Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,602,012:1 +A 100% coding (853/1305 nt) yfeW → predicted periplasmic esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,602,0121.A84.6% 29.6 / 1.9 13coding (853/1305 nt)yfeWpredicted periplasmic esterase
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base A (11/0);  total (13/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

ATCCATTTCCCGAATATCCGCACCTCCACTCTCTGGGGTCAGGTGCACGATG‑AAAAAGCCTTTTATTCGATGGGCGGCGTTTCCGGG  >  minE/1601961‑1602047
                                                    |                                   
aTCCATTTCCCGAATATCCGCACCTCCACTCTCTGGGGTCAGGTGCACGATG‑AAAAAGCCTTTTATTCGa                   >  1:640958/1‑70 (MQ=255)
 tCCATTTCCCGAATATCCGCACCTCCACTCTCTGGGGTCAGGTGCACGATG‑AAAAAGCCTTTTATTCGATg                 >  1:1746134/1‑71 (MQ=255)
                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:1546306/1‑70 (MQ=255)
                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:1644358/1‑70 (MQ=255)
                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:1674577/1‑70 (MQ=255)
                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:2656268/1‑70 (MQ=255)
                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:2780840/1‑70 (MQ=255)
                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:317654/1‑70 (MQ=255)
                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:479529/1‑70 (MQ=255)
                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:49296/1‑70 (MQ=255)
                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:642915/1‑70 (MQ=255)
                  cGCACCTCCACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:871978/1‑70 (MQ=255)
                  cGCACCTACACTCTCTGGGGTCAGGTGCACGATGAAAAAAGCCTTTTATTCGATGGGCGGCGTTTCCggg  >  1:2456977/1‑70 (MQ=255)
                                                    |                                   
ATCCATTTCCCGAATATCCGCACCTCCACTCTCTGGGGTCAGGTGCACGATG‑AAAAAGCCTTTTATTCGATGGGCGGCGTTTCCGGG  >  minE/1601961‑1602047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑