| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 93,020 | T→A | 100% | M1K (ATG→AAG) † | secA → | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 93,020 | 0 | T | A | 100.0% | 54.4 / NA | 18 | M1K (ATG→AAG) † | secA | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (18/0); total (18/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCGCAACGCGGGGCGTTTGAGATTTTATTATGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAC > minE/92990‑93060 | ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTa > 1:1065592/1‑69 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:942301/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:1019296/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:569900/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:453214/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:332584/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:317917/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:312879/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:2812976/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:2809723/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:2792395/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:2438963/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:1990868/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:1675785/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:162131/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:1485880/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:1435151/1‑71 (MQ=255)ccGCAACGCGGGGCGTTTGAGATTTTATTAAGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAc > 1:1114340/1‑71 (MQ=255) | CCGCAACGCGGGGCGTTTGAGATTTTATTATGCTAATCAAATTGTTAACTAAAGTTTTCGGTAGTCGTAAC > minE/92990‑93060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |