| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,531,994 | T→C | 50.7% | L314L (TTA→TTG) | kdtA ← | 3‑deoxy‑D‑manno‑octulosonic‑acid transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,531,994 | 0 | T | C | 50.7% | 8.9 / 221.5 | 154 | L314L (TTA→TTG) | kdtA | 3‑deoxy‑D‑manno‑octulosonic‑acid transferase |
| Reads supporting (aligned to +/- strand): ref base T (37/39); new base C (38/40); total (75/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
Only 100 of 157 total aligned reads displayed.
CAGCGGATTATGCCCACCACGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAGGGGACTTCCCCTGAAGAGC > minE/2531925‑2532064 | cAGCGGATTATGCCCACCACGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGt > 1:711515/1‑70 (MQ=255) gCGGATTATGCCCACCACGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAAc < 1:2027605/71‑1 (MQ=255) cGGATTATGCCCACCACGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACa > 1:3776368/1‑71 (MQ=255) cGGATTATGCCCACCACGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACa > 1:1881103/1‑71 (MQ=255) cGGATTATGCCCACCACGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACa > 1:1137642/1‑71 (MQ=255) gATTATGCCCACCACGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATc < 1:182715/71‑1 (MQ=255) gATTATGCCCACCACGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATc < 1:3158350/71‑1 (MQ=255) ttATGCCCACCACGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACa > 1:3997874/1‑67 (MQ=255) tATGCCCACCACGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCaa > 1:3891478/1‑70 (MQ=255) ccacGTTCAACCAGTGAACGGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCAt > 1:3641849/1‑71 (MQ=255) ccacGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGc < 1:2600964/67‑1 (MQ=255) ccacGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCAt > 1:2399120/1‑71 (MQ=255) ccacGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCAt > 1:1123782/1‑71 (MQ=255) cacGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATc > 1:3421135/1‑71 (MQ=255) cacGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATc > 1:3121734/1‑71 (MQ=255) cacGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATc > 1:2319502/1‑71 (MQ=255) acGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATc > 1:1569376/1‑70 (MQ=255) cGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACa > 1:506054/1‑55 (MQ=255) cGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTc < 1:1899778/62‑1 (MQ=255) gTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTa < 1:3046682/71‑1 (MQ=255) cAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCg > 1:3688538/1‑71 (MQ=255) cAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCAATACAGTAACATCAACTCGCCCATCGTATCg > 1:4102533/1‑71 (MQ=255) aaCCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCGTACAGTAACATCa < 1:171921/53‑1 (MQ=255) ccAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGt < 1:2645015/64‑1 (MQ=255) cAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCaa > 1:1839474/1‑51 (MQ=255) cAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCaa > 1:111486/1‑51 (MQ=255) cAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCaa > 1:4543511/1‑51 (MQ=255) cAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCaa > 1:1609911/1‑51 (MQ=255) aGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCg < 1:15806/61‑1 (MQ=255) gTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCaa > 1:4449031/1‑43 (MQ=255) gAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACca > 1:1020958/1‑70 (MQ=255) gAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACca > 1:4321675/1‑70 (MQ=255) aaCCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCAt > 1:4241972/1‑55 (MQ=255) aaCCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACcaca < 1:3529213/71‑1 (MQ=255) aaCCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACcaca < 1:1738471/71‑1 (MQ=255) aaCCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACcaca < 1:2972861/71‑1 (MQ=255) aaCCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACcac > 1:4114520/1‑70 (MQ=255) aaCCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACcac < 1:3901735/70‑1 (MQ=255) aCCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACcaca < 1:1783174/70‑1 (MQ=255) aCCGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACcaca < 1:722036/70‑1 (MQ=255) cGCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGt < 1:1791278/55‑1 (MQ=255) gCCAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCt < 1:1024036/71‑1 (MQ=255) ccAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCg < 1:584882/57‑1 (MQ=255) ccAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTg > 1:796306/1‑71 (MQ=255) ccAACAAAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCa > 1:3698335/1‑60 (MQ=255) caacaaAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGc < 1:4030820/71‑1 (MQ=255) caacaaAGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGc < 1:3066702/71‑1 (MQ=255) aacaaAGGCGAGATCGGCAATGCCATACAGCAACAt > 1:944335/1‑36 (MQ=255) acaaAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGt > 1:2426182/1‑71 (MQ=255) caaAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTg > 1:3954883/1‑71 (MQ=255) caaAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTg > 1:86960/1‑71 (MQ=255) caaAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTg > 1:2164829/1‑71 (MQ=255) caaAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTg < 1:2791198/71‑1 (MQ=255) aaaGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTAt > 1:15204/1‑50 (MQ=255) aaaGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTAt > 1:2428921/1‑50 (MQ=255) aaGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCt > 1:1178104/1‑71 (MQ=255) aaGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCt < 1:4021700/71‑1 (MQ=255) aGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCg < 1:1917290/45‑1 (MQ=255) aGGCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCt < 1:2060332/63‑1 (MQ=255) ggCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtgg < 1:1208418/71‑1 (MQ=255) gCGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggt < 1:2768775/71‑1 (MQ=255) cGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGt < 1:3867727/43‑1 (MQ=255) cGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggtg < 1:3683089/71‑1 (MQ=255) cGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggtg < 1:4434590/71‑1 (MQ=255) cGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggtg < 1:4295044/71‑1 (MQ=255) cGAGATCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggt > 1:3194331/1‑70 (MQ=255) gagaTCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggtgg < 1:1815889/71‑1 (MQ=255) gagaTCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggtgg < 1:3540938/71‑1 (MQ=255) aTCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggt > 1:4285401/1‑66 (MQ=255) aTCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCt < 1:1512847/63‑1 (MQ=255) aTCGGCAATGCCATACAGTAACAT‑AACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCt < 1:4153627/62‑1 (MQ=255) aTCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggtgg < 1:4251291/68‑1 (MQ=255) aTCGGCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggtgg < 1:3183166/68‑1 (MQ=255) tCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAggg < 1:3592157/71‑1 (MQ=255) ggCAATGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAgggg < 1:1926811/70‑1 (MQ=255) aTGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAGGGGACTTc < 1:4580199/71‑1 (MQ=255) aTGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAGGGGACTTc > 1:3190999/1‑71 (MQ=255) tGCCATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAgggg < 1:3443497/65‑1 (MQ=255) gCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACaa > 1:855855/1‑44 (MQ=255) gCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAAc > 1:802301/1‑45 (MQ=255) ccATACAGCAACATCAACTCGCCCATCGTATCGCCAAcc > 1:2668380/1‑39 (MQ=255) ccATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTg > 1:1365504/1‑51 (MQ=255) ccATACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTg > 1:4400523/1‑51 (MQ=255) cATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTg < 1:212137/46‑1 (MQ=255) cATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTTCGTGCt > 1:3302455/1‑52 (MQ=255) cATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCt > 1:3769776/1‑52 (MQ=255) cATACAGTAACATCAACTCCCCCATCGTATCGCCAACCACAACCTg < 1:2212210/46‑1 (MQ=255) aTACAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCt < 1:2081645/51‑1 (MQ=255) tACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGc < 1:394085/49‑1 (MQ=255) tACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAGGGGACTTCCCCTGa < 1:2739631/71‑1 (MQ=255) tACAGCAACATCAACTCGCCCATCGTATCGCCAACcaca < 1:1344269/39‑1 (MQ=255) aCAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggtg < 1:1089470/53‑1 (MQ=255) aCAGCAACATCAACTCGCCCATCGTATCGCCAACcac < 1:1305791/37‑1 (MQ=255) cAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAGGGGACTTCCCCTg > 1:4361236/1‑68 (MQ=255) cAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAGGGGACTTCCCCTGAag < 1:216347/71‑1 (MQ=255) cAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAg > 1:489492/1‑55 (MQ=255) cAGCAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAGGGGACTTCCCCTg > 1:2492078/1‑68 (MQ=255) gTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAGGGGACTTCCCCTGAagag < 1:2129634/71‑1 (MQ=255) tAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCtggtg < 1:2824271/49‑1 (MQ=255) tAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAGGGGACTTCCCCTGAAGAGc > 1:4083113/1‑71 (MQ=255) | CAGCGGATTATGCCCACCACGTTCAACCAGTGAACCGCCAACAAAGGCGAGATCGGCAATGCCATACAGTAACATCAACTCGCCCATCGTATCGCCAACCACAACCTGCGTGCTGGTGGAGGGGACTTCCCCTGAAGAGC > minE/2531925‑2532064 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |