Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	minE	2164055	2164064	10	30 [0]	[0] 4	yrbE	predicted toluene transporter subunit

TTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGC  >  minE/2163984‑2164054                                                                       | tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACTACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:1654892/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:105364/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:969536/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:811608/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:661228/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:636325/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:621917/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:611885/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:460874/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:45542/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:413150/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:2766872/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:2553541/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:2319814/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:1009518/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:1091155/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:1597821/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:1909017/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:2104986/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:2223761/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:2251872/71‑1 (MQ=255) tttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAAAGCGGCAACCACCGGCCCCAGTTCACGc  <  1:72191/71‑1 (MQ=255) tttCTGCGGTTAGCACCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:463171/71‑1 (MQ=255) tttCTGAGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:2820126/71‑1 (MQ=255)  ttCTGCGGTTAGCGCCGAACCAGCACGCCCGTCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:2335898/70‑1 (MQ=255)  ttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:420048/70‑1 (MQ=255)  ttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:1728976/70‑1 (MQ=255)  ttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:1231312/70‑1 (MQ=255)  ttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:780173/70‑1 (MQ=255)                   aaCCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGc  <  1:307208/53‑1 (MQ=255)                                                                       | TTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGC  >  minE/2163984‑2164054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑