| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,281,598:1 | +G | 100% | coding (3377/7104 nt) | yeeJ → | adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,281,598 | 1 | . | G | 85.0% | 61.6 / 4.9 | 20 | coding (3377/7104 nt) | yeeJ | adhesin |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base G (0/17); total (3/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
ACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGC‑GGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGC > minE/1281552‑1281651 | aCTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGC‑GGGCACGCATACGGTTACCGCAAc > 1:1144703/1‑71 (MQ=255)aCTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGC‑GGGCACGCATACGGTTACCGCAAc > 1:1177989/1‑71 (MQ=255)aCTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGC‑GGGCACGCATACGGTTACCGCAAc > 1:1314528/1‑71 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:929920/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:1059301/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:87625/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:855229/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:709527/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:355872/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:303716/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:2655782/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:2277570/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:2253678/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:2074147/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:2025762/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:1931072/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:1656170/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:1284426/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:1255068/71‑1 (MQ=255) cTGAAAGGTAAAAAAGCGGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc < 1:1224970/71‑1 (MQ=255) | ACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGC‑GGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGC > minE/1281552‑1281651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |