Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,808,175:1	+A	100%	intergenic (+994/‑113)	tyrB → / → aphA	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent/acid phosphatase/phosphotransferase, class B, non‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,808,175	1	.	A	100.0%	21.4 / NA	8	intergenic (+994/‑113)	tyrB/aphA	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent/acid phosphatase/phosphotransferase, class B, non‑specific
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (8/0);  total (8/0)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

CATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCAC‑AAAAACCGCAGATAATCCTTCCTTTCCCCGG  >  minE/2808107‑2808206                                                                      |                                cATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATcac‑a                                >  1:1320693/1‑70 (MQ=255) cATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATcac‑a                                >  1:1945589/1‑70 (MQ=255) cATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATcac‑a                                >  1:2107302/1‑70 (MQ=255) cATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATcac‑a                                >  1:2728266/1‑70 (MQ=255) cATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATcac‑a                                >  1:778616/1‑70 (MQ=255) cATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATcac‑a                                >  1:874400/1‑70 (MQ=255) cATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATcac                                  >  1:196503/1‑69 (MQ=255)                                tttACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAAACCGCAGATAATCCTTCCTTTCCCCgg  >  1:1442497/1‑70 (MQ=255)                                tttACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAAACCGCAGATAATCCTTCCTTTCCCCgg  >  1:1595956/1‑70 (MQ=255)                                tttACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAAACCGCAGATAATCCTTCCTTTCCCCgg  >  1:2280162/1‑70 (MQ=255)                                tttACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAAACCGCAGATAATCCTTCCTTTCCCCgg  >  1:2360809/1‑70 (MQ=255)                                tttACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAAACCGCAGATAATCCTTCCTTTCCCCgg  >  1:2411229/1‑70 (MQ=255)                                tttACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAAACCGCAGATAATCCTTCCTTTCCCCgg  >  1:2444801/1‑70 (MQ=255)                                tttACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAAACCGCAGATAATCCTTCCTTTCCCCgg  >  1:2898986/1‑70 (MQ=255)                                tttACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAAACCGCAGATAATCCTTCCTTTCCCCgg  >  1:683887/1‑70 (MQ=255)                                                                      |                                CATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCAC‑AAAAACCGCAGATAATCCTTCCTTTCCCCGG  >  minE/2808107‑2808206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑