| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,093,734:1 | +C | 100% | coding (2643/3057 nt) | ydiJ ← | predicted FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,093,734 | 1 | . | C | 100.0% | 17.1 / NA | 6 | G881G (GGC→GGG) | ydiJ | predicted FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (6/0); total (6/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch. | |||||||||||
AAACTCGCCACGCTCCTCG‑CCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATC > minE/1093716‑1093784 | aaaCTCGCCACGCTCCTCGCCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGa > 1:2114415/1‑68 (MQ=255)aaaCTCGCCACGCTCCTCGCCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATc > 1:1005824/1‑70 (MQ=255)aaaCTCGCCACGCTCCTCGCCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATc > 1:2195571/1‑70 (MQ=255)aaaCTCGCCACGCTCCTCGCCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATc > 1:499277/1‑70 (MQ=255)aaaCTCGCCACGCTCCTCGCCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATc > 1:795011/1‑70 (MQ=255)aaaCTCGCCACGCTCCTCGCCCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGACGGAt > 1:1356650/1‑69 (MQ=255) | AAACTCGCCACGCTCCTCG‑CCCAGCGCCAGTTTATATTCATCGCGATAACAAAGTACCAGCGCCGGATC > minE/1093716‑1093784 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |