Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	747,442:1	+T	100%	intergenic (+49/+13)	ycfJ → / ← ycfQ	hypothetical protein/predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	747,442	1	.	T	100.0%	137.0 / NA	42	intergenic (+49/+13)	ycfJ/ycfQ	hypothetical protein/predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (42/0);  total (42/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCATTCGCTCAGGCTGAGGGGC‑TTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGT  >  minE/747421‑747490                       |                                                 tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCCCCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1611816/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGt                         >  1:929881/1‑48 (MQ=38) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCg                      >  1:641869/1‑51 (MQ=38) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGt     >  1:1541998/1‑68 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTctt  >  1:1175034/1‑69 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCg   >  1:116016/1‑70 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:2548420/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:2382387/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:2561324/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:256520/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:26616/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:2749566/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:2837554/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:2921644/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:387649/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:392893/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:523758/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:582683/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:59434/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:805982/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:850988/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:862650/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:965657/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1738175/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1105872/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1113001/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1279329/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1313896/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1405890/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1526403/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1529992/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1624231/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1627108/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:250387/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1774199/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1896857/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:2047799/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:213029/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:2131523/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:2236011/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:1092869/1‑71 (MQ=255) tCATTCGCTCAGGCTGAGGGGCTTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGt  >  1:2403040/1‑71 (MQ=255)                       |                                                 TCATTCGCTCAGGCTGAGGGGC‑TTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGT  >  minE/747421‑747490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑