| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,361,309:1 | +C | 100% | coding (1071/1110 nt) | mrp ← | antiporter inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,361,309 | 1 | . | C | 94.4% | 57.8 / ‑2.5 | 18 | coding (1071/1110 nt) | mrp | antiporter inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (17/0); total (17/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCA‑CCCTGCCAGTAGAGCTGAGCTGCAACGCGGT > minE/1361269‑1361340 | gTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCA‑CCCTGCCAGTAGAGCTGAGCTGCAAcgcg < 1:1995113/70‑1 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:1976576/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:783423/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:699795/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:399481/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:2848648/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:2679896/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:224354/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:1983933/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:1172957/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:1870126/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:1838682/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:1775236/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:1533781/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:1505382/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:1317106/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:1308810/1‑70 (MQ=255) aGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCACCCCTGCCAGTAGAGCTGAGCTGCAACGCGGt > 1:1305006/1‑70 (MQ=255) | GTTAGACCGCGCGGAAGGAAATCTCGCCTGGAATGACTTCA‑CCCTGCCAGTAGAGCTGAGCTGCAACGCGGT > minE/1361269‑1361340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |