| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,381,801:1 | +A | 100% | coding (196/1158 nt) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,381,801 | 1 | . | A | 100.0% | 63.1 / NA | 20 | A66S (GCG→TCG) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (20/0); total (20/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGC‑AAAAAGCGTGAGGAATTTCACCTG > minE/1381756‑1381825 | tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGa > 1:2061337/1‑58 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCAc > 1:2704076/1‑68 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:2334378/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:939386/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:582101/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:509215/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:446738/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:404707/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:291508/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:2686399/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:1006912/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:2254762/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:2162399/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:1637472/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:1550286/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:1452039/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:1107818/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:1088133/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAGCGTGAGGAATTTCACCTg > 1:1009802/1‑71 (MQ=255)tGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAAACGTGAGGAATTTCACCTg > 1:351904/1‑71 (MQ=255) | TGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGC‑AAAAAGCGTGAGGAATTTCACCTG > minE/1381756‑1381825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |