                                                >  1:3218198/1‑62 (MQ=255)

               cAAAATCATCCGTCGGCCGGTATTTAAATTTGCTGCGGACAAAACGTGACAGCACACCGTca                                                                >  1:449087/1‑62 (MQ=255)

                aaaaTCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGcc                                                       <  1:3059916/70‑1 (MQ=255)

                aaaaTCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGca                                                       <  1:1630932/70‑2 (MQ=255)

                aaaaTCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGc                                                        >  1:2573712/1‑69 (MQ=255)

                aaaaTCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGc                                                        >  1:2925076/1‑69 (MQ=255)

                aaaaTCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGc                                                        >  1:2930527/1‑69 (MQ=255)

                 aaaTCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAg                                                     >  1:1531955/1‑71 (MQ=255)

                    tcatcCGTCGGGCGGTATTTAAATTCGCTGCGGAGAAAACGTGACAGCATACCTTCACAGAAGGCCAGGAt                                                  <  1:2381362/71‑1 (MQ=255)

                     catcCGTCGGGCGGTATTTAAATTCGCTGCGTACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATc                                                 >  1:4241540/1‑71 (MQ=255)

                      atcCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATc                                                 <  1:2082556/70‑1 (MQ=255)

                        ccGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTgg                                              >  1:2187114/1‑71 (MQ=255)

                           tCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCtt                                           <  1:3963071/71‑1 (MQ=255)

                            cGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAgg                                                    <  1:599666/61‑1 (MQ=255)

                             gggCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTg                                          <  1:2435871/70‑1 (MQ=255)

                             gggCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGc                                         >  1:3214544/1‑71 (MQ=255)

                                cGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCcag                                      >  1:2751414/1‑71 (MQ=255)

                                  gTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCa                                                      <  1:137236/53‑1 (MQ=255)

                                  gTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGc                                         <  1:1712055/66‑1 (MQ=255)

                                   tATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCcagcag                                   <  1:2350614/71‑1 (MQ=255)

                                     tttAAATTCGCTGCGGACAAAACGTGACAGCATACCTTcaca                                                              <  1:738117/42‑1 (MQ=255)

                                     tttAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAgg                                                    <  1:4466563/52‑1 (MQ=255)

                                     tttAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAgg                                                    <  1:2479612/52‑1 (MQ=255)

                                     tttAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAgg                                                    <  1:879732/52‑1 (MQ=255)

                                     tttAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAgg                                                    <  1:1583006/52‑1 (MQ=255)

                                     tttAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCcagcag                                   >  1:2370491/1‑69 (MQ=255)

                                     tttAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAggg                                 >  1:4701103/1‑71 (MQ=255)

                                      ttAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGaa                                                           >  1:4371519/1‑44 (MQ=255)

                                      ttAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGTATCTGGCTTGCCAGCAGGGt                                >  1:4160553/1‑71 (MQ=255)

                                       tAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGaa                                                           >  1:1091225/1‑43 (MQ=255)

                                       tAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCtt                                           >  1:3903048/1‑59 (MQ=255)

                                       tAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCcag                                      >  1:3490604/1‑64 (MQ=255)

                                       tAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGtt                               >  1:1400062/1‑71 (MQ=255)

                                       tAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGtt                               >  1:330551/1‑71 (MQ=255)

                                       tAAATTCGCTGCGGACAAAACGTGACAGCACACATTCACAGAAGGCCAGGATc                                                 >  1:3661691/1‑53 (MQ=255)

                                         aaTTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGaa                                                           >  1:517042/1‑41 (MQ=255)

                                           ttCGCTGCGGACAAAACGTGACAGCACACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCAt                           <  1:4427138/71‑1 (MQ=255)

                                            tCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCa                            >  1:1536477/1‑69 (MQ=255)

                                            tCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATc                          >  1:1343606/1‑71 (MQ=255)

                                             cGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCg                         >  1:2775115/1‑71 (MQ=255)

                                             cGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCg                         >  1:483525/1‑71 (MQ=255)

                                             cGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCg                         >  1:3817916/1‑71 (MQ=255)

                                             cGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCg                         >  1:1899983/1‑71 (MQ=255)

                                              gCTGCGGACAAAACGTGACAGCATACCTTCACAGaa                                                           >  1:2817704/1‑36 (MQ=255)

                                              gCTGCGGACAAAACGTGACAGCATACCTTCACAGaa                                                           >  1:1655787/1‑36 (MQ=255)

                                              gCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGAt                                                  >  1:1958074/1‑45 (MQ=255)

                                               cTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCggt                       <  1:279339/71‑1 (MQ=255)

                                               cTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCggt                       <  1:533725/71‑1 (MQ=255)

                                                tGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCtt                                           >  1:2512360/1‑50 (MQ=255)

                                                 gCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCggtgg                     >  1:507213/1‑71 (MQ=255)

                                                 gCGGACAAAACGTGACAGAATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCggtgg                     >  1:3254441/1‑71 (MQ=255)

                                                  cGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCggtggt                    >  1:888775/1‑71 (MQ=255)

                                                  cGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCggtgg                     <  1:4436978/70‑1 (MQ=255)

                                                  cGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTACATCgg                        >  1:1304826/1‑67 (MQ=255)

                                                  cGGACAAAACGTGACAGCACACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCggtggt                    >  1:888284/1‑71 (MQ=255)

                                                   ggACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAggg                                 <  1:4199019/57‑1 (MQ=255)

                                                    gACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCggtgg                     >  1:2034411/1‑68 (MQ=255)

                                                    gACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCggtgg                     >  1:19060/1‑68 (MQ=255)

                                                     aCAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGc                                             <  1:1250604/43‑1 (MQ=255)

                                                     aCAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGc                                             <  1:1520970/43‑1 (MQ=255)

                                                     aCAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGc                                         <  1:4077191/47‑1 (MQ=255)

                                                     aCAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCcagca                                    >  1:519428/1‑52 (MQ=255)

                                                     aCAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCcag                                      <  1:1470827/50‑1 (MQ=255)

                                                     aCAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGgtgt                  >  1:1022263/1‑70 (MQ=255)

                                                     aCAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTa                 <  1:3413069/71‑1 (MQ=255)

                                                     aCAAAACGTGACAGCACACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTa                 <  1:1719424/71‑1 (MQ=255)

                                                      cAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCtt                                           <  1:955560/44‑1 (MQ=255)

                                                      cAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTaa                >  1:4268297/1‑71 (MQ=255)

                                                         aaCGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTc                             <  1:2028457/55‑1 (MQ=255)

                                                         aaCGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCgg                        >  1:2141095/1‑60 (MQ=255)

                                                         aaCGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTaa                >  1:466885/1‑68 (MQ=255)

                                                         aaCGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAAc               >  1:4560351/1‑69 (MQ=255)

                                                         aaCGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCt             >  1:3764480/1‑71 (MQ=255)

                                                         aaCGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCt             <  1:2596215/71‑1 (MQ=255)

                                                            gTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCa          <  1:2515377/71‑1 (MQ=255)

                                                            gTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCa          <  1:878170/71‑1 (MQ=255)

                                                                cAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCACCCt      >  1:460625/1‑71 (MQ=255)

                                                                 aGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCACCCt      >  1:896050/1‑70 (MQ=255)

                                                                  gCATACCTTCACAGAAGGCCAGGATCTGGCTTGCcag                                      <  1:4436214/37‑1 (MQ=255)

                                                                  gCATACCTTCACAGAAGGCCAGGATATGGCTTGGCAGCAGGGTTTCATCGGTGgtgt                  <  1:4629855/57‑1 (MQ=255)

                                                                   cATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCgg                        >  1:651347/1‑50 (MQ=255)

                                                                    aTACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGgtgt                  <  1:1265052/55‑1 (MQ=255)

                                                                    aTACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGgtgt                  <  1:1858415/55‑1 (MQ=255)

                                                                    aTACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCAcc        >  1:161882/1‑65 (MQ=255)

                                                                    aTACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCAcc        >  1:3934478/1‑65 (MQ=255)

                                                                    aTACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCACCCTCACg  <  1:2783118/71‑1 (MQ=255)

                                                                    aTACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCACCCTCACg  >  1:4356489/1‑71 (MQ=255)

                                                                    aTACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCACCCTCACg  >  1:1871010/1‑71 (MQ=255)

                                                                     tACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCACCCTCACg  >  1:4668288/1‑70 (MQ=255)

                                                                     tACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCACCCTCACg  >  1:1515267/1‑70 (MQ=255)

                                                                     tACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCACCCTCACg  >  1:3278182/1‑70 (MQ=255)

GCGGCCAGCGGGCGTCAAAATCATCCGTCGGGCGGTATTTAAATTCGCTGCGGACAAAACGTGACAGCATACCTTCACAGAAGGCCAGGATCTGGCTTGCCAGCAGGGTTTCATCGGTGGTGTAACCTTCACCCTCACG  >  minE/2526408‑2526546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`