| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,179,295:1 | +A | 100% | coding (745/984 nt) | ansA → | cytoplasmic L‑asparaginase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,179,295 | 1 | . | A | 90.9% | 62.5 / 1.4 | 22 | coding (745/984 nt) | ansA | cytoplasmic L‑asparaginase I |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (20/0); total (20/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
ACCGGTGAAAGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCAC‑AAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACA > minE/1179246‑1179365 | aCCGGTGAAAGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCAC‑AAAACAAAGCCTTCCTGCAgg < 1:2772335/71‑1 (MQ=255)aCCGGTGAAAGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCAC‑AAAACAAAGCCTTCCTGCAgg < 1:1454989/71‑1 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTa > 1:822623/1‑68 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:1828583/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:2742641/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:2784482/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:2701672/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:2538925/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:2505701/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:2470188/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:2861959/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:2160235/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:342612/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:449452/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:726973/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:1553341/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:1455970/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:762730/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:1423533/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:1133866/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:1098102/1‑69 (MQ=255) aGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCACAAAAACAAAGCCTTCCTGCAGGAATTAc > 1:1091828/1‑69 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:2478063/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:697600/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:334184/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:747397/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:2824351/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:2807804/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:931007/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:2710856/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:2708267/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:2571922/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:1032248/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:2396355/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:2342944/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:1863488/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:184866/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:1705894/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:167547/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:1472034/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:1425993/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:1320825/71‑1 (MQ=255) c‑aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGAcaca < 1:1079109/71‑1 (MQ=255) | ACCGGTGAAAGCATTGATTCTGCGCTCCTATGGCGTGGGTAATGCGCCAC‑AAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACA > minE/1179246‑1179365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |