Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 353,513 T→A 100% intergenic (‑1/‑173) ppiB ← / → cysS peptidyl‑prolyl cis‑trans isomerase B/cysteinyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE353,5130TA86.5% 201.6 / 22.5 74intergenic (‑1/‑173)ppiB/cysSpeptidyl‑prolyl cis‑trans isomerase B/cysteinyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (10/0);  new base A (64/0);  total (74/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCATATATAGGGTGGTGTTA  >  minE/353486‑353577
                           |                                                                
gCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCgg                       >  1:980183/1‑71 (MQ=255)
gCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCgg                       >  1:2305932/1‑71 (MQ=255)
gCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCgg                       >  1:2698343/1‑71 (MQ=255)
gCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCgg                       >  1:739089/1‑71 (MQ=255)
gCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATACTGTTCCGTTTGATTGGTGCTTCAACCCAGTTAgg                       >  1:2916527/1‑71 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTTTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTc                    >  1:774556/1‑69 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTc                                                    >  1:581812/1‑37 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGtt                          >  1:2735501/1‑63 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGt                           >  1:2675823/1‑62 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGt                     >  1:474873/1‑68 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2458937/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:956397/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2671128/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2626050/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:255805/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2549951/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2475374/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2733434/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2434102/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2398035/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2382762/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2325491/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:105851/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2725517/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2731456/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:852482/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2829655/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2842022/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2888839/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2891055/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:420123/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:464167/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:64917/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:716647/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:85452/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:79301/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1368890/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1758754/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2247899/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1739432/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1725805/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:160761/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1591525/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1582894/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1504850/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1751510/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1278142/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1258142/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1217991/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1165076/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1099113/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1064469/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1059835/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:105776/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1873552/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2235141/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2185171/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2165652/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2138889/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:212049/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:211785/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2043988/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:2025393/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:201176/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1993173/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1964464/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1889500/1‑70 (MQ=255)
     gATTGGTGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:1877288/1‑70 (MQ=255)
     gATTGGAGTGGAAAGTAACCATATTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCa                   >  1:5053/1‑70 (MQ=255)
         ggTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCatata               >  1:2213277/1‑70 (MQ=255)
         ggTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCatata               >  1:881680/1‑70 (MQ=255)
         ggTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCatata               >  1:234168/1‑70 (MQ=255)
                     aaCCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCATATATAGGGTGGTGTta  >  1:458995/1‑71 (MQ=255)
                     aaCCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCATATATAGGGTGGTGTta  >  1:1833130/1‑71 (MQ=255)
                           |                                                                
GCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCATATATAGGGTGGTGTTA  >  minE/353486‑353577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑